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GFP is the most widely used fluorescent protein, but there are several instances that limits its use. GFP requires oxygen, is sensitive to acid, and its large size can be problematic. When thinking about ways to label proteins, a different probe may be more appropriate.

Explore empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene of interest.

The Rinehart Lab has developed several tools useful for the incorporation of phosphoserine into your gene of interest.

Follow this protocol to produce adeno-associated virus (AAV) in HEK293 cells.

What is research? How do I find a lab? How should I approach research after finding a lab? Get answers to these questions and more about undergrad research.

Learn how FLEx and optogenetics combined can be combined to control gene expression with high spatial and temporal resolution.

Lambda Red mediated recombineering can be used to generate point mutations in plasmids and bacterial genomes. Learn how to use Lambda Red in this post.

Learn about the pros and cons of biased and unbiased CRISPR genotyping methods.

Broswe plasmids and other research tools for use in Rett Syndrome research.

There are many factors to consider when choosing a fluorescent dye or label. This post walks you through them so you can select a suitable one for your experiment.

Learn about the process to select the optimal serotype, promoter, and titer for your AAV experiment.

Recent developments using CRISPR/Cas9 editing with C. elegans, focusing on the use of an embryonic lethal selection marker to select for knock-in.

Learn about fluorescent protein techniques you can use to monitor cellular trafficking throughout an organism.

Learn how to target donor and acceptor fluorophores for FRET experiments.

Explore Addgene's collection of luciferase plasmids for gene expression assays and bioluminescent reporters.

Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral expression of molecular payloads in target populations defined by multiple parameters.

Need help with the plasmid deposit process at Addgene? Consult this post for an in-depth walk through the entire process.

This post describes CRISPR-derived base editors to convert cytosine to adenine or guanine, called CGBEs or GBEs.

It can be difficult to choose the best CRISPR software tool. The CRISPR Software Matchmaker makes choosing a guide RNA design software easier.

An overview of CRISPR's development from a prokaryotic immune system to a valuable genome engineering tool.