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  2. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...Yoshimi, K., & Mashimo, T. (2022). Genome editing technology and applications with the type I CRISPR system...for genome engineering in human cells. Nature Biotechnology, 37(12), 1471–1477. https://doi.org/10.1038/...

  3. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...physical models of proteins using 3D printing technology. The tagline for our company is “…where molecules...effective this model was in explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new...

  5. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...control protein expression for experimental, biotechnological, and medical applications. Researches can ...desired protein. Despite being a rather nascent technology, plant cells have been used to express a wide...

  7. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...pipettor is set to 200 ul, wiped down with 70% ethanol, and a box of sterile truncated pipet tips (USA...where it immediately spreads out to form a firm monolayer. The cell clones - easily identified from the ...

  11. AAVs in Retinal Gene Therapy

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    Blog Post
    ... I quickly became fascinated by this emerging technology and its potential for the treatment of some genetic...research is being done to further improve the technology: engineering of new capsid variants, designing...

  12. Getting to Know Expansion Microscopy

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    Blog Post
    ...anchoring strategy for expansion microscopy. Nat Biotechnol 1–12. https://doi.org/10.1038/s41587-022-01546...standard fluorescent proteins and antibodies. Nat Biotechnol 34:987–992. https://doi.org/10.1038/nbt.3625  ...

  14. DNA Purification Without a Kit

    Type
    Blog Post
    ...RNase to remove RNA contamination and you can use a phenol chloroform extraction to remove remaining proteins...of four times before a wash with ddH2O and then ethanol. After regeneration, trace amounts of DNA (3 femtograms...

  15. MXS Chaining

    Type
    Blog Post
    ...proteins and the availability of powerful imaging technologies, the combination of multiple fluorescent protein...High Throughput Cloning Strategies Method Technology Pros Cons References Golden Gate Type II restriction...

  16. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post
    ... chromophore to form with an indole rather than phenol component (cyan derivatives) Y66H Blue-shifts... many other uses currently exist, and new GFP technology is constantly being developed! Fusion tagging...

  17. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ... execute the proposal and the tools/resources/technology exist to solve the problem. What will you have...received his PhD in Molecular Microbiology and Immunology from Oregon Health Sciences University, did his...

  18. Career Insights: Technical Support Specialist

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    Blog Post
    ...necessary), there are opportunities to work for biotechnology companies and help other researchers use the...experience and scientific knowledge they require. Biotechnology companies vary to what extent they hire experts...

  20. Neuronal labeling with Spaghetti Monster

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    Blog Post
    ...also be used with the most cutting-edge imaging technologies such as array tomography, super-resolution STORM...W. Davidson. "Advances in fluorescent protein technology." Journal of cell science 120.24 (2007): 4247...

  21. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ..., Methylophilus methyltrophus, Methylococcus methanolicus, Methylosinus trichosporium, Myxococcus xanthus...protein expression across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https:/...

  22. Lab to Office Culture Shock

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    Blog Post
    ... my work. The meetings often are the work. Will Arnold, one of the newer members of our Scientist team...and for modeling in the picture above), and Will Arnold for their ideas and tips! Additional resources ...

  24. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ... be used in some in vivo experiments and in biotechnology and therapeutics (Mauro and Chappell 2014).  ... heterologous protein expression. Trends in Biotechnology 22:346–353 . https://doi.org/10.1016/j.tibtech...

  25. Overcoming the AAV Size Limitation for CRISPR Delivery

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    Blog Post
    ...adeno-associated viral (AAV) vector toolbox. Biotechnology Journal 9:1402–1412 . https://doi.org/10.1002...in the mammalian brain using CRISPR-Cas9. Nat Biotechnol 33:102–106 . https://doi.org/10.1038/nbt.3055...

  26. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol 34:863–868 . https://doi.org/10.1038/nbt.3609...CRISPR-Cas Cpf1 nucleases in human cells. Nat Biotechnol 34:869–874 . https://doi.org/10.1038/nbt.3620...

  27. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...Fluorescence resonance energy transfer (FRET) technology has been studied and used in assays for over ...PMC4441765. 4. Guo, Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014...

  28. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...discovery through the creation of innovative technologies. To date his group has develop several popular...genome editing and transcription modulation. Nat Biotechnol 33:139–142 . https://doi.org/10.1038/nbt.3149...

  29. The Challenges of Cell Culture

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    Blog Post
    ...tool used in the fields of oncology, virology, immunology, microbiology, and pharmacology (many of which...stance on the matter. Funding bodies such as the Biotechnology and Biological Sciences Research Council (BBSRC...

  30. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...ribonucleoprotein complexes in vivo. Nature Biotechnology, 10.1038/s41587-023-02078-y. doi: https://doi.org...DNA sequences with twin prime editing. Nature Biotechnology, 40(5), 731–740. doi: https://doi.org/10.1038...

  31. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...Thomason et al., 2014). In recent years, CRISPR-Cas9 technology has also aided in the creation of scarless mutations...genomes using CRISPR-Cas systems." Nature biotechnology31.3 (2013): 233. PubMed PMID: 23360965. PubMed...

  33. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...searches. I've updated chapters to reflect new technologies, i.e. video interviews. For point of reference...received his PhD in Molecular Microbiology and Immunology from Oregon Health Sciences University, did his...

  34. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...modifications feasible and even easy. This targeted-break technology coupled with HDR is a powerful site-specific ...principles driving CRISPRs and genome editing technology. Links: Zhang Lab’s CRISPR FAQs CRISPR forum...

  35. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... as a Model Organism and Its Application in Biotechnology. In Escherichia coli - Recent Advances on Physiology...Physiology, Pathogenesis and Biotechnological Applications. InTech. https://doi.org/10.5772/67306 Koornneef...

  37. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...times, have led researchers to turn to other technologies like CRISPR. Figure 1: An overview of...CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Kellner...

  38. Antibodies 101: Multiplex Immunofluorescence

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    Blog Post
    ...formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed cell samples, or other...monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife, 8, e43322. https://doi.org...

  39. Finding Your Perfect Job After University

    Type
    Blog Post
    ...trying to get hands-on lab experience is that the technology is so advanced that most of the procedures in...own challenges. I’ve had to become skilled with technology like Skype in order to have meetings between ...

  40. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...proteins with small molecules in vivo. Nature Biotechnology, 21(1), Article 1. https://doi.org/10.1038/nbt765... V. (2008). HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis. ACS Chemical...
Showing: 281 - 320 of 545 results