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We narrowed to 824 results for: EED

Showing: 141 - 160 of 824 results

  1. Science Career Options

    Type
    Blog Post
    ... outdoor skills are needed in a variety of roles, including: Livestock Breeder Scientific Illustrator...are needed for the role, as this will give you an idea of what additional training you might need to obtain...also need to think of other aspects of yourself that make you stand out. For every role you need to show...Illustrator Plant Breeder Zoological Worker Conservationist Documentary Director Parasitology Botanist Nature... team? Will you be coordinating projects? Do you need computer programming skills? Other things to consider...

  2. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...protein – you first need the DNA sequence that encodes it. That DNA sequence then needs to be incorporated...systems contain all the parts needed to produce proteins without the need to handle a living, replicating...essentially giving the cell all the instructions it needs to produce that green fluorescent protein through...having live cells in a classroom presents equipment needs, such as shakers and incubators, and biosafety issues...shelf-stable until water is added and protein production proceeds. FD-CF pellets are easy for students to use in...in the classroom because the students simply need to add water to express a variety of proteins. FD-CF...

  3. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    Blog Post
    ...read comes to this: we need others to review our experimental designs and we need to be transparent about...expect that every study should be reproducible. Indeed, researchers are pushing the limits of knowledge...estimates reaching in the billions of dollars (Freedman et al 2015). Finally, as Pamela Hines of AAAS ...best apply these technologies to your own research needs. Other companies like NEB and ATCC can provide you...). Once you’ve completed your experiment, you’ll need to analyze your results. As biological researchers...to do modified analyses on published data if you need to. Similarly, FigShare also makes it easier to ...through validation are easier to understand. The need for great stories has, however, made it difficult...

  4. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ... do is a miniprep, but if you need larger quantities of DNA, you’ll need to do a midiprep or a maxiprep...either a forward or reverse orientation so you’ll need some way to verify that the insert ended up in the...overhangs or no overhangs after digestion, you will need to use a phosphatase to prevent re-circularization... just throw the digestion mixtures together. You need to isolate your insert and backbone from the enzymes...overhangs cut off of it via their different migration speeds; after running the gel for some duration of time...it looks like your ligation has worked, you will need to pick individual bacterial colonies and check ...(the more background, the more colonies you will need to pick) and grow overnight cultures for DNA purification...

  5. Finding Your Perfect Job After University

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    Blog Post
    ...Working long distance, you need to be very self-motivated, because you need to work independently most... I applied for this summer position as I felt I needed more practical lab experience to later gain a full...if it is wasn’t a good fit for me, then I didn’t need to explain why I changed jobs. I highly recommend...I enquired about roles in this field, most jobs needed at least 3 months experience working in a developing... with a different climate and culture, and also needed to be resourceful and adapt. By the end of my three...really get to do a lot of lab work but instead really need to be comfortable with programming. Working for ...different aspects of the company and gave me the freedom to design new projects and grow in the role. I ...

  6. GCE4All: Making Genetic Code Expansion Accessible

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    Blog Post
    ...while the technical skills needed to perform GCE are essentially those needed to make site-directed mutagenesis...requirements of use. First, and most obviously, you need a non-canonical amino acid, which is usually added...the media for uptake by the cells. Second, you’ll need tRNAs and tRNA synthetases specific to the ncAA ...cells via plasmid. Third, a second plasmid will be needed to introduce the gene of interest containing the...including media, cell type, and growth temperature) may need to be individually optimized for each of the three...leading to system-wide effects. And on top of that you need to worry about getting high enough expression of...protocol, to optimize and share, they’re soliciting feedback from scientists on which protocols would be broadly...

  7. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    Blog Post
    ... only a subset of base substitutions.  The need for speed: base editing before Cas lets go Figure...domain lets go of the DNA. ABEs are somewhat slow and need a strong Cas9 domain to “hold on” long enough to...of gene III to base editor activity and editing speed and consists of the following components: A plasmid...variant, the phage is not infectious. The editing speed dictates which phage variants can propagate faster...of gene III to base editor activity and editing speed. Created with BioRender.com. Introducing ABE8e...higher than ABE7.10. However, the increased editing speed and targeting scope of ABE8e comes with a price:...naturally occurring enzymes and functional domains speed up the development towards faster and more precise...

