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Showing: 351 - 400 of 535 results

  1. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...challenging to compete in today’s tough academic or industry job market for biology PhD’s. I was facing the...in 2007. Nikolai trained as a post-doc at the Biochemistry Department at the University of Manchester as...receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois Institute of Technology in...

  2. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...can be easily modified in order to facilitate its entry into the target cells (Beatty et al., 2012). These...Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far) or for cancer...therapy: Current state and clinical perspectives. Biochemistry Moscow 81:700–708 . https://doi.org/10.1134/...

  3. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...human genes and are recommended for use in immunohistochemistry (Figure 5). Anti-MAPT [AT8] specifically...Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant..., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org...

  4. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...generates pores in the cell membrane, allowing for entry of the RNP into the cytoplasm. In addition to the...forms pores in the nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated...electroporation of zygotes." Journal of Biological Chemistry (2016): jbc-M116. PubMed PMID: 27151215. PubMed...

  5. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...machine learning to solve difficult problems in chemistry and chemical biology.       David R. Liu is Director... Faculty at the Broad Institute; Professor of Chemistry and Chemical Biology at Harvard University; and...Institute Investigator. Liu’s research integrates chemistry and evolution to illuminate biology and enable...

  6. Hot Plasmids - November 2023

    Type
    Blog Post
    ...label proteins with an alkyne group for click chemistry reactions instead of biotin — a valuable development..., then the other, a dual-enrichment and mass spectrometry workflow identifies proteins bearing both labels...their initial tests with thick slice (70 μm) immunohistochemistry (IHC) (Figure 5). PSD-95 is a scaffolding...

  7. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...those conversations, reading online healthcare industry publications like FiercePharma, FierceBiotech,...about the trends and workings of the healthcare industry. Finally, on-campus resources like career offices... both provide up-to-date information about the industry, recruiting processes, and can connect you to ...

  8. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... chemically pre-treating the cells to allow for entry of the antibodies. Here, a two-step process is employed.... The main applications of dye stains in flow cytometry are cell proliferation and cell viability. Unlike...101: Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...

  9. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... unconjugated and recommended for use in flow cytometry. Based on the established applications of the ...will also work in immunoprecipitation and immunohistochemistry. Table 1: IPI antibodies  Antibody ...Addgene's Antibody Guide Antibodies 101: Flow Cytometry Antibodies 101: Introduction to Antibodies...

  10. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...more stable yet brighter furimazine by Promega’s Chemistry group is a good story (1). From a deep sea shrimp...successfully infected/expressing cells by flow cytometry. The isolated cells were injected into mice and...enable sensitive in vivo optical imaging and flow cytometry for monitoring tumorigenesis. Cancer Res. 75, ...

  11. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... populations of synapses and deep-brain fiber photometry.  Figure 1: Characterization of iGluSnFR4...immunoprecipitation (IP), and tandem mass tag (TMT) mass spectrometry (MS) in HCT116 cells (colorectal cancer). The...isolated and identified with tools like mass spectrometry, providing a snapshot of interacting proteins...

  12. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ... contributed by guest blogger Søren Hough, a Biochemistry PhD candidate at the University of Cambridge...standardized validation techniques for academia, industry, and especially the clinic. Popular validation...contributions to this work. Søren Hough is a Biochemistry PhD candidate in the laboratory of Prof Steve...

  13. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...Although only wt hCas9 entry vectors are supplied with the kit, you can clone your own entry vectors containing...expressing wt humanized Cas9 and up to three gRNAs. Entry vectors containing the U6 promoter and the gRNA ...

  14. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... et. al. Science . 1996 272:263-267. PubMed . Asymmetry in the assembly of the RNAi enzyme complex. . ...Bioanalytical catalog # AB01200-02000, LB contains: 10g tryptone 5g yeast extract 10g sodium chloride 15g agar ...

  16. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    ...serotype, promoter, or tool type. Drilling into any entry will reveal additional data including the subject...injection before performing the experiment. Every entry has an accompanying image so that you can see for...

  17. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ... if an export license is not required for your country after checkout, and the processing fee will be ...licenses is available through the US Bureau of Industry and Security. If you have any questions about ...

  19. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ...date, material, requesting PI & organization, and country. This information can be of personal interest to...horizontal bar chart) Number of requests per country (as dot plot) Number of requests per year (as...

