We narrowed to 345 results for: Ada

1. Truncated gRNAs for Regulating Gene Expression

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   ...interacts with a ≤15nt gRNA that contains an RNA adapter capable of recruiting a transcriptional activator...

2. Plasmids 101: Knockout/Knock-In Plasmids

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   ...them by depositing with Addgene! Figures were adapted from: Hall B, A Limaye, & AB Kulkarni. (2009) "...

3. Experimenting with New Careers while in Grad School

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   ...online science communicators like Phil Plait @BadAstronomer, Emily Graslie from The Brain Scoop YouTube ...

4. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...medicine. The parts we have right now are well adapted to work in prokaryotic bacteria, not in fungal ...

5. SciComm: Taking Science to Elementary and Middle Schools

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   ...formulated the presentation as follows (which could be adapted for any topic--not just CRISPR): Who am I? Where...

6. Capturing a Moment with Marc Zimmer’s “The State of Science”

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   ...difficulties in dealing with climate change, the degradation of the U.S. environment, and a decline in the...

7. Getting to Know Expansion Microscopy

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   ...model systems, paired with SRM techniques, and adapted for detecting nucleic acids as well as proteins...

8. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   ...given state or point in time. You want to halt degradative processes while maintaining the structure of ...

9. A Guide to Starting Your Own Journal Club

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   ...group and topic. For example, it may be useful to adapt the second version (multiple presenter, figure by...

10. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ...are quickly cleared from the cell via protein degradation pathways. This makes Cas9 RNPs useful for CRISPR...

11. AAVs in Retinal Gene Therapy

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    ...efficiently transduce various types of retinal neurons. Nowadays a toolbox of naturally occurring and engineered...

12. The Importance of a Fun Workplace: Company Culture at Addgene

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    ...fashion. 9. Ask for help My grandma used the old adage “many hands make light work” all the time. She was...

13. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...immunoprecipitation (enChIP). Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H. Sci Rep. 2013 Nov ...

14. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    ...Genetic compensation triggered by mutant mRNA degradation." Nature 568.7751 (2019): 193. PubMed PMID: 30944477...

15. Fluorescent Proteins 101: Aptamer Fluorophores

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    ...nucleotide-based ligand binders would prove to be highly adaptable tools. The key to aptamers’ flexibility is their...

16. Antibodies 101: The Four ELISAs and When to Use Them

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    ...measure antibody levels, scientists have since adapted it for a host of different proteins and small molecules...

17. Plasmids 101: Stringent Regulation of Replication

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    ...Figure 2: Prokaryotic chromosomal replication. Adapted from BioRender template. Since DnaA binding...

18. Plasmids 101: Broad Host Range Plasmids

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    ...contains structural elements that are versatile and adaptable. Variation in ori structure allows plasmids to...

19. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    ...pages. We guarantee this will save you a lot of headaches down the line! Cash in on gene synthesis Before...

20. An Inside Look into Addgene's Dev Team

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    ...in iterative chunks, we have the flexibility to adapt and easily respond to changing needs of the teams...

21. Plasmids 101: Codon usage bias

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    ...structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms often need...

22. Neuronal labeling with Spaghetti Monster

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    ...smFPs in the future. References 1. Viswanathan, Sarada, et al. "High-performance probes for light and ...

23. Mesothelioma - Causes, Symptoms, and Treatment

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    ...like the Asbestos Disease Awareness Organization (ADAO) are working toward such a ban, and the president...

24. Antibiotic Resistance: An Old Solution but a New Problem

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    ...bacteriology and drug resistance.  It seems as though, nowadays, we cannot make it a full month without a new,...

25. Using Video to Share Your Science: We Share Science

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    ...published in scientific journals flies under the radar and gains little notice. A 2009 research study found...

26. CRISPR Activation: A Practical Guide

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    ...doi.org/10.1038/nmeth.3871 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes...

27. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    ...that a fusion protein is cleaved by proteases. Nowadays, with ample structural information available, ...

28. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    ... visualization of two colors. Similarly, Flybow adapts Brainbow 2.0 for use in Drosophila melanogaster...

29. Five Popular Model Organisms, Part 2

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    ...plant species and metabolic properties affecting adaptations.  Arabidopsis thaliana flowers. Image from...

30. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    ...solution is to include an in-frame degron, or degradation sequence, upstream of the 5’ splice donor and...

31. Quick Guide to Near-Infrared Fluorescent Proteins

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    ... changes occurring due to protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova ...

32. Plasmids 101: Expressing shRNAs for RNA Interference

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    ...(2021). Life of RISC: Formation, action, and degradation of RNA-induced silencing complex. Molecular Cell...

33. Antibodies 101: Normalization and Loading Controls for Western Blots

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    ...the gel (n=3). Data is presented as mean ± SEM. Adapted from Analytical Biochemistry, vol 440, Gilda, J...

34. Plasmids 101: Biotinylation

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    .... The biotin-avidin interaction has also been adapted for use in immunohistochemistry and antibody labelling...

35. Adeno-associated Viruses (AAVs) for Genome Editing

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    ...and pAAV-TK-Acceptor) so that they can be easily adapted to edit your gene of choice. These vectors are ...

36. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    ...parts. Gibson assembly meets CRISPR Gibson can be adapted to more complicated cloning schemes, such as those...

37. Treating Muscular Dystrophy with CRISPR Gene Editing

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    ...low-frequency indels will not cause frameshifts. Figure adapted from Osterout et al. (2015b) under a CC-BY 4.0 ...

38. Viral Vectors 101: Optogenetic Tools

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    ...10.1016/j.sbi.2019.05.006 Vierock, J., Rodriguez-Rozada, S., Dieter, A., Pieper, F., Sims, R., Tenedini...

39. Viral Vectors 101: AAV Variables That Matter

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    ...Brans, M. A. D., Luijendijk, M. C., Garner, K. M., & Adan, R. A. H. (2010). Optimization of Adeno-Associated...

40. Antibodies 101: Multiplex Immunofluorescence

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    ...C3 show magnified detail of the box in C. Image adapted from Andrews et al., 2019 under CC-BY 4.0 license...