...instances. If your poster material is nearing publication, consider uploading your work to a preprint server... this is being used, it’s best to turn to an established social media platform. During the conference ...something on social media. If someone is presenting unpublished data they may ask the audience to not share their...

...do this internally, but they are not available publicly. Furthermore, we did not find any tools which ... is generally advised, there is little detail published on how to do it best. Scientists are typically...the How To Guide. Conclusion CrispyCrunch is now publicly-available for high-throughput design and analysis...

... for making targeted modifications. A recent publication by Zhang et al. shows just that - using MMEJ ...PMID: 26678082. Find the plasmids from this publication at Addgene. 2. Dalvai, Mathieu, et al. (2015...633. PMID: 26456817. Find plasmids from this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei...

...techniques. More and more CRISPR tools are being published each month, making CRISPR a great choice for your...to consult the literature to see if anyone has published a protocol that would work for your system. Addgene... are a few plasmids at Addgene that have been published as CRISPR testing tools: Traffic Light Reporter...

...early I’m fortunate to live in a town with a good public school system that has made strong efforts in the...them had imagined for a scientist. I think that establishing this history helped demonstrate that a) I was...examples I used in my presentation had only become public in the previous two weeks, which demonstrated to...

...emerging work by collaboratively discussing recent publications in a specific field(s). Most anyone who has ...stay up to date and engaged with new advances in published science. They also serve as a way to learn about...blog posts centered around scientific papers and publishing Find tips for leading a great meeting ...

...This post was originally published on the Quilt Genomics Blog and is republished here with permission. Quilt...identified 23-mer gRNA. Off-target determination was established with Bowtie2 using the parameters first described...

...protein instead localizes to the cytoplasm? Or more troubling, what if a significant fraction of the fusion ...properly? We suggest starting by using the newly published oxidative optimized palette of FPs (moxFPs) [5... non-optimized parental proteins. Our recent publication illustrated the quantitative increase in fluorescent...

...priorities in most cases include IP protection, publications and funding. If any of these three pillars crashes...not enough. A 2013 study showed that out of 238 published scientific articles, only 46% could be reproduced...there will be no sharp changes in costs once they establish the system within their institution. In the study...

...data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist can learn....room, so it is a good idea to start thinking about public speaking early and often. Two of my jobs have indirectly... done so. How can you expect to deliver a great public presentation if you are doing it for the first ...

...synthetic biology, mainly because of the ease in assembling multiple fragments in one step with no scar sequences...CRISPR technology and is described in a recent publication by the Lockey lab (Wang, et al. 2015). In this...PMC4569707. 4. Kahl, L. J. & Endy, D. A survey of enabling technologies in synthetic biology. J. Biol. Eng...

...the right information When using a previously published system in your own work, you’ll need to know (...break your experiments!  First, closely read the publication, including the supplemental data. You’re most... both of which have extensive collections of publications and information on hardware, optogenetics tools...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function Genome Editing...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Plasmid Gene/Insert Promoter Selectable Marker Publication Prime Edit Cas9 H840A nickase fused to a reverse...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Epigenetics To make targeted epigenetic modifications... ID Plasmid Gene/Insert Selectable Marker PI Publication RNA Knockdown Type VI CRISPR systems, including...

...need to use FACS (like they did in the original publication). I thought I could see GFP expression with a...expression, but I didn’t. 2. In the original publication they see GFP expression in about 20% of their...an MOI of 0.3, as recommended by the original publication. Pro-Tip: 1. Use multiple gRNAs to target different...

...speed at which a confocal image can be acquired. Assembling an image pixel by pixel is slow; therefore, certain...can a a high enough density of red-photons be establish for this to happen.Using red light has a second... be afraid to seek out expertise from a well-established microscopist at your institution when heading...

...Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid...Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans...Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Zebrafish...Marker PI Publication Xenopus ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Parasites...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

... intermediate pluripotent state is not always obligatory in cell conversion processes [35]. Rather than...Complete removal to convert to iPSCs and re-establishment after Partial rearrangement from one cell type... pursue a career in science communication and publishing after completing her postdoctoral training from...

...than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome Editing...opposite strand) mutations. ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse...template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a ...specific locus. ID Plasmid Gene/Insert Promoter PI Publication Interfere Catalytically dead dCas9, or dCas9 ...specific locus. ID Plasmid Gene/Insert Promoter PI Publication RNA Targeting Type VI CRISPR systems, including...the entire population. ID Plasmid Promoter PI Publication Purify A catalytically inactive Cas9 (dCas9) ...

...The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin... abundant targets: from variable nonsense to publication quality data. Sci Rep 7, 2409 (2017). https:/..., J. The Ultimate qPCR Experiment: Producing Publication Quality, Reproducible Data the First Time, Trends...

...Originally published Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems...the above CRISPRi section, the Marraffini Lab published a robust tool for CRISPRi and CRISPRa using RNAP-Omega-dCas9...RJ, Grossman AD, Gross CA, Rosenberg OS (2019) Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi...