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We narrowed to 773 results for: Dos

Showing: 141 - 160 of 773 results

  1. Science Career Options

    Type
    Blog Post
    ...leaving academia, but don’t quite know what you do want to do or even what careers are available to scientists... Science in the News Don't stumble into a career, do your research Once you have identified possible careers... part of team? Will you be coordinating projects? Do you need computer programming skills? Other things...

  2. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...identify ourselves as part of a network of independent Do-it-Yourself (DIY) biology laboratories working towards...involvement and provide all our members the opportunity to do molecular biology, we’ve developed community projects...publish protocols so that anyone can engineer yeast to do similar things. The focus is to make yeast engineering... few yeast plasmids from Addgene and are eager to do our first yeast transformations! Dennis Oleksyuk,...

  3. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...proteins, respectively. A plasmid's copy number has to do with the balance between positive and negative regulation...showing the standard features of a plasmid. So, how do you choose? Addgene Senior Scientist Marcy Patrick...have only one plasmid type in your cells at a time? Do you want to make a lot of your plasmid? Is the gene...for optimal results always pick a single colony and do not subculture directly from glycerol stocks, agar...

  4. Plasmid Cloning by PCR

    Type
    Blog Post
    ...given sequence. You want to choose enzymes that: Do not cut within your insert Are in the desired location...(usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Bonus: It is helpful...GAATTCATGTGGCATATCTCGAAGTAC-3'. Many restriction enzymes do not cut DNA efficiently at the end of a linear piece...any 6 bases, but you should ensure that the bases do not result in the formation of a hairpin structure...

  5. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...engineering of its function." Once scientists can do that, they will not only understand how the brain... neural basis of violence or aggression, it might do a lot to explain more difficult aspects of human ...decision making, to cure a human patient. We can't do it all by ourselves. Getting these tools out into...cells in arbitrary patterns in the brain. Addgene: Do you have any special tips or tricks for someone looking...

  6. All Antibodies

    Type
    Collection
    ...Reactivity Recommended Applications PI Return to Top Do you have suggestions for other antibodies that should...

  7. Plasmid Collections

    Type
    Collection
    ...Rett Syndrome Plasmid Collection and Resource Center Do you have suggestions for other plasmids that should...

  8. Optogenetics Plasmid Collection

    Type
    Collection
    ...Promoter Eligible for Viral Packaging PI Return to top Do you have suggestions for other plasmids that should...

  10. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...PI Publication Last reviewed on: January 30, 2025 Do you have suggestions for other plasmids that should...

  14. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    .... In the most practical terms, what GreenGate can do is “save you half a year,” in comparison to more ...sensitive protein,” he says. “There is nothing you can do about it but be gentle. If it no longer works, just...blocks will be available, making it easier for us to do our work.” References 1. Athanasios Lampropoulos...

  15. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...will turn yellow. Do NOT change the media (add 2-3 mL of fresh media once a week), and do NOT harvest the...transgenes, transduce quiescent and dividing cells, and do not integrate into the host’s genome. The main challenge...test for the presence of RCA in the viral stock by doing a plaque formation assay on A549 cells . Alternatively...at -80C. Purification and concentration kits that do not use CsCl are commercially available. Titer calculation...

  16. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...such as HEK and CHO do not seem to require this, while nearly all endocrine cells do. While not all of ...different densities, then dispose of the plates that do not appear to be yielding well-spaced clones. For...replicates of each clone in case of contamination. We do not normally use antibiotics. Lastly, and perhaps...experience, cell clones picked using the agarose method do not differ grossly from those isolated using cloning...

  17. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ... easily separate out your target population. How do I know if I will need compensation? To know if you...on another.  What does spillover look like and how do I fix it? When plotting two channels against each... two channels. It is something your computer will do for you to better analyze your results; it does NOT...samples. With time and practice, you may be able to do your compensation setup on your experimental samples...compensation to your experimental samples In order to do compensation on your experimental samples, you will...

  18. Transferable Skills: Negotiation

    Type
    Blog Post
    ...that “negotiation” was something that I would mainly do over the price of a used car. Later, as I applied...the time over everything from which experiment to do next to how much data is necessary before submitting... someone can feed their cells this weekend - why? Do they have an important plans? What is most important...they different from mine? What equipment purchases do we prioritize in a lab with both electrophysiologists...help from another person/team at the company. How do I negotiate with the person/team for help? What can...

  19. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the...biostatistician, and that one golden-handed technician who can do a certain difficult assay with her eyes closed. How...career that I can’t help but feel a bit dubious when I do come across one. Also, remember, one important feature...80 hours a week in the lab, guess what? You are doing the work of more than one person. A selfish part...person’s name on your next paper, but you are not doing yourself or your science any favors by trying to...suffered for it. Nobody knew what anyone else was doing, and there were times when some members really should...of the team’s goals, then that team is destined to do poorly. To some extent, it doesn’t even matter whether...

  20. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...purification you can do is a miniprep, but if you need larger quantities of DNA, you’ll need to do a midiprep or...systems. Read on for an in-depth breakdown of how to do perform restriction digests.   Overview of...any pieces cut out or off of them. An easy way to do this is gel purification. In gel purification, you...empty plasmid, you will still have colonies when you do not add ligase. If the colonies are a result of recipient...significantly more colonies when you add ligase. If you do not see any colonies, you should conduct a positive...
Showing: 141 - 160 of 773 results