We narrowed to 1,010 results for: RING;

1. Fluorescent Tagging of Endogenous Genes with SapTrap

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   ...and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles...Cas Plasmids - Protein Tagging. Beyond simply monitoring protein localization and activity, CRISPR tagging...

2. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   ...FPs that dimerize can form dynamic contacts that bring apposing membranes together and can wrap the membranes...reporter that undergoes a conformational change to bring two FPs on a single protein closer together or further...

3. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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   ...similar resource for those involved in monocot engineering projects. The MoClo tools will work in any dicot...think plant biology is massively underfunded considering how important it is. I would really like to reach...

4. Simplify Cloning with in vivo Assembly

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   ...to get into the same cell. For simple cloning requiring just one or two recombination events, homemade...DNA polymerase This will minimize random errors during PCR: we recommend Phusion or Q5. Speed up your ...

5. Great Results Start with Great Standard Curves

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   ...step of the series is consistent at 1:2. When preparing a dilution series, use a new pipette tip for each...components can affect the final readout. When preparing a standard curve, change pipette tips and mix ...

6. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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   ...community is a kind of duty for researchers when considering the peer-review system of journals and grant ...technologies developed abroad, so it's a little boring. I think that such tendency is one of the reasons...

7. Negotiating Work and Life: How to Find the Joy

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   ...Neighborhood.   Work/life balance. Is it truly possible to bring all aspects of our lives under control? Balance ... friends. If there is any guilt, you should be sharing it absolutely equally, which means you only have...

8. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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   ... children, it can also be applied to research. Sharing knowledge and tools is the best way to help propel...tools, and resources. Luckily for us, they like sharing! You can find data, analysis tools, and other scientific...

9. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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   ...full-length (19-20 bp) or short (17-18 bp) guides. When comparing the toxicity of guides that have 1-bp mismatch...Central PMCID: PMC3988262. 4. Hart, Traver, et al. "Measuring error rates in genomic perturbation screens: gold...

10. Plasmids 101: Shuttle Vectors

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    ...biology because of an image that was honestly quite boring. My Bio 101 professor displayed a codon table —...mammalian cells like neurons, may not replicate during the course of your experiment, meaning you don’...