We narrowed to 214 results for: DAP

1. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   ...sequence TOM20 (pTriEX Tom20 mVenus BoPD). In dark adapted cells expressing these plasmids, tgRFP fluorescence...

2. Summer SciComm: Preprints

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   ...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...

3. Plasmids 101: The protein expression toolbox

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   ... Fig. 3 dTag mechanism of protein degradation. Adapted from Nabet et al. Check out Addgene's Lentiviral...

4. Plasmids 101: Cre-lox

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   ...natural viral life-cycle, and researchers have adapted the components for use in genome manipulation. ...

5. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   ...locomotion. Mice may need to be given some time to adapt to a testing apparatus. General stress levels of...

6. Open Resources and Plasmid Tools For Studying C. elegans

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   ...Degradation System (AID) The Dernberg lab in 2015 adapted the AID system discovered in plants to conditionally...

7. Mouse Modeling, Part 1: Genetically Engineered Mice

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   ...mouse models provide researchers with useful and adaptable tools to perform basic and preclinical research...

8. Adenoviral Vector Production and Troubleshooting

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   ...which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated gene delivery...

9. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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   ...protein-protein interactions. We’re excited to see how you adapt similar FLINC biosensors to work in your lab. Find...

10. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ...methylation, leaving the target CpG unmethylated. Adapted from Sapozhnikov and Szyf 2021.   Let me leave...

11. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ...indirect ELISA (more details below) can also be adapted to a competitive format. Figure 2: Overview...

12. Hot Plasmids February 2024

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    ...by co-incubation of Cas-RNP and assist peptide. Adapted from Zhang et al. 2023 with permission.   Find...

13. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...library. This PCR step also adds the necessary adapter sequences for Illumina sequencing as well as unique...

14. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    ...through error-free homologous recombination (HR). Adapted from an image provided by Manuel Gonçalves; made...

15. Plasmids 101: 5 factors to help you choose the right cloning method

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    ...Gateway cloning protocol is fairly flexible and adaptable to cloning different types of DNA, such as cDNA...

16. Plasmids 101: Protein Expression

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    ... grown in well-defined media and can be easily adapted to fermentation allowing for large-scale, stable...

17. Truncated gRNAs for Regulating Gene Expression

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    ...interacts with a ≤15nt gRNA that contains an RNA adapter capable of recruiting a transcriptional activator...

18. Plasmids 101: Knockout/Knock-In Plasmids

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    ...them by depositing with Addgene! Figures were adapted from: Hall B, A Limaye, & AB Kulkarni. (2009) "...

19. Plasmids 101: Degron Tags

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    ...to ubiquitination and proteasomal degradation. Adapted from Nabet et al., (2018).   HaloTag The HaloTag...

20. Viral Vectors 101: Voltage Indicators

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    ...of the previously described systems. Several adaptations of this method have been to tag, cage, uncage...