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We narrowed to 953 results for: APP

Showing: 141 - 160 of 953 results

  1. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...our guest bloggers Erik L. Snapp and Lindsey M. Costantini! Erik Lee Snapp received his PhD in Molecular... post was contributed by guest bloggers Erik L. Snapp and Lindsey M. Costantini. "You underestimate the... of a mystical "Force," this quote is equally applicable to many microscopy experiments with fluorescent...not fluoresce. A dark population is not readily apparent and can confound quantitative imaging experiments...for use in cytoplasmic environments. However, approximately 40% of human (and most eukaryotic) proteins ...have reported that FP cysteine residues form inappropriate disulfide bonds in the secretory pathway (Figure...localization. For example, the formation of inappropriate inter-chain disulfide bonds between FPs blocks...

  2. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...developed and applied. The current pandemic serves as a striking example of the applicability and importance...research. I applied, hoping that the experience would improve my scientific and application writing skills...science (as opposed to translational, biomedical, or applied sciences), people conduct research to answer questions...revelation. Science in any field can generally be applied to improve our lives and solve global problems....grants Varying degrees of engagement with what happens in the lab Official title is typically Professor...the people around you. Be proactive Rather than applying only to posted job offerings, the best research...ties into the previous one too. I would recommend applying for multiple positions—don’t put all your eggs...

  3. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...received 116,224 employment based green card applications and approved 91,023 during the first 3 quarters of ...options discussed in this post benefit from the applicant thinking about the process early, keeping good...is a numerical quota of 85,000 total H-1B visas approved each fiscal year: 65,000 visas plus 20,000 reserved...occupation" as requiring theoretical and practical application of a body of highly specialized knowledge in ... April 1 deadline for companies to submit an application on their behalf.  If an individual obtains a ... cap subject H-1B (last year less than 30% of applicants received sponsorship) then he or she can start...1B petitioners are exempt from the cap if the applicant works at institutions of higher education, a related...

  4. Evolution of Lab Techniques

    Type
    Blog Post
    ...real-time PCR (4). Restriction mapping and modern sequencing Restriction mapping informs on DNA sequence through...useful applications and have informed many current techniques. For example, restriction mapping led to...historical techniques: southern blotting, restriction mapping, and sequencing gels, as well as their modern equivalents...comparison to a control sample. Southern blotting applications range from confirming the results of cloning...gene that causes sickle cell anemia. The mutation happens to destroy a sequence recognized by the restriction... was commonplace, researchers used restriction mapping to investigate DNA sequence content by comparing...of the DNA. Starting in the 1970s, restriction mapping was commonly used to map DNA and examine genomes...

  5. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...zebrafish embryo has been mapped using single cell RNA sequencing approaches (Harland, 2018). Several ...investigations. Genetic resources for zebrafish A common approach to unravel the gene networks that drive biology...DNA into the genome is useful for a variety of applications. For example, specific tissues such as blood... regions is also possible using an alternative approach that exploits the bacteriophage integrase phiC31...useful, I’ll outline two recent lineage tracing approaches that led to important insights in developmental...tracked using microscopy and advanced computational approaches (Keller et al., 2008). This microscopy-based ...pathways control this process. Additional zebrafish applications and conclusions Zebrafish are also popular outside...

  6. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...subsequent, repeated activation), this is an approximation of reward or the sense of pleasure that the ...EthoVision. Consult the literature to find all the applications of optogenetics relevant to your field. Ultimately...behavior, certain technical considerations still apply. Testing stimulation First, In lieu of electrophysiology...stimulate them for 30min to an hour and observe what happens. You never know how stimulation of a particular...optimization, turn on the laser and watch what happens. Who knows, you may observe something that could... discovery. Monitoring behavioral output The appropriate behavioral readout for your optogenetics experiment...brain region that may be important in memory, an appropriate behavioral test might be contextual fear conditioning...

