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We narrowed to 784 results for: SCR;

Showing: 351 - 400 of 784 results

  1. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...mutagenesis to extend at different pH values. Using a screen that takes advantage of the fact that coiled R-...both lower and higher pH. We initially used this screen to find R body mutants that couldn’t extend at ...

  2. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...mammalian plasmid components, it may be useful to describe the means of introducing genetic material (such...the plasmid in the same way. Selection methods, described below, are typically employed when creating stable...

  4. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...forward genetics harnesses this at a large scale by screening diverse libraries of genetic variants. Several... integration of oligonucleotide libraries of a discrete size at the target site. The use of such libraries...

  5. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...Pol, the last component, contains the reverse transcriptase and integrase components to convert the cargo...the primary viral polymerase. E4/E1A activate transcription of other relevant viral genes (including the...

  6. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...single-stranded RNA viruses that use reverse transcriptase to synthesize complementary DNA copies of their... such as a heterologous envelope or a post-transcriptional regulatory element can be used for either type...

  7. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...trillion connections (synapses.)  In this post we’ll describe the AAV retrograde serotype (AAVrg) and how it..., 2022) and substantia nigra (Tervo, 2016). Discrepancies in transduction efficiency can have a major ...

  10. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...better substrates for ligation. Although we do not describe these steps here, the processing of DNA ends tends...Genome-Wide Control for Large-Scale Functional Screens        ...

  13. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...video_player "embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button...', style='' %} Resources on the Addgene blog Subscribe to the Addgene blog Read our viral vector blog...

  15. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...Another similarity to Cas12a is that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged... the genome. Cas13, similar to Cas14, also indiscriminately cleaves ssRNA when engaged at its RNA target...

  16. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...molecular biology experiments was in the job description, but this skill is not just about reacting to...research non-academic careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined...

  17. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...figure out how to optimize protocols for in vivo screening with lentiviral pooled shRNA libraries. Later,... Blog Learn about the Sabatini and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource...

  18. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ... fragments, these problems can be overcome by screening a higher number of potential clones. Golden Gate...In early 2011, the Bogdanove and Voytas groups described a new Golden Gate-based technology for genome ...

  19. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...Cre-dependent gene expression – placing a series of transcriptional and translational termination features with... Combining this with some of the loxP methods described above, a genetic modification can be restricted...

  20. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...method seems simple, I found it hard to remember to describe all four parts of a STAR answer once the interview...hard during this talk since you’ll be sharing your screen and won’t be able to see who’s asking a question...

  22. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...of citations per year and the number of other manuscripts that heavily cite the paper are displayed. You...the context of these citations. The number of manuscripts that cite the background, methods, or results...

  23. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...Resonance Energy Transfer (FRET) was originally described by Theodor Förster in 1948 as a variation of the...FRET probe, try searching PubMed for articles describing the FRET tool that you are looking for and check...

  24. Hot Plasmids February 2024

    Type
    Blog Post
    ...authors used a multispecies approach to generate and screen a library of AAV9 variants (Chuapoco et al., 2023... Figure 3: Overview of library generation and screening that led to identification and characterization...

  26. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...cloning, the Haynes lab’s Protocols.io protocol describes an elegant system that allows for the assembly...editing assemblies are sensor-actuators, either transcriptional regulators or chromatin-modifying enzymes. ...

  27. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...forward as quickly as possible and submitted our manuscript to the journal Cell just six weeks later. The...Asian-, African- and American-lineages). Strain discrimination is especially important in the current outbreak...

  29. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants that inherited an edited...for its improved kinetics and responsivity in screening ASAP3 variants. B) Electrical and optical recordings...

  30. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...sense, models can be both useful, and dangerous. Subscribe to CRISPR Blog Posts How do educators and students...reaction to this model on his blog. Or, for a description of how effective this model was in explaining...

  31. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...while seated or with your poster on your computer screen than it is to pretend you’re standing at the session...poster board well ahead of time ensures you aren’t scrambling to tack up your poster seconds before the session...

  32. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...component as well as a small molecule which we will describe in more detail below. Types of Voltage Indicators...membrane, a concern with several of the previously described systems. Several adaptations of this method have...

  33. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...used to quantitate RNA levels using reverse transcription-qPCR (RT-qPCR). The first step of this process...requires RNA to be converted to cDNA using reverse transcription and the cDNA is subsequently quantified by qPCR...

  34. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...processes. To create the antibody toolbox, they screened antibody candidates using a synthetic human scFV...cross-reactivity, antibody candidates underwent affinity screening to select for slow off-rate binders. The best ...

  35. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...After this initial application, Stelzer’s group described the single-plane or selective-plane illumination...optical sectioning of a specimen is based on discriminating the out-of-focus reflected light by using a...

  38. DNA Purification Without a Kit

    Type
    Blog Post
    ...plasmid purification and DNA gel extraction method described in Kim and Morrison is nearly identical to that...Additionally, while commercial plasmid prep kits come in discreet sizes (mini, midi, maxi, giga) which requires ...

  39. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...HCab antibodies from its serum, and meticulously screening, optimizing, and characterizing several anti-mouse...review. If you just want to use the nanobodies described in Pleiner et al, the key steps for generating...

  40. MXS Chaining

    Type
    Blog Post
    ...expression) and a polyA signal (to terminate transcription) and the four constructs were then combined ...their 6-mer sequences, are rarely found in the transcriptomes of these species and can often be used to clone...

  42. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...standards (clear and detailed methods and image descriptions, a minimum n = 2, and well labeled high- and...’ results is available not through published manuscripts, but through word of mouth or old lab notebooks...

  43. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA...act as a feedback mechanism to reduce further transcription. Replication is also regulated by the balance...

  44. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...s lab performed a series of positive selection screens in bacteria to identify mutants of S. pyogenes ...sequences (Kleinstiver et al. 2015). From these screens, they identified four novel SpCas9 variants with...

  46. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...editing analysis Once the analysis is complete, a new screen appears with a graphical representation of the ...Data button on the bottom right of the analysis screen. ICE analysis details Contributions   The Contributions...

  48. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ..., and a PhD is not always necessary. The job description and the candidate requirements (and maybe also...customers, internal resources and databases are scrutinized, and this information is enriched with your technical...

  49. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...enrichment (SELEX), some 1010 oligonucleotides could be screened for their affinity to a wide range of non-nucleotide... as the aptamer is bound to a protein fluor. Transcribing and translating a protein fluorophore also takes...

  50. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...synthesis companies, such as IDT, Genewiz and GenScript, offer tools to help with these decisions.  So...ATUM DNA2.0  IDT Synthetic gene design made easy GenScript- GenSmart Codon Optimization References Athey...
Showing: 351 - 400 of 784 results