  8. Antibodies 101: ChIP

    Type
    Blog Post
    ...  If you’re working with a tissue sample, you’ll need to homogenize your sample before moving on to the...homogenizer, but bone or especially fibrous tissues may need extra steps.  Fragmenting  The next step is break... chromatin.  Whichever method you choose, you’ll need to reserve a small aliquot, split into two parts...antibody-bound samples. With a bead method, you’ll need to ensure you have a bead-only control alongside...Capturing), if you ask me.  Each of the three steps need to be optimized for each specific sample type. And...optimization of another. For instance, a sample may need more or less cross-linking to firmly attach the ...output assay. A longer sonication time could mean needing to increase crosslinking to counteract the effects...

  9. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    Blog Post
    ...If you’re cloning a plasmid, you’ll need a way to find the needle in the haystack: the one perfect clone...find your desired plasmid. In many cases, you will need a screening strategy as well. In a screen, you aren...selection. Instead, all cells survive and you will need to sort through them to find the desired clone. ...easily identify successful clones so you have to weed through fewer colonies after your experiments. As... DNA using lysed bacterial colonies, without the need to prep your plasmid. In this case, you’d be using... expect it to be. To get started, you will first need a primer that complements your plasmid sequence....primers can't provide all of the information you need, so you'll want to design a custom primer. Because...

  10. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...functioning lab, but it is for a scaleable lab. When you need to track the location, quality, growth, and legal... In order to get a plasmid to your lab bench, we need to (this is a little simplified): Find the appropriate...require starting over entirely, and our software needs to handle these cases. The following code examples...we’re sure our techs could figure it out if they needed to, they’d have to individually keep track of any...whole procedure. Other steps fail “soft” they simply need to be repeated. We also now have a good interface...easier, the time and capital cost of getting up to speed with a setup is almost always worth it. Because ...and tube model, we can interchange them easily as needed. In this case, the physical considerations of the...

  11. Lab Automation at Addgene

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    Blog Post
    ...single channel pipettes. These can speed up any project in which you need to do a lot of aliquoting. Simply...throughput DNA preps and other molecular work if we need it to. This gives us the oppotunity to use the FXP...to in live bacteria. For these shipments, we’ve needed to find a way to purify DNA from many bacterial...Universal Platform and it suits our plasmid prepping needs perfectly. Working with Qiagen, we developed a protocol...and can be easily programmed for a variety of lab needs. Addgene uses it to fill as many vials as it can...amount of agar. There is a tube that goes from the needle to the flask with agar in it. The agar sits on ...program the pipette with how much total sample you need and the volume of the smaller aliquots you’d like...

  12. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...an option to all researchers. Accuracy needed Do you need to call exact copy numbers or just general...partitioned into thousands of reactions, it does not need to be run in triplicates. ddPCR is an extremely ...experiment. You’ll have to consider:  Resources needed Most molecular biology labs and departments will...general trends? How confident do you need to be in your determination to move forward with your experiment... that provides you confidence in the results you need.  Whichever method you choose, resist the temptation...Muhamad, 2011). Ambiguous Taqman or ddPCR results need to be rerun in order to be called accurately, and...likely to find an application that matches your needs. Happy quantifying!  References and Resources More...

  13. CRISPR 101: Multiplex Expression of gRNAs

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    Blog Post
    ...with the simplest multiplexing situation: you only need to express two gRNAs at the same time. One system...scale up the number of gRNAs in your plasmid, you’ll need to use some assembly methods such as Golden Gate...step is necessary to generate all of the overhangs needed for the final ligation step. Plasmids are then ...pX330A-1x6. This customization means you don’t ever need to clone in filler sequences. To build your multiplexing...with the kit. Oligonucleotides specifying the gRNA seed sequence should be compatible with type IIS enzyme...above, this vector does not contain Cas9, so you’ll need to supply it with another plasmid.   Figure...fast protocol, with only 5 hours incubation time needed for a single round of editing, and a subsequent...

