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We narrowed to 224 results for: ENDO 2

Showing: 141 - 160 of 224 results

  1. Antibodies 101: Validation

    Type
    Blog Post
    ...another. You’ll need to validate that points 1 and 2 hold true under your specific experimental conditions...when (1) other strategies may not be feasible and (2) large-scale proteomic and transcriptomic datasets...that do not express your protein of interest endogenously and transfect them with a plasmid encoding your...ultimately planning to use your antibody to detect endogenous levels of the target in other cells or tissues...

  2. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...types within a sample to expected labeling (Figure 2). The panel of antibodies used in this approach should...the antibody in the same tube.     Figure 2: Schematic showing the use of an orthogonal approach...detect the antigen of interest when expressed at endogenous levels (Laflamme et al., 2019). CRISPR-Cas9 approaches... since there can be large differences between endogenous expression and transfected overexpression, this...cannot confirm the antibody’s ability to detect endogenous levels of the target protein. It may only be ...overexpression. Additionally, if the cell line used has endogenous levels of expression, this can make the results...different antibody clones produced by different vendors. The specific epitope recognized by either antibody...

  3. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...linear region to solve for total expression time. 2. Timer functionality with a single gene Although it...290.5496 (2000): 1585-1588. PubMed PMID: 11090358. 2. Tsuboi, Takashi, et al. "Age-dependent preferential...preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent...

  4. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...of these neurons via the channel Channelrhodopsin 2 (ChR2) reduced glutamate transmission from the mPFC...deliver Cre-dependent expression of  Channelrhodopsin 2 (ChR2), a modified receptor protein that activates...antidepressants-like effects in rats." Psychoneuroendocrinology 46 (2014): 78-87. PubMed PMID: 24882160...

  5. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ... to: (1) prevent vendor lock in by making it easy to get data out of the ELN; and (2) facilitate inter-operability...connectivity to other data sources used in research; (2) connectivity to other tools used in research; and...

  6. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...PMID: 20543841. PubMed Central PMCID: PMC2920597. 2. Nathans, Daniel. "Puromycin inhibition of protein...assay, primary antibodies directed towards two endogenous proteins of interests, suspected to interact,...take place if the two antibodies recognized an endogenous protein of interest that also had incorporated...possible to visualize specific newly synthesized endogenous proteins in situ. Now FUNCAT-PLA provides both...spatio-temporal information about the turnover of specific endogenous proteins in any cell type. Exciting! Many thanks...Ola, et al. "Direct observation of individual endogenous protein complexes in situ by proximity ligation...

  7. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...tagging." Nature methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and...Plasmids 101 Series. Get the Inntag Plasmids Tag endogenous proteins with CRISPR Fluorescently Tag Your Protein...

  8. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...dissociation by bioluminescence resonance energy transfer 2, or BRET2. With binding of the drug, the alpha and...coelenterazine, followed by the addition of the endogenous ligand or drug. In the inactive state, the luminescent...different GPCRs. This collection can be used to study endogenous ligands, the pathways they activate, as well ...

  9. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ... the transcriptional level.     Fig. 2 Tetracycline on/off promoters and modes of action ...expression, you are almost certainly working with an endogenous locus. The most straightforward way to turn off...When transiently introduced, they can deplete endogenous protein levels for 1-3 days. There’s also a ...degradation. These systems work by tagging the endogenously or exogenously expressed protein with the relevant...However, if you would like tunable control of an endogenously expressed protein, then these systems need to...

  10. All in a Twist: dsRNA

    Type
    Blog Post
    ... release them into the cytosol. Fig. 2: Endogenous sources of duplexed RNA DNA:RNA hybrids ..., as is often the case upon viral infection. Endogenously produced dsRNA rarely reach this threshold and...sensors are mutated or mechanisms which suppress endogenous dsRNA formation are dysregulated, immune responses...mount in the absence of infection. Similarly, if endogenously synthesized dsRNA finds its way outside of the...

  12. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...closely mimicking the future application. In figure 2 we provide an example of the comparison of the practical... is to tag an endogenous gene. By imaging cells (or tissues) that produce an endogenous protein tagged...employing co-expressing fluorescent proteins or using endogenous tagged genes) can be used to verify performance...

  13. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ... ligands can elicit varying degrees of β-arrestin-2 recruitment to receptors, indicating their agonistic...fluorescent labels. In Nature Methods (Vol. 19, Issue 2, pp. 149–158). Nature Research. https://doi.org/10.1038...a molecular species with high affinity for an endogenous molecular target, meaning the dye will be attached...results in direct binding between the dye and the endogenous target, and thus no genetically encoded tag is...

  14. Hot Plasmids February 2024

    Type
    Blog Post
    ...overview of these new variants:   Figure 2: Inner mitochondrial membranes imaged with structured...for systemic gene transfer in primate brains Endothelial-specific AAV delivery Recombinant anti-FLAG tag... it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA...10.1038/s41565-023-01419-x. PMID: 37430038. Endothelial-specific AAV delivery By Cythina Arokiaraj (Gradinaru...to that of AAV1. This viral vector has brain endothelial cell-specific tropism, with slightly reduced ...proof-of-concept study, mouse LY6A was delivered to brain endothelial cells in mice lacking LY6A using AAV1-X1 (Chen...X1 and AAV9-X1.1 efficiently transduce brain endothelial cells across diverse mice strains and rats. Figure...

  15. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...account when simulating a digest as depicted in Lane 2. Controlling methylation Finally, you can control...prevent them from being degraded by restriction endonucleases. A given prokaryote typically has genes encoding...groups to specific DNA sequences and companion endonucleases that recognizes and cleave the same DNA sequence...

  16. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...phosphoinositides (Varnai and Balla, 2008) (Figure 2). RNA production can be detected by using a MS2 coat... 296.5569 (2002): 913-916. PubMed PMID: 11988576. 2. Tsien, Roger Y. "The green fluorescent protein." ...requiring anaerobic conditions, proteins that bind endogenous fluorescent co-factors can be used. Examples ...

  17. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...wheat germ, HeLa, and Leishmania (Gagoski, et al 2015[2]). The proteins encoded in this set range in size ...to generate Illumina sequencing libraries using a 2-step PCR-based protocol and are compatible with single-end...Choudhury, Rajarshi, et al. "Engineering RNA endonucleases with customized sequence specificities." Nature...

  19. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...double strand break), increasing editing efficiencies 2-3-fold. However, the process of double nicking increases... editing as efficient as possible. Figure 2: Decision tree for selecting a PE6 variant. PE = prime...using paired prime editing. Nature Biotechnology, 40(2), 218–226. https://doi.org/10.1038/s41587-021-01025...Adamson, B. (2024). Improving prime editing with an endogenous small RNA-binding protein. Nature, 628(8008),...

  20. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...available in the adenovirus section below!   Fig. 2 - Viral vector components of AAV Adenovirus Adenoviruses...virion assembly and particle release from late endosomes when AAV is trafficked. Rep and cap mediate AAV...Of note, recombinant adenovirus is missing the endogenous E1 gene, making it replication deficient. This...
Showing: 141 - 160 of 224 results