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We narrowed to 801 results for: MAK

Showing: 281 - 320 of 801 results

  1. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...value. Types of biosensors So now that we know what makes up a biosensor, we can discuss what flavors of sensors...difficult to achieve in whole organisms, but AAVs make it possible. In AAV systems, different serotypes...expression and downstream monitoring. Nervous about making a virus for your biosensor? Don’t be! We have you...viral resources and safety tips. Still worried? We make virus with some of our most popular biosensors already...

  2. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...character you painted in your personal statement. Make certain to choose your recommenders based on your...individuals are inundated with requests. The easier you make it for them, the likelier you will end up with a...lighting for a smooth performance. Most importantly, make certain that everyone you live with is aware of ...consistent with your personal statement, while you make the ultimate case: why you? 7. Strength in numbers...

  3. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ... task will keep you from repeating your work or making similar mistakes in the future. Stay organized...camelCaseFormat since spaces between each word can make it difficult to accurately call a file. Version...your exact changes. Alternatively, you can simply make a note in your methods section about the edit that... Your Experiments Try out the CRISPR Software Matchmaker Additional Resources on Addgene.org Find Protocols...

  4. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...behind. While commercial kits are available for making small point mutations, large deletions/insertions...Current SDM methods rely on in vitro assembly. To make a point mutation, you design two oligonucleotide...instead of relying on in vitro assembly, they could make the E. coli do the work by using the principles ...-12 kb range. REPLACR-mutagenesis also requires making your own electrocompetent cells in which the phage...

  5. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...between blue and white colonies. Take care in making your plates: X-gal is light and temperature sensitive...autoclaving. If spread on top of pre-made plates, make sure it is evenly distributed and allow sufficient...specific analysis, like PCR or restriction digest. Make sure you use a proper E. coli strain (i.e. contains...JM109, STBL4, JM110, and Top10 are a few examples. Make sure you use a proper plasmid (i.e. contains the...

  6. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...how the new kit works, the research the kit now makes possible, and how it compares to his CRISPR/Cas9...step. The kit also has other unique aspects that make it especially well-suited for use in stem cells....hands of pretty much all investigators. Our kit makes it extremely simple to design TALENs. It only takes...apples-to-apples comparison of all those mutations. If you make induced pluripotent stem (iPS) cells from patients...

  7. RNA Extraction Without A Kit

    Type
    Blog Post
    ...scientists commonly rely on RNA isolation kits to make their life easier. Recently, we published a blog...cold and work quickly to mitigate RNA degradation. Make sure to use DEPC-treated or RNAse free water. If..., 2006).  View our protocol for RNA extraction! Making the simple protocol even more foolproof (still ... if you need really clean RNA, it’s important to make sure that when extracting, to only take the aqueous...

  8. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ... see who you are when you look at their profile. Make your professional contact information available.... be important for a good friend, but if you are making a professional inquiry, you always want to read...type of connection can help you gather information, make introductions, and give advice for your interviews...should be as good or better than your resume Don’t make the mistake of having a shoddy profile and not updating...

  9. Antibodies 101: Conjugation

    Type
    Blog Post
    ...properties of the fluorophores you are considering to make sure that they’ll be compatible with your instruments...properties allow for efficient signal amplification, making biotin conjugated antibodies good options for detecting...antibody. 2) Endogenous cysteines, such as those that make up the disulfide bonds between antibody chains. ...antibodies yourself, there are kits available to make the process quick and easy. Before getting started...

  10. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...experiments, and here we will focus on a unique gene that makes the popular GatewayTM method possible: ccdB. But... more about it? Read on to find out how ccdB can make your cloning experiments a little easier. One of...contain your insert of interest. Simply stated, ccdB makes cloning easier by selecting against vectors that...not contain the recombinant plasmid and therefore makes the cloning process much more efficient, as one ...

  11. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...address the research challenge. Now is the time to make bold statements. While some of the statements may...6-12 months to give feedback on the application. Make sure to invest time and consider the reviewer's ...the next application. Although some comments may make it clear that the reviewer misunderstood the point...that was being made, take it as an opportunity to make clearer statements on the next round of applications...

  12. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...or a G to A change on the opposite strand). ABEs make an A to G change (or a T to C change on the opposite...catalytically dead or nickase) fused to a deaminase that makes the edit A gRNA targeting Cas to a specific locus...efficiency for mammalian edits is to ensure the editors make it into the nucleus and that they are expressed ... 98-99% target modification in primary T cells, making them a promising tool for cell therapy applications.... J., Murray, R., Packer, M. S., Rinaldi, C., Slaymaker, I. M., Yen, J., Young, L. E., & Ciaramella, G...

  13. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ...changes (Figure 1), but it can be challenging to make these base changes, especially in primary human ....20-m to edit primary human T cells efficiently makes it a promising tool for T cell therapy development...robust editing in human cell lines and primary cells makes them a great addition to the base editing toolbox..., Liquori AJ, Murray R, Packer MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G (2020) Directed...

