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We narrowed to 795 results for: Tec;

Showing: 281 - 320 of 795 results

  1. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... (reviewed in Shcherbakova et al., 2018). For detection of protein-protein interactions, we recommend ...Multiplexing and Optogenetics across Scales. Trends Biotechnol 36, 1230-1243 (2018). Shcherbakova, D.M. et al...Sivanesan, D. & Michnick, S.W. An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions...encoded calcium indicator for in vivo imaging. Nat Biotechnol 39, 368-377 (2021). Shcherbakova, D.M. Near-infrared... fluorescent protein for in vivo imaging. Nat Biotechnol 29, 757-761 (2011).   ...

  2. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...Physiology, Pathogenesis and Biotechnological Applications. InTech. https://doi.org/10.5772/67306 Koornneef...protein-protein interactions in proximity labeling techniques such as BioID (Roux et al., 2012) and APEX (Rhee...tools, such as gene manipulation and mutagenesis techniques, are available to the scientific community.  ... as a Model Organism and Its Application in Biotechnology. In Escherichia coli - Recent Advances on Physiology...

  3. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...RNA production can be detected by using a MS2 coat protein that specifically detects a specific RNA stem...instance, pleckstrin homology domains can be used to detect specific phosphoinositides (Varnai and Balla, 2008...Cas9 is a protein, a fusion with GFP allows for detection of genomic locations (Chen et al., 2013). GFP...fluorescent proteins (Chernov et al., 2017) may improve detection. Since longer wavelengths have better tissue penetration...

  4. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...Site directed mutagenesis is a highly versatile technique that can be used to introduce specific nucleotide...naturally occuring SNPs in a plasmid context). The technique is also highly relevant in this age of CRISPR;...plasmid digestion (blue). Digestion with enzyme “B” detects primer multimerization (purple) which is evident...~3%). Note that primer duplication will evade detection in the initial RFLP analysis simply because the...on the Addgene Blog Brush up on Your Cloning Techniques Learn More about Plasmids in Our Plasmids 101...

  5. Using AAV for Neuronal Tracing

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    Blog Post
    ...understanding the brain’s function is knowing its architecture, in particular the connections between different...nervous system With the advent of viral vector technology, new methods have been developed to study neuronal...consistent which may be caused by the diversity of techniques, species, and brain regions used. Evidence for... Gradinaru Lab at the California Institute of Technology generated novel AAVs (Chan et al., 2017) that...lower toxicity, higher specificity and reduced technical variability compared to chemical tracers. rAAV2...targets neonatal neurons and adult astrocytes. Nat Biotechnol 27, 59–65. PubMed PMID: 19098898. PubMed Central...

  6. 15 Hot Plasmids from 2017

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    Blog Post
    ...authors use PhoCl in combination with this caging technique to beautifully demonstrate light-dependent Gal4... base editors The popular CRISPR base editing technique uses dCas9/Cas9 nickase fused to a cytidine deaminase... PubMed PMID: 28585549 Kim Y, et al. Nature Biotechnology. 2017. PubMed PMID: 28191901 Listen to Our ...a fully sequenced genome, many applications in biotech, and is considered the most studied Gram-positive...vivo. Utilizing CRISPR interference (CRISPRi) technology, the arrayed knockdown library targets 289 genes...In Narayan et al., they used this now classic technique to elucidate the mechanism by which SOX2 acts ...

  7. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...pathways. Trends in Biotechnology, 33(2), 92–100. https://doi.org/10.1016/j.tibtech.2014.11.007...activate the pathway than traditional pharmacology techniques allow. Multiple photoswitches can be used in ...reading Abreu, N., & Levitz, J. (2020). Optogenetic Techniques for Manipulating and Sensing G Protein-Coupled...References Abreu, N., & Levitz, J. (2020). Optogenetic Techniques for Manipulating and Sensing G Protein-Coupled...

  8. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...their hot-off-the-presses papers, the awesome new technology they’re utilizing, and the tricks they’ve used...plasmids in your own lab. Summaries of the latest technologies and breakthrough research. Insights about ..., post-doctoral fellow, lab manager, research technician, or Principal Investigator. How-to guides for...a special focus on cloning and plasmid-based technologies. Commentary about scientific sharing and the...

