We narrowed to 539 results for: try;

...challenging to compete in today’s tough academic or industry job market for biology PhD’s. I was facing the...in 2007. Nikolai trained as a post-doc at the Biochemistry Department at the University of Manchester as...receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois Institute of Technology in...

...human genes and are recommended for use in immunohistochemistry (Figure 5). Anti-MAPT [AT8] specifically...Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant..., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org...

...can be easily modified in order to facilitate its entry into the target cells (Beatty et al., 2012). These...Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far) or for cancer...therapy: Current state and clinical perspectives. Biochemistry Moscow 81:700–708 . https://doi.org/10.1134/...

...generates pores in the cell membrane, allowing for entry of the RNP into the cytoplasm. In addition to the...forms pores in the nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated...electroporation of zygotes." Journal of Biological Chemistry (2016): jbc-M116. PubMed PMID: 27151215. PubMed...

...machine learning to solve difficult problems in chemistry and chemical biology.       David R. Liu is Director... Faculty at the Broad Institute; Professor of Chemistry and Chemical Biology at Harvard University; and...Institute Investigator. Liu’s research integrates chemistry and evolution to illuminate biology and enable...

...label proteins with an alkyne group for click chemistry reactions instead of biotin — a valuable development..., then the other, a dual-enrichment and mass spectrometry workflow identifies proteins bearing both labels...their initial tests with thick slice (70 μm) immunohistochemistry (IHC) (Figure 5). PSD-95 is a scaffolding...

...those conversations, reading online healthcare industry publications like FiercePharma, FierceBiotech,...about the trends and workings of the healthcare industry. Finally, on-campus resources like career offices... both provide up-to-date information about the industry, recruiting processes, and can connect you to ...

... chemically pre-treating the cells to allow for entry of the antibodies. Here, a two-step process is employed.... The main applications of dye stains in flow cytometry are cell proliferation and cell viability. Unlike...101: Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...

... unconjugated and recommended for use in flow cytometry. Based on the established applications of the ...will also work in immunoprecipitation and immunohistochemistry. Table 1: IPI antibodies  Antibody ...Addgene's Antibody Guide Antibodies 101: Flow Cytometry Antibodies 101: Introduction to Antibodies...

... populations of synapses and deep-brain fiber photometry.  Figure 1: Characterization of iGluSnFR4...immunoprecipitation (IP), and tandem mass tag (TMT) mass spectrometry (MS) in HCT116 cells (colorectal cancer). The...isolated and identified with tools like mass spectrometry, providing a snapshot of interacting proteins...

...more stable yet brighter furimazine by Promega’s Chemistry group is a good story (1). From a deep sea shrimp...successfully infected/expressing cells by flow cytometry. The isolated cells were injected into mice and...enable sensitive in vivo optical imaging and flow cytometry for monitoring tumorigenesis. Cancer Res. 75, ...

... contributed by guest blogger Søren Hough, a Biochemistry PhD candidate at the University of Cambridge...standardized validation techniques for academia, industry, and especially the clinic. Popular validation...contributions to this work. Søren Hough is a Biochemistry PhD candidate in the laboratory of Prof Steve...

... et. al. Science . 1996 272:263-267. PubMed . Asymmetry in the assembly of the RNAi enzyme complex. . ...Bioanalytical catalog # AB01200-02000, LB contains: 10g tryptone 5g yeast extract 10g sodium chloride 15g agar ...

...Although only wt hCas9 entry vectors are supplied with the kit, you can clone your own entry vectors containing...expressing wt humanized Cas9 and up to three gRNAs. Entry vectors containing the U6 promoter and the gRNA ...

...control exceptions were made by the US Bureau of Industry and Security, pursuant to the international controls...-date information on shipping plasmids to your country. Curious about how rabies plasmids are used in ...

...serotype, promoter, or tool type. Drilling into any entry will reveal additional data including the subject...injection before performing the experiment. Every entry has an accompanying image so that you can see for...

... if an export license is not required for your country after checkout, and the processing fee will be ...licenses is available through the US Bureau of Industry and Security. If you have any questions about ...

... the overall plasmid selection will be open to industry scientists. This collection is highly diverse ...controlled through next generation sequencing and spectrophotometry to test purity (260/280 nm and 260/230 nm ...

...date, material, requesting PI & organization, and country. This information can be of personal interest to...horizontal bar chart) Number of requests per country (as dot plot) Number of requests per year (as...

... The Tarling-Vallim Lab’s first place-winning entry doubled as their lab’s decoration competition, and...snowflakes and viruses both exhibit similar forms of symmetry. 🎶Walking in a Winter Wonderlab🎶 We decided...

... data, serve society and patients, and respect industry concerns.” 5)      In the aftermath of the Supreme...factor, study topic, and the corresponding author's country of origin, were taken into account. 7)     Science...

...There are also different regulations from country to country when it comes to administering phage therapy...

