We narrowed to 200 results for: ADAP

1. Mouse Modeling, Part 1: Genetically Engineered Mice

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   ...mouse models provide researchers with useful and adaptable tools to perform basic and preclinical research...

2. Hot Plasmids February 2024

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   ...by co-incubation of Cas-RNP and assist peptide. Adapted from Zhang et al. 2023 with permission.   Find...

3. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   ...through error-free homologous recombination (HR). Adapted from an image provided by Manuel Gonçalves; made...

4. Plasmids 101: Protein Expression

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   ... grown in well-defined media and can be easily adapted to fermentation allowing for large-scale, stable...

5. Viral Vectors 101: Voltage Indicators

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   ...of the previously described systems. Several adaptations of this method have been to tag, cage, uncage...

6. Truncated gRNAs for Regulating Gene Expression

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   ...interacts with a ≤15nt gRNA that contains an RNA adapter capable of recruiting a transcriptional activator...

7. Plasmids 101: Knockout/Knock-In Plasmids

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   ...them by depositing with Addgene! Figures were adapted from: Hall B, A Limaye, & AB Kulkarni. (2009) "...

8. Twenty Years of Addgene Sharing: CRISPR

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   ...our requestors. However, we can again see quick adaptation and steady use of all types of libraries, once...

9. Plasmids 101: Degron Tags

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   ...to ubiquitination and proteasomal degradation. Adapted from Nabet et al., Chemical Biology, 2018.  ...

10. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    ...colocalization analysis example is given in Figure 2 (adapted from 4) - Dunn et al. 2011 provides an in-depth...

11. Plasmids 101: 5 factors to help you choose the right cloning method

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    ...Gateway cloning protocol is fairly flexible and adaptable to cloning different types of DNA, such as cDNA...

12. Plasmids 101: Optimizing Plasmid Yields

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    ...strains are not good for cloning Bacterial strains adapted for protein purification use the cell’s resources...

13. Plasmids 101: Multicistronic Vectors

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    ...disadvantages of the IRES element, scientists have adapted "self-cleaving" 2A peptides into their muliticistronic...

14. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    ...medicine. The parts we have right now are well adapted to work in prokaryotic bacteria, not in fungal ...

15. CRISPR 101: RNA Editing with Cas13

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    ..., 2016) as a RNA-targeting CRISPR enzyme, they adapted Cas13b for precise RNA editing (Cox et al., 2017...

16. DNA Purification Without a Kit

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    ...or TE. The Bishop Lab’s glass milk protocol is adapted from the 1979 Patterson Protocol. When published...

17. A Guide to Starting Your Own Journal Club

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    ...group and topic. For example, it may be useful to adapt the second version (multiple presenter, figure by...

18. SciComm: Taking Science to Elementary and Middle Schools

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    ...formulated the presentation as follows (which could be adapted for any topic--not just CRISPR): Who am I? Where...

19. An Introduction to Adenovirus

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    ...cellular events, all recruit strong innate and adaptive immune responses that inactivate viral genomes...

20. Getting to Know Expansion Microscopy

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    ...model systems, paired with SRM techniques, and adapted for detecting nucleic acids as well as proteins...