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We narrowed to 138 results for: THY-2

Showing: 71 - 80 of 138 results

  1. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... is also gaining a lot of new traction lately (1, 2), depends on knowledge of the structure(s) a given...per plate) across 20 fresh selective plates. Step 2: Express random phage DNA fragments in E. coli Now...microbiology 23.4 (2015): 185-191. PubMed PMID: 25708933. 2. Cooper, Callum J., Mohammadali Khan Mirzaei, and ... biology 34 (2015): 69-77. PubMed PMID: 26275959. 2. Weigele, Peter, and Elisabeth A. Raleigh. "Biosynthesis...because phages frequently alter their DNA by adding methyl groups, glycosyl groups, and other modifications...

  2. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...one injection step! The entire protocol takes about 2-3 weeks but requires less than eight hours worth of...for C. elegans genome modification. Figure 2: Schematic from Dickinson et al. (2015) illustrating...experiments, but this strategy required the use of unhealthy, difficult-to-inject unc-119(ed3) animals, as ...

  3. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...Genome Editing and The CRISPR Software Matchmaker Day 2: Homology directed repair: CRISPR 101: Homology Directed...histone demethylation by LSD1, cytosine methylation by DNMT3A or MQ1, and cytosine demethylation by Tet1...

  4. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...improve solubility and stability (Figure 2).      Figure 2: Crystal structures of CRBNmidi alone...fluorescent tags in Caenorhabditis elegans. Genetics, 228(2), iyae126. https://doi.org/10.1093/genetics/iyae126...for CRISPR/Cas9 in M. abscessus here! Neo D, Clatworthy A, and Hung, D. (2024). A dual-plasmid CRISPR...

  5. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...Interview with Stem Cell Researcher Darrell Kotton 2. Stem cell scientists care about patients As any grad... Yamanaka explained that it took 50 people nearly 2 years to create the first stem cell line that could...they are competing with each other, but it's a healthy competition. What an honor for Addgene to be a ...

  6. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...represent a favorable state for tumor progression (Ref 2). Future treatments may need to target this immunosuppressive...cell 30.1 (2016): 147-160. PubMed PMID: 27411589. 2. Todoric, Jelena, Laura Antonucci, and Michael Karin...Professor Kroemer often functions as one of the “healthy human volunteers” in his studies! This was followed...

  7. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ... a western, proteins are: (1) separated by size, (2) transferred to a membrane, and  (3) detected using...expensive system-specific sandwiches.   Figure 2: The western blot dry apparatus includes a stack of...modifications such as phosphorylation, acetylation, methylation and ubiquitination. For example, to test if a...(1979) Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method...

  8. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...without leaving behind any accessory DNA sequences [2], [3]. Simply put, the piggyBac™ transposon containing...with a Footprint-Free™ genome edit.   Figure 2: Editing DNA using the piggyBac™ transposon selection...PMID: 26211958. PubMed Central PMCID: PMC4663986. 2.  A. M. Singh, V. V Adjan Steffey, T. Yeshi, and D...containing a negative selection marker such as thymidine kinase, a researcher may subsequently kill off...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter. Addgene...backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed. The authors then used GMAP assembly...engineering (MAGE) (1). MAGE uses recombineering (2) to simultaneously incorporate multiple single-strand...number of tandemly expressed gRNAs respectively) and 2) the size of the deletion. Smaller deletions generally... that locus to be methylated. This powerful tool has been used to direct methylation to and lower expression...recombinase enzymes need to be expressed and the native methyl-directed mismatch repair (MMR) system needs to ...Repurposing the CRISPR-Cas9 system for targeted DNA methylation The traditional approach to studying epigenetic...

  10. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...efficient knockout (KO) of genes in immune cells; (2) is not immunogenic, a necessity for in vivo studies... and pooled screening approaches; (4) encodes a Thy1.1 selectable marker to facilitate the isolation of...
Showing: 71 - 80 of 138 results