We narrowed to 1,010 results for: RING;
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TypeBlog Post... target guanosine instead, perhaps analogous engineering of UNG, which excises uracil, could allow it ...considerably. And there’s so much more exciting protein engineering going on in the base editing field! From CRISPR-free...
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Plasmids 101: Broad Host Range Plasmids
TypeBlog Post...inducible promoters with different degrees of stringency and inducibility (Toh et al. 2023), and additional...across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit... -
How to Write a Scientific Review Article
TypeBlog Post...about. Instead of reading a paper and getting my bearings, I would read a paper, panic, and then download...think the field is going or what areas are worth exploring. Edit and rewrite, then repeat If you’re like... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog Post...mesothelioma. Asbestos, and certain other naturally occurring asbestiform minerals such as erionite, are the...an immediate stop to the use of asbestos, thus ensuring that future generations will be protected from... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog Post...pinhead—contribute to the “sculpting” of tissues and organs during embryo development. Single cell sequencing-based...Single-cell reconstruction of developmental trajectories during zebrafish embryogenesis." Science 360.6392 (2018... -
Hot Plasmids - August 2020
TypeBlog Post...repressing and activating expression of TCF4 while monitoring transcript and protein levels. Plasmids for multiplexed...donor infection (CRISPR-READI) enables genome engineering using HDR donors lengths up to 4.9 kb. Find ... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...blog post). Optimizing FLP FRT through smart engineering Different FLP versions – making FLP efficient...Frank et al. 2003. “A Directional Strategy for Monitoring Cre-Mediated Recombination at the Cellular Level... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...Vader in "Return of the Jedi" While Vader was referring to the evil side of a mystical "Force," this quote...posttranslational modifications can disrupt FP folding rendering the protein non-fluorescent. Unfortunately, accumulated... -
Plasmids 101: Control Plasmids
TypeBlog Post...the plasmid without the independent variable. Referring back to the experiment associated with Figure ...aliquot/preparation. The replicate control isn’t measuring or assessing reproducibility of the effect of ... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog Post...high protein levels including: ompT: Strains harboring this mutation are deficient in outer membrane ...recommended as these auxillary plasmids may be isolated during the prepping process. How does inducible expression...