AAV Production in HEK293 Cells
Type
Protocol
...55 mL of heat-inactivated FBS and 5 mL of glutaGRO 11 mL of 200 mM L-alanyl-L-glutamine. Store at 4 °C....
We narrowed to 169 results for: 11
-
-
A Guide to Getting Started in Undergrad Research
TypeBlog Post...Students. Social Psychological and Personality Science 11:647–657 . https://doi.org/10.1177/1948550619882032... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post...specificity of GFAP transgenes. Neurochemical Research, 29(11 SPEC. ISS.), 2075–2093. https://doi.org/10.1007/s11064... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...." Current protocols in pharmacology 75.1 (2016): 11-19. PubMed PMID: 27960028. Van Duyne, Gregory D. ... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog Post...chromosome engineering in Escherichia coli. PNAS 2000 97 (11) 5978-5983; May 16, 2000, doi:10.1073/pnas.100127597... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...biotechnology 29.9 (2011): 816-823. PubMed PMID: 21822255. 11. Wang, Xiaoling, et al. "Unbiased detection of off-target... -
Luciferase Plasmid Collection
TypeCollection...split into Large (LgBiT; 18 kDa) and Small (SmBiT; 11 amino acids) fragments for protein-protein interaction... -
Deisseroth INTRSECT Collection
TypeCollection...using multiple-feature Boolean logic. Nat Methods 11(7):763-72. PubMed (Link opens in a new window) Chuhma... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...This method resulted in efficiencies ranging from 11-100% of F1 animals carrying precise knock-ins, and... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...Stains and fluorescent dyes. Nature Cell Biology, 11(S1), S7. https://doi.org/10.1038/ncb1939 Liu, X.... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post...PMID: 21527931. PubMed Central PMCID: PMC4772972. 11. Salomonnson, Emma, et al. "Cell-based and in vivo... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...PMID: 23667515. PubMed Central PMCID: PMC3647052. 11. Stryer, Lubert. "Fluorescence energy transfer as... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...pages using the links at the top of the page. Figure 11: A completed plasmid page for review before submission... -
CRISPR History and Development for Genome Engineering
TypeCollection...classification of CRISPR-Cas systems. Nat Rev Microbiol . 13(11):722-36. PMID: 26411297 Mali P, Yang L, Esvelt KM... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection...genetic access to cortical cell types. Cell , 188 (11), 3045–3064. https://doi.org/10.1016/j.cell.2025.05.002... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...analysis, and machine learning. Nature Biotechnology, 39(11), 1414–1425. https://doi.org/10.1038/s41587-021-00938... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post... an ever increasing marketplace. Since 2013 about 11 tools have been added every year. Breaking down... -
CRISPR/Cas9 FAQs Answered!
TypeBlog Post...using the CRISPR-Cas9 system. Nature Protocols, 8(11), 2281–2308. https://doi.org/10.1038/nprot.2013.143... -
Chemogenetics Guide
TypeGuide... to. Gq-DREADDs Gq-DREADDs signal through the Gαq/11 G-protein and activate neuronal firing through stimulating... by FRET-modified bioluminescence. Neurophotonics 11 (2), 021005. https://doi.org/10.1117/1.NPh.11.2.021005...with improved coupling efficiencies. Neurophotonics, 11 (2), 024208. https://doi.org/10.1117/1.NPh.11.2.024208... -
CRISPR Guide
TypeGuide...orthologs include Hsp1Cas9 (from Helicobacter sp. MIT 11-5569 ) and Nme2Cas9 (from Neisseria meningitidis ...orthogonal RNA/DNA labeling in the same cell. Figure 11: Overview of visualizing genomic loci with fluorophores...optogenetic control of CRISPR–Cas9. Nature Methods , 15 (11), 924–927. https://doi.org/10.1038/s41592-018-0178...CRISPR–Cas systems. Nature Reviews Microbiology , 13 (11), 722–736. https://doi.org/10.1038/nrmicro3569 PMID...using the CRISPR-Cas9 system. Nature Protocols , 8 (11), 2281–2308. https://doi.org/10.1038/nprot.2013.143...double-stranded breaks. Biochemical Journal . 475 (11), 1955–1964. https://doi.org/10.1042/BCJ20170793 ...genes and phage host factors. PLOS Genetics . 14 (11), e1007749. https://doi.org/10.1371/journal.pgen.1007749...