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...sterile conditions where disturbing the tissue for imaging or collection could contaminate it. I have used...self-cleaving 2A site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al. Hortic Res... highly expressing RUBY plant is a vibrant red/magenta while (bottom panel, left) a lower expressing RUBY... He has a passion for microscopy and beautiful imaging. References and Resources References  He, Y., ...

...penetrance and live cell imaging capabilities. Upon developing a new imaging-based bacterial colony screening...fluorescent proteins were the state of the art in RNA imaging in vivo. These new “Riboglow” probes contain a ...and achieve better signal in vivo as compared to imaging with broccoli aptamers or MS2 tags using the same...

...will produce a secondary color (yellow, cyan, or magenta) depending on the hairpin combination. A gRNA containing...the same sgRNA will generate additional colors. Image from Ma et al., 2016 with permission.   Developing...-arm (T1), and the q-arm (T2) of chromosome 19. Image from Ma et al., 2018 with permission.   Why...RNA scaffolds for signal amplification in genome imaging.” Nature Methods 15 (2018): 928-931. PubMed PMID...

... event. What are exosome AAVs? In 2012, Casey Maguire and colleagues from Massachusetts General Hospital...that are released from cells during production (Maguire et al., 2012). They saw that these AAV particles...development of the exo-AAV isolation protocol by Maguire and colleagues, other researchers have tried to...: 28082074. PubMed Central PMCID: PMC5368844.  Maguire, Casey A., et al. "Microvesicle-associated AAV ...

...membrane. The haemagglutinin (HA) tag is a small 9 amino acid epitope of the haemagglutinin of the influenza...membrane.   Figure 1: Electron microscope images showing ultrastructural localization of hM4Di in...coupled to mCherry (top) or HA tag (bottom). The images show examples of dendrites (blue shades) in the...

...and co-transfected into cells for reconstitution. Image from Levy et al., 2020 with permission. Though...quenches a proportion of mScarlet's emission via FRET. Image from Beck and Gong, 2019. Broadening the spectral...and subsequent transformation into cyanobacteria. Image from Vasudevan et al., 2018. Find the CyanoGate...binding, bringing the two halves of Cre together. Image from Eisenhoffer et al., 2019. This novel tool...

...   Figure 2: Inner mitochondrial membranes imaged with structured illumination microscopy in HeLa...COX8a-mStayGold. Scale bars 10 μm (main), 1 μm (inset). Image reused from Ando et al. 2023, under CC-BY license...identification and characterization of AAV.CAP-Mac. Image reproduced from Chuapoco, Flytzanis, Goeden, et ...the most versatile. Whether you plan to purify or image a recombinant protein, the FLAG tag has proven itself...

...for certain applications such as in vivo tissue imaging.   Figure 1: Ace-mScarlet is a fusion ... a proportion of mScarlet's emission via FRET.  Image from Beck et al, 2019 under CC BY 4.0.   FRET...This system is particularly powerful for in vivo imaging especially since JFs can cross the blood brain ...targeting your cell type of interest. For in vivo imaging applications, the ability to direct your voltage...

... antigens as measured by suspension bead assay. Image from  Preger et al., 2020. Preger et al., Journal...3: Schematic representation of the MPyV system. Image from Cheah et al., 2021.   After validating ... vectors expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2... terminal providing better signal to noise for imaging calcium dynamics in fine structures. We have expanded...

... the nuances of protein structure in a flat 2-D images. In the early days of structural biology, Byron...allowed researchers to create stunning, interactive images of their proteins. Since the 1980’s, journal articles...articles have been filled with impressive, full-color images of proteins. But they are still flat. To truly ...educator, these instances of focused attention become magical teaching moments. The models function as thinking...

...engineering of biology much easier. So you could imagine engineering the entirety of a cell. That's a big... or so sensor domains for everything you could imagine. That I think is the next priority; we need the...clear, but the way I'd leave it is mostly to the imagination. The question is: what can you use biology to...off-the-shelf genetic elements that allow you to begin to imagine designing 10-gene systems with quantitative precision...

... Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com Science communication should...enough information to tell a compelling story in an image or do you need a full exhibit? In a five minute ... to convey an entire story or context via still images. Some things cannot be photographed or need text...interpretation. Infographics Low Combines both words and images; especially useful for conveying and contextualizing...

...mCyRFP1: Novel fluorescent protein that enables imaging of two biosensors simultaneously   Researchers...energy transfer) and FLIM (Fluorescence Lifetime Imaging Microscopy) applications. To demonstrate the power...shown great performance in cellular functional imaging and in protein fusions. The Gadella lab has deposited...toolbox of fluorescent proteins (FP) for cellular imaging is constantly expanding. Case in point - check ...could be better suited for deep-tissue in vivo imaging. These variants were developed as part of a larger...

...Cas9-mediated editing (ACE) reporter in action. Image from St. Martin et al., 2018. APOBEC-Cas9n-UGI...Editing efficiencies using base editing reporters. Image from St. Martin et al., 2019. The Harris lab ...results in shift in emission spectra from BFP to GFP. Image from Standage-Beier et al., 2019. The team put...luciferase reporter, and neomycin resistance reporter. Image adapted from Katti et al., 2020. As a proof of...

...temperature than its Spinach ancestors, and has a lower magnesium dependence. Though initially still unstable in...Central PMCID: PMC4364232. 2. Baker, Monya. “RNA imaging in situ”. Nature Methods 9, 787–790 (2012). 3. .... Filonov, Gregory S., et al. “In-gel imaging of RNA processing using broccoli reveals optimal aptamer...PMCID: PMC4071757. 6. Hong, He, et al. “Molecular Imaging with Nucleic Acid Aptamers.” Curr Med Chem.  2011...

...non-fluorescent, and misfolded FP-fusions on quantitative imaging experiments and cell physiology in general. Pitfalls...readily apparent and can confound quantitative imaging experiments or even negatively impact cells (see... represent one of the most inert solutions for imaging in a variety of cellular compartments including...proteins in the Endoplasmic Reticulum and live cell imaging approaches. Erik is also an avid long distance ...

...material is a factor of practicality (given image sizes) but images should be unprocessed and in capture format...other than the actual author. Instead, selected images are said to be representative or the statistical...charts and tables in the paper are in scope. For images, the number of examples supporting the published...

...blocking, and washing, the membrane is reassembled and imaged. In this example, a 1:1,000 dilution gives a high...eye; a fluorescent ladder compatible with your imaging system; or a combination of the two. There are ... wash for the full amount of time, every time. Imaging The last step in a western blot tends to be the...technical design of a western blot”) post, most imaging issues can be fixed real-time by exposing for either...seeing high background or low signal during the imaging process, try adjusting your exposure time first...

...Scientists around the world began looking for the magic molecule that serves as the code of life. In 1944...Raymond Gosling took the first ever X-ray diffracted image of the DNA. Finally, in 1953, Franklin along with...the two most popular topics that sparked the imagination of most of the authors during this period.  Genetic...engineering in pop culture SciFi is one of the most imaginative and most important genres of literary fiction...

...of regenerative medicine is to replace lost or damaged cells. Here, I will discuss the two major processes... is observed in the pancreas when excess β-cell damage results in the transdifferentiation of glucagon-producing...cells from the patient in vitro then correct the damaged gene in the resulting iPSCs before differentiating...to replenish and repair cells that are lost or damaged. Both transdifferentiation and reprogramming give...