Skip to main content
Addgene
Showing: 141 - 160 of 298 results

  1. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...Pubmed search for “AAV” and “brain” over the past decade gives over 22,000 results - an enormous amount ...L., Muñoz, K. A., Hsu, R., Kalwani, L., Sierra-Mercado, D., Robinson, J. O., Outram, S., Koenig, B. A....

  2. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ... the nucleic acid. Proceedings of the National Academy of Sciences of the United States of America, 54...genetic recombination. Proceedings of the National Academy of Sciences, 87(4), 1556–1560. PubMed PMID: 2304918...

  4. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...Ligand-Regulated Site-Specific Recombination.” Proc. Natl. Acad. Sci. USA 92(June): 5940–44. PubMed PMID: 7597057...Ligand-Dependent Chimeric Cre Recombinase.” Proc Natl Acad Sci U S A 92(15): 6991–95. PubMed PMID: 7624356....

  5. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...plasmid proliferation." Proceedings of the National Academy of Sciences 80.15 (1983): 4784-4788. PubMed PMID...bacterial cell death." Proceedings of the National Academy of Sciences 93.12 (1996): 6059-6063. PubMed PMID...

  6. The Challenges of Cell Culture

    Type
    Blog Post
    ...problems surrounding cell culture over the last few decades some pharmacologists have tended to avoid cell ...comparable studies to be performed on a cell line even decades after it is initially isolated. Regrettably this...

  7. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...spectroscopy and microscopy." Proceedings of the National Academy of Sciences 114.23 (2017): 6005-6009. PubMed PMID...luminescence imaging." Proceedings of the National Academy of Sciences (2015): 201418468. PubMed PMID: 25831507...

  8. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...genome engineering tools that are available to academic researchers through Addgene. CRISPR components...coordinate gene silencing in mature plants. Proc Natl Acad Sci USA 116:7543–7548 . https://doi.org/10.1073/...

  9. Five Popular Model Organisms

    Type
    Blog Post
    ...mediated labeling." Proceedings of the National Academy of Sciences 106.35 (2009): 14896-14901. PubMed ...Caenorhabditis elegans." Proceedings of the National Academy of Sciences 113.8 (2016): E1074-E1081. PubMed PMID...

  10. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...variables (scFvs)? scFvs weren’t developed for another decade when in 1988 they were cloned by two separate labs... Escherichia coli. Proceedings of the National Academy of Sciences 85:5879–5883 . https://doi.org/10.1073...

  11. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...The focus of this article will be talks for non-academic settings. Many of these guidelines will apply ...presentation, but, for more detail on the job talk for an academic position, see this very good article at NatureJobs...

  13. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... by tetracycline-responsive promoters. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5547-51. doi: 10.1073...derived from the trp and lac promoters.” Proc Natl Acad Sci U S A. 80(1) (1983): 21-5. PubMed PMID: 6337371...

  14. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...antigen structure. Proceedings of the National Academy of Sciences 76:3116–3120 . https://doi.org/10.1073...some applications. Proceedings of the National Academy of Sciences 76:4350–4354 . https://doi.org/10.1073...

  15. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...the Dorr Mountain with the rest of the skyline of Acadia National Park. I was confused. It was supposed ...professor at the Institute of Zoology, Chinese Academy of Sciences, and he is interested in using CRISPR...

  16. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...transposase tools for genome engineering.,” Proc. Natl. Acad. Sci. U. S. A., vol. 110, no. 25, pp. E2279–87, ...tool for conditional cell ablation.,” Proc. Natl. Acad. Sci. U. S. A., vol. 105, no. 38, pp. 14492–6, Sep...

  18. 27 Hot Plasmids from 2016

    Type
    Blog Post
    .... PubMed PMID: 25895059 Barnea, et al. Proc Natl Acad Sci U S A. 2008. PubMed PMID: 18165312 CIDAR MoClo...PubMed PMID: 24136966 Nyerges, et al. Proc Natl Acad Sci USA. 2016. PubMed PMID: 26884157 Aronshtam...protein chains or clusters. Veggiani, et al. Proc Natl Acad Sci U S A. 2016. PubMed PMID: 26787909 EasyClone...

  19. Antibodies 101: Validation

    Type
    Blog Post
    ...recommendations for improving antibody use and reliability (Roncador, et al., 2015; Taussig, et al., 2018; Uhlen, et...Nature 518:27–9. https://doi.org/10.1038/518027a  Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL...

  20. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...,” but taking the first step into the world of academic research can be quite daunting. During my first... are also not limited to working at a bench in academia or industry, so feel free to explore! Addgene ...
Showing: 141 - 160 of 298 results