  14. How to Be an Excellent Trainee

    Type
    Blog Post
    ... mind helping me with….” vs. “I need help with…now”. Ask for feedback so you can make adjustments. This...information plus your mentor will get you up to speed easier and faster when you’re an active participant...t be afraid to ask your bench mentor if/when you need to double-check a detail! Training follow up After...then plan accordingly in case you have questions or need a little assistance at some point during the procedure... day. In the latter case, you can say “I usually need time to gather my thoughts, but always have questions...learns where you are at and the level of support you need from them, and you can understand the information...are polite, appreciative, and clear about their needs. Remember, your bench mentor’s training time is ...

  15. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ..., we need to research and hopefully find a comparable library if it’s gone stale or we will need to update...flexibility to adapt and easily respond to changing needs of the teams at Addgene. Another advantage of Scrum...while thinking through these steps, we discover we need additional information. It is quite possible that...time efficiently. While writing the code, we may need additional clarification and again we collaborate... buying a bag of flour is not the only thing you need to make a cake. The flavor, shape, and texture of...today that prints labels. These libraries in turn need to stay up to date with their third party libraries... to Modern Greek. Books written in Ancient Greek need to be translated into Modern Greek to be easily ...

  16. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...based on its market need and anticipated commercial success. Or the client might need help with the intricate...client may offer a lot of pointed feedback or abruptly change needs and expectations. Such situations ...doing it. I’ll also talk about the skills you’ll need to do well as a healthcare consultant and what you...Medical Affairs, and Commercial teams, reflecting the need for all parts of a company to collaborate whenever...especially challenging. For instance, the client might need to get a large amount of market research done to... fulfilling career solving hard problems or who needs a stepping-stone to many other opportunities. Many...

  17. IDP and your PI: A Roadmap for Career Planning and Personal Development

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    Blog Post
    ...these meetings is to give honest, constructive feedback, set tangible research and career goals, and celebrate...development through thoughtful planning and honest feedback DePace’s yearly planning meetings revolve around...accomplishments, research/professional goals and feedback. Calendar: Month-by-month look at the next... year, including major project goals and effort needed to complete them. These documents dictate the subject...trainee leads the conversation. As the conversation proceeds through the various sections, the trainee speaks...Supervisors Management for Scientists: Seeking Feedback Management for Scientists: What Makes a Good Manager... feedback...

  18. CRISPR 101: Validating Your Genome Edit

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    Blog Post
    ...target cells and the desired edits. First, you will need to know what the ploidy of your target site is. ...line, so it’s important to verify that you won’t need to screen hundreds of clones looking for your edit...This way you can ensure you aren’t looking for a needle in a haystack before going on to generate and screen...then a single, bulk validation may be enough to proceed with your experimental plan. Validating knockout...generate your knockout? Then you’re in luck! You don’t need to sequence your PCR to visualize an edit. Most ...clones, however, sequence confirmation is still needed to verify the correct sequence was inserted or ... edited cells. As with all other methods, you’ll need to further validate the specific mutations using...

  19. Transferable Skills Guide: Time Management

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    Blog Post
    ... and by offering advice on areas where you might need some help. Today in our transferable skills guide...something like, “I’ll be there in a few minutes, I just need to split some cells.” Inevitably, this task would...techniques of your own, but if you ever find yourself in need of a few ideas, I hope you’ll find this post useful...the energy to finish any additional work you might need to do. In most cases, I would recommend the second...outset of a task, I outline the activities that are needed to accomplish these goals. Practically speaking... remind myself of the specific activities that I need to work on. Throughout the day, I cross items off...they are accomplished or I add to the list as the need arises.Why do I write things down? It’s more viscerally...

  20. Antibodies 101: Epitope Availability

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    Blog Post
    ...may only need to validate on denatured proteins or on live cells. But for others, you may need to validate...well-organized, documented, and easy to find (though we might need to see it to believe it.) As you browse the collection... affect its configuration. You don't necessarily need to know where the epitopes are, just if the available...selected an antibody, or multiple antibodies, you’ll need to validate them for your protein and its configuration...configuration in your assay. For some assays, you may only need to validate for recognizing the protein following...designing your controls in validation, as you may need to test different configurations and conditions ...
Showing: 141 - 160 of 824 results