  9. Podcast: Interview with Addgene's Founders

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    Blog Post
    ... Chapter 3 - Addgene as a leader in standardized tech transfer and the adoption of the UBMTA Chapter 4... and Diplomacy, with a focus on international technology management, from the Fletcher School at Tufts...investment banking analyst at Credit Suisse’s Technology Group in Palo Alto, CA.  Benjie Chen is a co-founder...and networks. Benjie was Addgene's Director of Technology for 7 years, and is now Chief Software Engineer...

  10. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...current forming membrane pores) transfection. These techniques are still used and efficiently transfer plasmid...not been tested for HIV.  If not using proper technique, there’s always a small chance that a researcher...transfection, CaCl2 transfection is a simple and cheap technique that should work well. Quickly after transfection...for virus titration: the p24 ELISA dosing method detects the expression of viral capsid antigen p24, the...Therapy, Dr. Ke Xu (Ed.), ISBN: 978-953-307-539-6, InTech. 4. Walter, W., and U. Stein. "Viral vectors for...

  11. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ... itself was relatively easy. Figure 4: Detection of edited BRAF genomic loci in Cas9-expressing...dsDNA) at basepair mismatches. To use this tool to detect a BRAF gene edit, simply PCR-amplify the BRAF locus...be in the lab. Based on the power of available techniques and how quick and easy it is to share information...Sullender M, Ebert BL, Xavier RJ, Root DE. Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026. 10.1038/nbt...Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. PubMed PMID...

  12. What's New in CRISPR - Winter 2018

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    Blog Post
    ...research.     Improved nucleic acid sequence detection Feng Zhang’s lab recently developed a platform...high-sensitivity enzymatic reporter unlocking) to detect single molecules of RNA or DNA. Now they have developed...developed this further to provide multiplexed detection, higher sensitivity, and more. Read the paper...and cleaves ssDNA which they have adapted for detecting SNPs. Learn more from this Addgene blog post...

  13. Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

    Type
    Blog Post
    ... modular, standardized, disciplined, and open techniques. Chapter 4: Research projects underway in the...Synthetic Biology. Wide adoption of synthetic biology techniques in biology research as can already be seen with...University of Cambridge before spending time in biotech screening direct interactions between drugs and...investigates bacteria and extremophiles for biotechnology and has been directly involved in supervising...

  14. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    ...backgrounds! Show off your favorite reagent, scientific technique, or plasmid repository at your next meeting.   ...blog topic to learn more about FRET and other techniques using FPs! Otherwise, check out our plasmid and... browse plasmids by field, organism, research technique, and more. Download the green DNA background. ...those backgrounds representative of your favorite technique? Our lab-themed background is a great fit for ...

  15. Addgene's Top Blog Posts from 2020

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    Blog Post
    ...depositors who quickly shared their reagents: detection methods, synthetic nanobodies, SARS-CoV-2 protein...reagent sharing even broader and began working with tech transfer offices to make reagents available to scientists...extracting RNA without a kit. 3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas by Shravanti Suresh...University detailed five methods for using CRISPR to detect SARS-CoV-2, including many with plasmids deposited...

  16. Typing CRISPR Systems

    Type
    Blog Post
    ...classification system for the array of CRISPR technologies. Learning the general principles of this classification...systems. Type III systems are unique, as they can technically target both RNA and DNA, though it is largely...the first taught when introducing CRISPR as a technology. There are three subtypes, A–C, with the beloved...Y. (2018). Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing. Cell ...Yoshimi, K., & Mashimo, T. (2022b). Genome editing technology and applications with the type I CRISPR system...

  17. Editor's Choice, September 2016

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    Blog Post
    ...this post even more useful to your colleagues. Technique: Probe Phage Genomes for Host Binding Proteins...editor’s choice posts this month. The first, "Technique: Probe Phage Genomes for Host Binding Proteins...from the University of Alberta and describes a technique you can use to find phage proteins that bind to...2) The post describes an ingenious but simple technique to screen for proteins that could be useful for...