  7. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... a bioinformatics approach examining genomes possessing CRISPR systems with apparent self-targeting. Two... plasmid or group of plasmids' functions and applications. We hope that these articles make it easier ...includes four promoters, six fluorophores with nonoverlapping emission spectra (N- and C-terminal tags for...Targeting and manipulating interneurons using a viral approach Genetically modified organisms like transgenic...successfully targeted via viral particles, an effective approach to target specific neuronal subtypes was not available..., Gordon Fishell’s lab has developed a new AAV approach using regulatory elements that restrict expression...tools in virtually any vertebrate. This novel AAV approach allows you to target and functionally manipulate...

  8. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ... matches to your insert. Select the appropriate gene if it appears in the Entrez Gene list, as this will...would search for it in PubMed and choose the appropriate publication. This publication will be listed ...preprint. In the case of a preprint submission, we are happy to update the publication information once the manuscript...pass our standard QC process and the MTA has been approved. The spreadsheet option is recommended for ...contain epitope tags or fusion proteins that are appended to new inserts through cloning. *Note — If you...complete these pages sequentially by entering the appropriate data and pressing "Save and Continue to Next ...around to the different pages by clicking on the appropriate boxes at the top of the page. Pro Tip! Be sure...

  9. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...the filament from (a-b), showing the appearance and disappearance of 649-mDia1 or 549-CP (upper, middle...block its catalytic activity and instead become trapped in a covalent bond with its substrate. Then, all...having to clone a whole new construct for every application. Plus, the photophysical properties of chemical...advantages over fluorescent proteins, opening up new applications.   Figure 1: Live-cell labeling strategies...acids 1 Various Many Complicated   Applications Organic dyes are typically both brighter and...self-labeling tag and a fluorescent protein) enables applications like multi-color imaging (Figure 2). If you’...Actin, cyan), with formin (649-mDia1, magenta) and capping protein (549-CP, yellow) at the tip. 649-mDia1 ...

  10. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...depending on the conference you’re attending and who approaches you. You might also have cross-team projects ...communication. Although these real world examples did not happen in the lab, they have parallels to collaborative...types or purposes of each given container, when applicable Any other relevant information Prescribing an...misinterpretation, that misinterpretation was definitely happening. Our solution for this problem was the creation...lab setting, where student and postdoc turnover happens regularly and it behooves everyone in the lab to...potentially ambiguous terms. Defining words and applying annotation can be particularly useful in academic.... But if you implement it well, here’s what can happen: More effective meetings. When everyone at a meeting...

  11. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ... use one of two common approaches — columns or magnetic beads. Both approaches involve binding the plasmid... plasmid preps are the same. Your downstream application will dictate how much DNA you need to recover...recover and the level of quality required. Some applications require very little DNA and you can get away with...with slightly lower quality preps, while other applications require large amounts of high quality DNA. Figure... a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self explanatory and...possibility of damaging the plasmid DNA itself, which can happen if you leave the sample in a lysis buffer for too... the DNA from the substrate. In a column-based approach, the substrate is a silica matrix fixed in a column...

  12. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...truth is, there is a lot of software development happening at Addgene under the hood. A significant portion...this can lead to wildly different ideas of how to approach a problem, but it is these very differences that...similar cognitive biases and this would become apparent in the software we develop. We would all have ...framework is based on an iterative and collaborative approach to development. Scrum teams plan and break up ...are also able to consider different technical approaches that may come up during discussion.  One example...perspective which in turn helps us decide how to best approach it in our code.  Sometimes while thinking through...that the clarifications lead to the story being scrapped or broken up into smaller stories. Since this ...

  13. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...virus If having happy cells is the first step of a successful lentiviral infection, happy virus is the second... express a gene of interest. While the general approach for using lentivirus, infect and select, seems... cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels of contamination. For...Uphoff et. al. outline a do it yourself PCR-based approach complete with freely available positive controls...old. Ensuring that you are working with healthy, happy cells is one of the easiest ways to improve both...envelope. One drawback of the multiple harvest approach is that it tends to result in a higher volume ... freshly collected lentivirus for downstream applications. While this ensures that the highest titer virus...