  20. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ... The Tarling-Vallim Lab’s first place-winning entry doubled as their lab’s decoration competition, and...snowflakes and viruses both exhibit similar forms of symmetry. 🎶Walking in a Winter Wonderlab🎶 We decided...

  21. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ... data, serve society and patients, and respect industry concerns.” 5)      In the aftermath of the Supreme...factor, study topic, and the corresponding author's country of origin, were taken into account. 7)     Science...

  24. An Addgene Summer Internship

    Type
    Blog Post
    ...was unsure whether or not I wanted to major in biochemistry. There, I had exchanged contact with Amy C.,...internship at Addgene. They plan on majoring in biochemistry in college and play the drum set as their personal...

  26. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...discernible paths. The first leads into the world of industry. Along this road, science communication graduates...ranged from wildlife conservation to abortion law to poetry. A research discipline in its own right, science...

  27. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...neuroscience and is available to both academic and industry scientists.  The collection of antibodies available...2019), validated in both western blot and immunohistochemistry. Additionally, many of the hybridomas from...

  28. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...can directly interfere with a seamless jump into industry.” In his article On Leaving Academe, Terran Lane...budget constraints Academic science is much like industry but the “product” is publications—the pressure...

  29. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...tags are frequently used for western blot, flow cytometry, and immunofluorescence . However, protein purification...tag is ideal for live cell imaging, FRET, flow cytometry, or immunofluorescence where an antibody can’t...

  31. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...had a bachelor’s degree or a PhD, majored in Biochemistry or International Finance. A book club could ...lapsed. Baker’s novel reignited my appreciation of poetry, and I’ve been reading a poem a day for the last...

  32. The MTA at Addgene

    Type
    Blog Post
    ...have the option of distributing under Addgene’s Industry MTA (IMTA), which allows the plasmids to be distributed...distributed for research use only to industry-based scientists. Some organizations choose to share their...

  33. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...no longer just for those working in academia or industry. At BosLab, we identify ourselves as part of a...creations that may not be pursued in an academic or industry lab. Mrina Nikrad, Ph.D., is an expert in microbial...

  34. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...in 2007. Nikolai trained as a post-doc at the Biochemistry Department at the University of Manchester as...receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois Institute of Technology in...

  35. RNA Extraction Without A Kit

    Type
    Blog Post
    ... acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299. https://doi.org/10.1021/bi00591a005...Thiocyanate–Phenol–Chloroform Extraction. Analytical Biochemistry 162:156–159. https://doi.org/10.1016/0003-2697...

  39. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ... has a compulsory internship to be taken in an industry, regulatory, or clinical environment. It remains...there for STEM graduates and postdocs in academia, industry and other areas of employment? How many graduates...

  40. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...ligated into a vector containing those overhangs. Entry DNA overhangs may be present in the original plasmid... 30-minute reaction. The destination vector and entry vector(s) are placed in a single tube containing...

  41. Engaging with science and society at pgEd

    Type
    Blog Post
    ...meetings of experts and leaders from academia, industry, community, education and government; advising...academic venues such as scientific, educational or industry conferences, as well as schools, community events...

  42. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...transmits retrogradely to first order neurons, via entry mediated by the G protein/G-protein receptor. Once...powerful tool that can be used to reveal brain circuitry, and continue to contribute answers to the complex...

  44. Hot Plasmids February 2024

    Type
    Blog Post
    ... toxicity from electroporation or poor cellular entry with other transfection methods. The Junwei Shi ...human cell lines and primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely...

  45. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ... “cited by” and references sections of a PubMed entry are great places to look for more articles on your...contain your search term. When you view a paper’s entry, the number of citations that paper has is displayed...

  47. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    .... 1: A schematic representation of Sybr Green chemistry. Blue arrows represent fluorescent signaling in... into thousands of PCR reactions using Taqman chemistry (see image, left, of droplet immersion technique...

  49. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...gene of interest, but after an internal ribosome entry site (IRES). This is oftentimes used in conjuction...Fluorescence-activated cell sorting (FACS): This is a type of flow cytometry that separates mixtures of cells into distinct...

  50. The Challenges of Cell Culture

    Type
    Blog Post
    ...that can impede collaborations, especially with industry. Today we also have the appropriate tools to verify...the European distributor for ATCC. He studied biochemistry at Warwick University, gaining extensive cell...
Showing: 351 - 400 of 535 results