  18. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ...are swarming to see what can be done with this technology to help patients. Dr. Stoffels and others in ...months compared to a year or greater with older technologies. Addgene is proud to be helping get CRISPR reagents..., the panelists agreed that, for example, the technology is already available today to cure sickle cell...really sophisticated”.     New to CRISPR/Cas9 Technology? Learn More: Addgene's CRISPR/Cas9 Guide History...

  19. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    ...It’s clear that CRISPR-Cas9 technology has really changed the game for anyone looking to quickly and ...question. Additionally, a concurrent Nature Biotechnology article describes the development of a mouse...accessible before are now accessible using this technology.” GeCKO library for genomic screens Using lentivirus... Castillo Velasco-Herrera M, Yusa K. Nature Biotechnology. 2013 Dec 23. (Article). Read more about CRISPRs...

  20. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...sensitive detection of the plant hormones strigolactones By Fabienne Furt Current methods used to detect strigolactones...CRISPR-based editing in organoid Biosensors for detecting strigolactones The CRISPR Corner New ready-to-...intensity, it provides a direct and quantitative detection of SLs. The authors also showed that the receptor-based...

  21. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    ...limitations by using gas vesicle reporters that are detectable by an inexpensive and widely available imaging...fluorescent or bioluminescent reporters usually only detects the signal of the reporter and fail to pinpoint...each ARG’s distinct ultrasound fingerprint can be detected. Bourdeau et al calls this method acoustic collapse... in relation to internal organs. The limit of detection for arg1 expressing ECN is 109 cells/ml, which...

  22. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...material for a ChIP-seq experiment can be a major technical hurdle.   ChIP-seq requires  cross-linking during...improvement and simplification of the original technique, specifically in terms of initial steps of cell...ChIP-seq, especially in situations when it is technically challenging to collect large numbers of cells...the CUT&RUN method, it seems likely that this technique will quickly become a standard method alongside...

  23. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    ...Snapgene generated maps provide improved feature detection with an easy to use interface. We regularly use... linear or circular sequence. Next, we use the “Detect Common Features” function to find and annotate ...s “Import primers from a list” function, we can detect all of the primers we have designed that will anneal...plasmid maps and sequences use the same feature detection software as the desktop version of Snapgene. When...

  24. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...purification tools (and can have secondary functions as detection aids.) When a single protein is expressed, with...from solution. MBP antibodies are available for detection and/or purification of MBP-tagged proteins. The...alternative to amylose resin and also provides a visual detection mechanism via applications such as western blot...of interest, especially if downstream antibody detection is desired… and yes, we have an antibody for that...

  25. Advice for Choosing a Research Project

    Type
    Blog Post
    ... be your critical experiments and why? What techniques and skills do you need to use to reach these ...address a topic in a mechanistic way using the technology available.” Choosing a research project “entails...entails good judgement with the current technology to identify a problem, asking what approach will I take... importance of combining “new discoveries or technologies increase chances that your project will be fresh...

  26. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...AAV viral preps to easily find and share useful technical data. These data focus on experimental design ... These questions are focused on injection and detection methods. For example, you can provide location...you wait until performing the experiment? What detection methods did you use for expression of AAV cargo...Considerations For Precise Neuronal Targeting Using AAV Technologies Using AAV for neuronal tracing Resources on...

  27. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...context of IHC, but the problem can impact other techniques like Western blots or immunocytochemistry (ICC... described here may be as effective for other techniques, but it is important to be aware that the issue...Flexible Mouse-On-Mouse Immunohistochemical Staining Technique Adaptable to Biotin-Free Reagents, Immunofluorescence..., Jacob DA (1990) A novel immunohistochemical technique for demonstration of specific binding of human...

  28. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...no post-PCR procedures. Sounds easy right? The technique relies on the basic ability of complementary basepairs...called TOPO-TA) cloning; however, the TOPO cloning technique has also be adapted for blunt end cloning. So ... DNA ends to facilitate replication. The TOPO technique specifically uses Vaccinia virus-isolated topoisomerase...Protocols Catch Up On Your Basic Molecular Biology Techniques Watch Our Protocol Videos ...