  14. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    ...for antibodies. Recombinant antibody technology makes it easy for scientists to share, remix, store, and...     How are Recombinant Antibodies Made? To make recombinant antibodies, you first need to know the...  Second, plasmids are easy to store and share, making recombinant antibodies the most practical choice... encoding antibodies, scientists will be able to make higher affinity antibodies, modify antibody function...

  15. Tips for Technical Support Calls

    Type
    Blog Post
    ...Tweeting and texting, actually writing an e-mail or making a phone call is a big commitment when you’re busy... to consider before you commit: Double check to make sure the answer to your question didn’t come with...expedite and improve your customer service experience: Make sure you’re contacting the correct company. This...as possible about the issue. For Addgene support, make sure to mention the plasmid catalog number, or at...

  16. Transferable Skills: Negotiation

    Type
    Blog Post
    ... jobs needed to be done (Who is responsible for making the running buffer this week?). Scientists negotiate...were hoping that your lab mate would have time to make comments on your manuscript. If you give her this...practice and hone your skills. This practice will make negotiating feel more natural. Creative negotiation...the person/team for help? What can I offer them to make it a win-win situation instead of just extra work...

  17. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...limiting reagent for my experiments, so I always make more AAV than I think I need. Unlike lentivirus,.... If you’re new to creating gradients, practice making the iodixanol layers before doing your first purification...purified and buffer exchanged virus, remember to make one or two small aliquots to use for qPCR titering...Additional resources on the Addgene blog Find out what makes AAV a versatile tool for gene expression in mammals...

  18. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...Jacob Corn contacted us in 2014 to ask if we could make a model of Cas9 based on the structure published...Jennifer Doudna’s new book A Crack in Creation. Making models more useful through flexibility It is common...the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce off-target hits in a ...our hands-on teaching tools that are designed to make the molecular world real for students. Many thanks...

  19. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...to help make the most of these pages, we've written this post to guide you through them and make the best...can be found in this location as well. These maps make it easy for scientists to see if the plasmid would...their plasmids are used in a publication. Addgene makes this easy by providing a template citation for you...

  20. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...more CRISPR tools are being published each month, making CRISPR a great choice for your next experiment!...control gene expression. Base editors can help you make precise point mutations near the gRNA target site...single-stranded DNA oligonucleotide, so you will need to make sure your expression system and delivery methods...Envelope plasmids provide the necessary components to make lentiviral particles. Stable, tunable expression...

  21. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...Science: What the Future Holds and the Scientists Making It Happen, is an exercise in restraint. The very...topics without some editorializing, and he wisely makes no such attempt. He is transparent about his own...be shared by fellow scientists--but that doesn’t make his introduction to these topics any less effective... revelations. The issues he discusses have been making the rounds in journal editorials, science social...

  22. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...applications, and the quality of a standard curve can make or break an experiment. Here, we will provide an...the concentration of the unknown sample.  How do I make the standards for a standard curve? You create a...with BioRender.com.   When choosing a standard, make sure that the sample is pure and free of any contaminants...start with a higher concentration of standard or make smaller dilutions.        As mentioned above, it...

  23. Tips for arabidopsis transformation

    Type
    Blog Post
    ...messy, so you want to make sure you have sufficient volume to dip all your plants. Make sure your Agrobacterium...agrobacterium: Getting a transformant takes a long time. Make sure the agrobacterial culture you are growing definitely...increase background growth of non-transformants and make finding true transformants more difficult. Don’...

  24. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...chemists and biochemists have developed techniques to make labeling chemistry easier and more functional in...and continual improvement of fluorescent proteins makes them the go-to choice for many (if not most) fluorescence...labeling strategies and improved fluorophores are making chemical dyes increasingly attractive and accessible...Medical Institute. He would rather be in the lab making new fluorophores for advanced imaging modalities...

  25. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...lab-use tablet? The software also makes it easy to annotate protocols, make them public for others to use,...of useful molecular cloning protocols, including making LB agar plates, restriction digest of plasmid DNA...bench, keep your inventory at your finger tips, and make shopping lists as you finish the last of a reagent...

  26. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...aggregation and cross-react with anti-GFP antibodies, making them difficult to use in a multicolor/multichannel...fluorophore-labeled antibodies with very specific reactivities making them more useful for multicolor imaging experiments...Sample preparation for these techniques sometimes makes it more difficult to detect protein tags using antibodies...Blog Rabies and neuronal tracing Retrograde AAV: Making the journey from axon to nucleus AAVs CREATEd for...

  27. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...their initial assumption. Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and.... Using computational observations, Koonin and Makarova found that Cas7-11 appeared to have derived from...generation came Prime editing — the ability to now make any small changes, including base edits, insertions...Özcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS. Programmable...

