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We narrowed to 339 results for: ADA

Showing: 141 - 160 of 339 results

  1. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...routinely in biotechnology to transform plant cells. Degradative plasmids: microbial hardiness There are also ...organisms. To conserve energy, these specialized degradative capabilities are often tightly regulated to be...

  4. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ... the simplest CRISPR systems and the easiest to adapt to genome editing, but the introduction of type ...positions are indicated below each graph. Figure adapted from Kleinstiver et al., 2016. Until recently...

  5. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...longevity in eukaryotes and promote transcript degradation in prokaryotes.  Prokaryotic termination   Prokaryotic...important for stability of the mRNA, protection from degradation, and is integral to the nuclear export and translation...

  6. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...References and Resources References Braun, C. J., Adames, A. C., Saur, D., & Rad, R. (2022). Tutorial: design...Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal, T., Yadanar, L., Tian, R., Ma, K., Palop, J. J., & Kampmann...

  7. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...MagLOV’s parent protein AsLOV2 has already been adapted into a variety of optogenetic tools, they’re optimistic...optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing science...

  8. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...the kinetic response time from signal input to readable output, which generally exceeds that of the VSD...of the previously described systems. Several adaptations of this method have been to tag, cage, uncage...

  9. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...interference (CRISPRi) has been limited to lab-adapted bacteria because it has been challenging to introduce...for using CRISPRi in many types of bacteria: lab-adapted, environmental isolated, and disease-associated...

  10. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ... co-purify with your plasmid DNA and lead to degradation of your prep. Many plasmid prep kits recommend...strains are not good for cloning Bacterial strains adapted for protein purification use the cell’s resources...

  11. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...disadvantages of the IRES element, scientists have adapted "self-cleaving" 2A peptides into their muliticistronic...blog posts and additional resources into one downloadable PDF that you can save on your desktop. The Plasmids...

  12. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...resistance to both carb and amp, due to their common degradation mechanism. How to choose between the two? Carb...high pH environments and is less susceptible to degradation in agar plates. This prevents satellite colonies...

  13. An Introduction to Adenovirus

    Type
    Blog Post
    ...cellular events, all recruit strong innate and adaptive immune responses that inactivate viral genomes..., J. S., Lovett, S. P., Parker, K., Radune, D., Hatada, A., Pan, C. Y., Rizzo, K., Bertumen, J. B., Morales...

  14. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...Brans, M. A. D., Luijendijk, M. C., Garner, K. M., & Adan, R. A. H. (2010). Optimization of Adeno-Associated..., Malaker, S. A., Poweleit, N., Maynard, L. H., Adams, C. M., Leib, R. D., Cirolia, G., Thomas, D., Stamnes...

  15. DNA Purification Without a Kit

    Type
    Blog Post
    ...or TE. The Bishop Lab’s glass milk protocol is adapted from the 1979 Patterson Protocol. When published...100 bp to 48 kb with high yields and without degradation during the extraction procedure. Additionally...

  18. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...ubiquitination substrate Ikaros (IKZF1ZF2) (B). Image adapted from Kroupova et al., 2024 under CC BY license....design and studies of protein ubiquitination and degradation.   Find the CRBNmidi plasmid here!   Kroupova...

  19. Hot Plasmids - November 2023

    Type
    Blog Post
    ...trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023 bioRxiv (A) and ...:GFP-iPAK4 at 3 or 6 h. Scale bar 5 µm. Images adapted from Lin et al., 2023 bioRxiv, under CC-BY-NC 4.0...
Showing: 141 - 160 of 339 results