Skip to main content
Addgene
Showing: 141 - 160 of 365 results

  2. What's New in CRISPR - December 2019

    Type
    Blog Post
    ... and A-to-G base editors into one protein. They fused an activation-induced cytidine deaminase (AID) and...adenosine-to-inosine (A-to-I) RNA editor called REPAIR by fusing catalytically inactivated RNA-targeting Cas13 (...

  3. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ... e67902. Many imaging studies rely on direct fusion of fluorescent proteins (FPs) to a yeast gene of...epitope tag for creation of your own unique protein fusions. This collection is ideal for tandem affinity purification...

  5. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...The first functional SB system was synthesized by fusing and modifying various extinct transposases. Improvements...is made up of a fragment of the RPL13a promoter fused to a region of the RPL41 gene, to drive the selection...

  6. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ... common protein-only transduction strategy is to fuse a cell penetrating peptide (CPP) to a protein of...discovered in the HIV protein Tat. Although CPP fusions can translocate a cell membrane, the presence of...

  7. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...in human cells (Ma et al., 2015). To do so, they fused a different fluorescent protein to various Cas9 ...Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized to specific loci based on the guide RNA...

  9. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...useful for studies in non-human primates, where fusion fluorescent proteins can affect DREADD trafficking... (PV2527) 105679  AAV5 ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid...

  10. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...lab first thought to enable MBE by using Cas12a fused base editors (dCas12a-BE). However, the poor editing...efficiencies they found led them back towards Cas9 fused base editors (nCas9-BE), as Cas9 is mechanistically...

  11. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...with other pathways. Since these effectors are diffusible, they’re also not suitable for spatially limited...light-sensitive two-component system (TCS). Levskaya et al. fused a photosensory domain from cyanobacteria to a common...

  12. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...not appropriate for mammalian selection – do not confuse this with G418 (aka Geneticin). Neomycin should...mammalian expression – instead use G418. This can be confusing since the neo/kan gene confers G418 resistance...

  13. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...unit. Each particle has a viral envelope, which fuses with the target cell’s membrane, allowing the viral...determine what type of cell the virus will target and fuse with. In nature, this determines which organisms...

  14. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ... Screens and selections The common goals The confusion in differentiating between a screen and a selection...inappropriately categorized as screens! Why the confusion? The commonality between these methods is that...

  15. Hot Plasmids - May 2022

    Type
    Blog Post
    ...2021.    BLITZ uses the biotin ligase TurboID fused to a conditionally stabilized GFP-nanobody rather...rather than a specific protein of interest. This fusion protein can then be co-expressed in essentially any...

  16. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...cryopreserved hybridomas, immortalized cells created by fusing an activated B cell that produces the antibody ...light (VL) and variable heavy (VH) chain sequences. Fusion PCR was performed to create an amplicon that  was...

  18. To Each HIS Own

    Type
    Blog Post
    ...   Prior to the polyhistidine tag, alternative fusion tags were used, such as S. aureus’ Protein A (Moks... to scientists. If their protein of interest is fused to polyhistidine, then they can use an anti-HIS ...
Showing: 141 - 160 of 365 results