  14. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ...non-refundable fee is applied to cover processing costs. Once the application is approved, Addgene then ships...excited to announce we now offer an export license application for rabies materials! Some of the materials in...international requestors. We also offer export license applications for VSV and SARS-COV-1 plasmids. Export control...license process    An export control license, once approved, allows Addgene to ship plasmids or viral vectors...will need to provide a brief description of the applicable research project when purchasing export-controlled...export-controlled materials. Addgene will then apply for the license for distribution to the requester’s organization...offered VSV and SARS-COV-1 plasmids under this application process since May 2020. We find this process ...

  15. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...led to their application for basic and translational research.  Basic research applications of scFvs: ...blood clearance, which is useful for imaging applications; better tissue penetration which is useful for... for therapeutic and imaging applications; and reduced immunogenicity when administered in vivo due to...from blood can be a drawback for therapeutic applications where longer retention times often increase ...laborious.  To avoid this, one or more of these approaches are often used for scFv expression in bacteria...since it’s genetically encoded.   Translational applications of scFvs: Just like antibodies, there are many...2019).Many of the scFvs designed for oncology applications are reformatted as bispecific molecules which...

  16. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...fits all When choosing an appropriate antibody first look at the application-specific data provided on...your specific application. Consider also that there may be variations within an application. Take immunohistochemistry... a linear antigen tend to work very well for applications that denature a protein to its primary (linear...while the website highlights a variation of your application, a publication successfully used it in a similar...plan to. To secondary or not to secondary?  The application will also determine whether you should use an... as it will help to amplify the signal. Some applications use either direct (primary-conjugated) or indirect... are secondary considerations to validation, application specificity, and experimental considerations ...

  17. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...many applications, it is the only comprehensive method available. Targeted sequencing approaches More...differently. For a comprehensive approach, RNA-seq would be an appropriate method for an activator or repressor...lower them even further. While not all of these approaches will work for every experiment, at least one ...where only localization of the Cas occurs. 2 gRNA approach Off-targets that come from mutations arise from...More targeted alternative sequencing approaches have been developed. Cas ChIP-Seq, where only DNA sites ...especially when DNA mismatches are involved. Two other approaches have been used - GUIDE-Seq and integrase-defective...site-specific detection downstream. The con to these approaches is that NHEJ must perform the repair to allow...

  18. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...your experiment (all well validated for your application, of course!) When the stars are aligned and the...factors that affect isotype choice. Target and application should be considered The first question you may... a strong signal and to work in a variety of applications. The desired specificity of your secondary antibody...affect my experiment?” Well, it depends on the application and target. If you are running a basic western...target is expressed at very low levels or your application requires a high degree of affinity, then species...considered when choosing an antibody. In some applications probing species-on-species, i.e. using a mouse...easily distinguishable from each other. In a direct approach, that is simple - but what about when you need...

  19. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ... to be placed immediately upon request approval. Once approved, the request will remain in your account... in this blog post.     When will the expansion happen?  You will be able to request Packaged on Request...request will be reviewed by Addgene for legal approvals and technical compatibility with our production...process will take 1–2 days, and we anticipate approving the vast majority of requests.  If the vector ... request process.  Ordering If your request is approved, you will receive an email notification that your...is ready to order. An “Add to Cart” button will appear with the request in your Addgene account.  Orders...viral preps will be available at launch.  What happens after I order?  Once an order is placed, the Addgene...

  20. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...distinction of neighboring cells in imaging applications and the mapping of neuronal projections to their associated...while minimizing confounding secondary effects? An approach described as “knocksideways” (a British idiom ...domains, such as FKBP and FRB. The cell does not appear to mind extraneous proteins bound to its outer ...protein from expression to degradation. Chemical approaches may require expensive peptides or small molecules...and Technology Austria, decided to take a novel approach to control for these effects, drawing on the rapidly...optogenetics. Using a rational protein engineering approach, they designed Opto-RTKs, which activate signaling... on exposure to low-intensity blue light. This approach relies on the incorporation of light-oxygen-voltage...
Showing: 141 - 160 of 953 results