  28. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...larger target, such as a protein. Such flexibility makes aptamers ideal candidates for biosensors. Aptamer... of how they actually “report” as biosensors. Making aptamers glow The flexibility of aptamers means...rather than separate them (Fig. 1b). One can also make “signal-off” biosensors in which the aptamers fluoresce...decrease background in vivo. The nature of aptamers makes them excellent candidates for developing assays ...

  29. How to Be an Excellent Trainee

    Type
    Blog Post
    ...grumpiest of researchers can enjoy it at times! What makes it rewarding is when you can tell a trainee is understanding...to show up as your most prepared, can-do self and make the most of it! So, what can you do to build a ...reviewed above. Offer to help clean up and/or make necessary reagents for the experiments. Unlike in...need help with…now”. Ask for feedback so you can make adjustments. This could look like “What are some...

  30. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...pre-made viral preps. If you can’t (or don’t want to) make viral preps in your lab, there are custom viral ...verified!–your pooled library DNA, the next step is to make your virus. If the library happens to have a specific...vary with titer, cell type, and other factors, so make sure to put that aliquot to full use! Common conditions...chances that a cell doesn’t get infected at all. To make sure that every plasmid’s payload is adequately ...

  31. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...advent of CRISPR/Cas9 has made it easier than ever to make precise, targeted genome modifications. Cas9 has...many available through Addgene’s repository) and make it easy to find your perfect match. Choosing a ...xCas9 has a PAM requirement of NG, GAA, or GAT, making it a more flexible cutter with the added bonus ... You can achieve your precise edit without even making a break.  Base editors are dead Cas9 (dCas9) or...

  32. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...nowadays, we cannot make it a full month without a new, drug-resistant ‘superbug’ making headlines in one... develop survival mechanisms. Some microbes even make their own antibiotic compounds. In such a case, ...resistant bacteria to over-take the population and making them more difficult to treat in the future. Patients...

  33. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ... for talk is over and instead we must make real changes to make the system sustainable. For years, there...Department of Labor issued a Notice of Proposed Rulemaking, soliciting feedback by Sept 4th.  They proposed...opportunity to accept these recommendations, and make gradual adjustments towards this new level. Indeed...

  34. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...preference, some people like to make stricter gates while some like to make looser ones (Figure 3). A more...free to use SSC for a single cell gate. However, make sure to compare H and A (or W and A).   Live... type of stain whether a tighter or looser gate makes sense. Here, luckily, the vast majority of my cells...

  35. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...larger signal-to-noise ratios in two-photon imaging, making it ideal for video recordings of large populations...backbones designed right here at Addgene. Here's what makes these new backbones special: Strong expression:...Selectable and stable: A puromycin resistance gene makes stable cell line creation a breeze. Modular by design...recruitment and nicking, producing STITCHR. STITCHR can make a wide variety of genomic edits — single-base changes...

  36. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...yielding 1-10% knock-in. Read on to learn how you can make CRISPR mouse model generation easi-er! Generating...months. But certain models remain more difficult to make. For example, large insertions, like a fluorescent... site. If you are using the ivTRT method, try to make the first two bases of the left homology arm guanine...activity. To avoid re-cutting by Cas9 after editing, make sure that the repair template does not contain the...

  37. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...used for delivering your light stimulus. This can make or break your experiments!  First, closely read ....2021.778900 Mattis, J., Tye, K. M., Ferenczi, E. A., Ramakrishnan, C., O’Shea, D. J., Prakash, R., Gunaydin, L... B., Kadmon, J., Raja, C., Chibukhchyan, A., Ramakrishnan, C., Inoue, M., Shane, J. C., McKnight, D. J...science.aaw5202 Mattis, J., Tye, K. M., Ferenczi, E. A., Ramakrishnan, C., O’Shea, D. J., Prakash, R., Gunaydin, L...

  38. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ... of DNA as inert. It generally stays put, which makes it easy to locate in a genome. But there is a type...genomes of many other prokaryotes and eukaryotes, and make up ~45% of the human genome (Munoz-Lopez et al.,...considered replicative since every time they jump they make a copy of themselves. This increases the number ... by their nature, are mutagenic elements, which makes them a great tool for mutagenesis screens that detect...

  39. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...non-dividing cells, including stem cells. These properties make lentiviral vectors fantastic options for delivering...such as macrophages and microglia. This property makes the lentivirus an attractive choice for a gene transfer...the accessory factors in HIV positive cells could make the virus replication competent. The 3rd generation...transgene resulting in low inducibility and potentially making it difficult to interpret your results. This can...

  40. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...you get the idea. What if there were a way to just make one fusion protein that switches to any color or...experiment (Figure 3). And using self-labeling tags makes updating your labels easy! Instead of taking the...ligands is more expensive than letting the cells make a fluorescent protein for you. And some ligands ...Selecting Fluorescent Dyes and Ligands GCE4All: Making Genetic Code Expansion Accessible Addgene's Genetic...
Showing: 281 - 320 of 801 results