  29. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ...the chance to win some of the latest tools and technologies in life sciences research. The rare disease ...countries. “Over $600,000 worth of cutting-edge technologies were awarded to study 31 rare diseases,” said...ll combine induced pluripotent stem cell (iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit...those mutations, they now plan to use CRISPR-Cas9 technology to create cell lines and/or mouse models carrying...

  31. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...fluorescent ligands to the loci of interest. Dual color detection In order to label two loci simultaneously, Ma ...CRISPRainbow) to 3-fold with CRISPR-Sirius. Triple color detection With a third sgRNA containing alternating MS2 ...target site. Of these, 26 of the 46 loci gave a detectable signal in human U2OS cells. Based upon this, ...engineered sgRNAs using CRISPRainbow.” Nature Biotechnology 34 (2016): 528-530. PubMed PMID: 27088723 PubMed...

  32. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...experiments in this publication were performed using a technique called nucleofection, which introduces functional...2016, and Kim et al 2014) have shown that this technique supports extremely high frequencies of genome ...experiments to try nucleofection as (in our hands) this technique supports gene disruption or gene replacement frequencies...CRISPR-Cas9 using asymmetric donor DNA." Nature biotechnology (2016). PubMed PMID: 26789497.  2. Lin, Steven...

  33. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...analysis blog post. Colony PCR Colony PCR can detect the presence or absence of DNA using lysed bacterial...plasmid. In this case, you’d be using colony PCR to detect your insert, either by using primers that are specific...time-intensive than Sanger sequencing or the other techniques mentioned above. In molecular biology, cloning...By using a variety of screening and selection techniques, you can increase the chances of finding the ...

  34. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...revolutionary physical approaches. However, with each new technical achievement, the complexity of the device increases...PhD, I have been convinced that most microscopy techniques can be replicated with commercially available...available tools and components. Rapid prototyping techniques such as 3D printing, on-demand CNC milling, and... and PCB manufacturing. These newly developed techniques are the key enablers for making assemblies open-source...

  35. A Deep Dive into BioBuilder

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    Blog Post
    ...inspire them and also keep up with all the new biotechnology.” These are the types of inquiries that routinely...experiential learning. “Students may forget the technical details they hear in their classes,” she said,...module currently under development comes from the Caltech lab of Professor Richard Murray. Murray’s lab uses... DNA circuits and logic gates. This BioBuilder-Caltech collaboration has resulted in a new BioBuilder ...

  36. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post
    ...at each locus, as well as large deletions. They detected fragment deletion with 4-20% frequency at the ...you have any questions or thoughts about this technology, leave them in the comments below.  References...plasmids from this publication at Addgene Other Techniques for gRNA Multiplexing Multiplexed and programmable... Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Jun;32(6):569-76. doi: 10.1038/nbt.2908...

  37. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ... but this skill is not just about reacting to technical issues. This skill also comes into play while ...encounter. As you spend time in a lab learning techniques and taking on independent projects, you will ...solutions. Ultimately, problem solving is less about technical know-how and more about how you approach new challenges...important. Paired with your other skills, both technical and transferable (i.e. teamwork and time management...

  38. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    .... We also opened up applications to research technicians and undergraduate researchers, in addition to...Abhi Aggarwal is a postbaccalaureate research technician in Kaspar Podgorski’s lab at Janelia Research...variants of iGluSnFR3 in vivo and have used it to detect glutamate release from hundreds of synapses at ...believe that improved biosensors or biosensors that detect new redox metabolites are vital for researchers...

  39. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...innovations” stimulates excellence in science and technology by integrating sex and gender analysis into research...anything unique to male stem cells. Nor did they detect important differences in function between male ...international expert on gender in science and technology and currently directs the EU/US Gendered Innovations...Nations on the topic of “Gender, Science, and Technology” in 2011 and again in 2014. Resources on the ...

  40. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...to your cloning efforts. Golden Gate cloning technology relies on Type IIS restriction enzymes, first...Voytas groups described a new Golden Gate-based technology for genome editing which allowed for the ordered...efficiently in just a few steps. More recently, CRISPR technology has adapted Golden Gate cloning for inserting...Expression of gRNAs Choosing a Molecular Cloning Technique  ...
Showing: 281 - 320 of 795 results