Addgene

We narrowed to 243 results for: GRN;

Showing: 141 - 160 of 243 results

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3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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Blog Post

...about High Fidelity Cas9 Variants How to Design Your gRNA Delivering CRISPR Ribonucleoproteins Resources ...Check Out Our CRISPR Guide Pages Find Validated gRNAs ...
CRISPR 101: Cytosine and Adenine Base Editors

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Blog Post

...nickase) fused to a deaminase that makes the edit A gRNA targeting Cas to a specific locus A target base ...CRISPR plasmids for base editing Find validated gRNAs ...
The 12 Days of CRISPR: 2021

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Blog Post

... day of CRISPR, Addgene gave to me: engineered pegRNAs to improve prime editing efficiency. On the third...
Twelve Amazing Guest Blog Contributors!

Type

Blog Post

...software that predicts efficiencies of prime editing gRNAs. PRIDICT Harvinder Virk and Michael Biddle, ...
Performing In Vivo CRISPR Screens Using the FITS Approach

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Blog Post

...types. A key feature of this system is a lentiviral gRNA expression vector (pRDA_526) that (1) enables efficient...
Addgene’s Top 10 Blog Posts from 2014

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Blog Post

...Most Popular Posts of 2014 How to Design Your gRNA for CRISPR Genome Editing By John Doench (Guest...
Editor's Choice, August 2016

Type

Blog Post

...the transitions at target sequences specified by gRNAs. Caveats, potential specificity issues, and other...
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

Type

Blog Post

..., programmable DNA cutting enzyme. Second, since gRNA and crRNA could be combined into one RNA to direct...
Viral Vectors 101: Parts of the AAV Transfer Plasmid

Type

Blog Post

...smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of interest. Promoter The promoter...
Three Tips for Preventing Viral Plasmid Recombination in Your Samples

Type

Blog Post

... Z, Ma X, Wang N, Huo Y-X (2020) Reversed paired-gRNA plasmid cloning strategy for efficient genome editing...
Viral Vectors 101: What is a Virus (and a Viral Vector)?

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Blog Post

...the case of retroviruses, reverse transcribe their gRNA into DNA, which is then transcribed into mRNA before...
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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Blog Post

... protein is 1,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is thermostable ...
Using Addgene's New Viral Service for Your Research

Type

Blog Post

...nickase, Cas9 activators, Cas9 repressors, select gRNAs, and CRISPR pooled libraries. Whether you’d like...
Even more elegant: Single injection CRISPR/Cas9 in C. elegans

Type

Blog Post

...3xFLAG-tag fusion proteins. The repair template and Cas9-sgRNA plasmid is then injected into the germline of young...
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Type

Blog Post

...call the LADL Anchor. Using specifically designed gRNAs, they can then recruit these LADL Anchors to genomic...
Using CRISPR/Cas9 to Edit Disease Out of the Genome

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Blog Post

...impact of off-target mutations, scientists are using sgRNA for selection and optimization. In addition, the...
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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Blog Post

...Through molecular biology teamwork. The guide RNA (gRNA) specifies the editing target site on the DNA, the...
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

Type

Blog Post

...with CasRx and either an unprocessed or processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating...
Improved Plasmid Maps Powered by SnapGene

Type

Blog Post

...commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display, you...
Viral Vectors 101: Gamma-Retroviral Packaging Systems

Type

Blog Post

...γ-Retroviral transfer plasmid encoding a transgene, sgRNA, or shRNA of interest: The transgene sequence is...

Showing: 141 - 160 of 243 results

Results per page:

3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

CRISPR 101: Cytosine and Adenine Base Editors

The 12 Days of CRISPR: 2021

Twelve Amazing Guest Blog Contributors!

Performing In Vivo CRISPR Screens Using the FITS Approach

Addgene’s Top 10 Blog Posts from 2014

Editor's Choice, August 2016

Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

Viral Vectors 101: Parts of the AAV Transfer Plasmid

Three Tips for Preventing Viral Plasmid Recombination in Your Samples

Viral Vectors 101: What is a Virus (and a Viral Vector)?

Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

Using Addgene's New Viral Service for Your Research

Even more elegant: Single injection CRISPR/Cas9 in C. elegans

Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Using CRISPR/Cas9 to Edit Disease Out of the Genome

Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

Improved Plasmid Maps Powered by SnapGene

Viral Vectors 101: Gamma-Retroviral Packaging Systems

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