...was unsure whether or not I wanted to major in biochemistry. There, I had exchanged contact with Amy C.,...internship at Addgene. They plan on majoring in biochemistry in college and play the drum set as their personal...

...-Niang (above, left) is an Full Professor in Biochemistry with a background in virology who has worked...Jun 15;134:1-17. PubMed PMID: 27984002. 2. Bilan, Dmitry S. et al. “HyPer-3: A Genetically Encoded H2O2 ...

...can directly interfere with a seamless jump into industry.” In his article On Leaving Academe, Terran Lane...budget constraints Academic science is much like industry but the “product” is publications—the pressure...

...tags are frequently used for western blot, flow cytometry, and immunofluorescence . However, protein purification...tag is ideal for live cell imaging, FRET, flow cytometry, or immunofluorescence where an antibody can’t...

...discernible paths. The first leads into the world of industry. Along this road, science communication graduates...ranged from wildlife conservation to abortion law to poetry. A research discipline in its own right, science...

...neuroscience and is available to both academic and industry scientists.  The collection of antibodies available...2019), validated in both western blot and immunohistochemistry. Additionally, many of the hybridomas from...

...Previous work by Stoneham et al. demonstrated that the entry of AAV2 into target cells is blocked following sucrose...adeno-associated virus/phage." Journal of Biological Chemistry (2012): jbc-M112. PubMed PMID: 22915587. PubMed...

...have the option of distributing under Addgene’s Industry MTA (IMTA), which allows the plasmids to be distributed...distributed for research use only to industry-based scientists. Some organizations choose to share their...

...together in chemistry to see what happens, but there isn’t really an equivalent of a “chemistry kit” for ...

...no longer just for those working in academia or industry. At BosLab, we identify ourselves as part of a...creations that may not be pursued in an academic or industry lab. Mrina Nikrad, Ph.D., is an expert in microbial...

...had a bachelor’s degree or a PhD, majored in Biochemistry or International Finance. A book club could ...lapsed. Baker’s novel reignited my appreciation of poetry, and I’ve been reading a poem a day for the last...

...in 2007. Nikolai trained as a post-doc at the Biochemistry Department at the University of Manchester as...receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois Institute of Technology in...

... anyway, including the entire faculty of the biochemistry department at the University of Massachusetts...changed since the injunction”. Postdocs around the country have now begun to speak up on this issue. These...

... acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299. https://doi.org/10.1021/bi00591a005...Thiocyanate–Phenol–Chloroform Extraction. Analytical Biochemistry 162:156–159. https://doi.org/10.1016/0003-2697...

...Physiology. Her research focuses on the neural circuitry involved in mechanical allodynia, a condition ... light activation and CNO silencing. (D) Immunohistochemistry verification of the dual virus expression...

...directly visualized with fluorescence or immunohistochemistry methods, these proteins are routinely fused... also be identified using antibodies and immunohistochemistry techniques. Thus, these proteins provide...

... has a compulsory internship to be taken in an industry, regulatory, or clinical environment. It remains...there for STEM graduates and postdocs in academia, industry and other areas of employment? How many graduates...

...ligated into a vector containing those overhangs. Entry DNA overhangs may be present in the original plasmid... 30-minute reaction. The destination vector and entry vector(s) are placed in a single tube containing...

...meetings of experts and leaders from academia, industry, community, education and government; advising...academic venues such as scientific, educational or industry conferences, as well as schools, community events...

...transmits retrogradely to first order neurons, via entry mediated by the G protein/G-protein receptor. Once...powerful tool that can be used to reveal brain circuitry, and continue to contribute answers to the complex...

...identifying PPIs, such as affinity purification mass spectrometry (AP-MS), lack the ability to specifically identify... purified via streptavidin pulldown. Following trypsin digest, PPIs are identified via mass spec analysis...

... “cited by” and references sections of a PubMed entry are great places to look for more articles on your...contain your search term. When you view a paper’s entry, the number of citations that paper has is displayed...

... toxicity from electroporation or poor cellular entry with other transfection methods. The Junwei Shi ...human cell lines and primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely...

...also culminated in a joint American Society for Biochemistry and Molecular Biology/ FoR Hack Day during which...FLSA) which mandates that all workers across the country either be paid more than $47,476 or be eligible...

.... 1: A schematic representation of Sybr Green chemistry. Blue arrows represent fluorescent signaling in... into thousands of PCR reactions using Taqman chemistry (see image, left, of droplet immersion technique...

...Pubmed. P.A Santi. (2011). Journal of Histochemistry & Cytochemistry, 59, 129-138. Pubmed. J. Huisken, and...

...gene of interest, but after an internal ribosome entry site (IRES). This is oftentimes used in conjuction...Fluorescence-activated cell sorting (FACS): This is a type of flow cytometry that separates mixtures of cells into distinct...

...that can impede collaborations, especially with industry. Today we also have the appropriate tools to verify...the European distributor for ATCC. He studied biochemistry at Warwick University, gaining extensive cell...