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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 319 results for: INO

Showing: 141 - 160 of 319 results

  1. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...rule of thumb, we avoid target sites that code for amino acids near the N’ terminus of the protein, in order.... Likewise, we avoid target sites that code for amino acids close to the C’ terminus of the protein, to...

  3. Promoters

    Type
    Guide
    ...from Lac operon araBad Inducible by arabinose Promoter of the arabinose metabolic operon trp Repressible ...adaptor molecules that are responsible for bringing amino acids to the ribosome when proteins are being synthesized...

  5. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...incubate O/N at 37 °C. Pick 2 - 3 colonies and inoculate into a mini-prep culture. Perform mini-prep for...utilized. Choose a sequence-verified colony and inoculate into a maxi-prep culture. Prep size may be scaled...

  7. DNA Quantification

    Type
    Protocol
    ...Quantification DNA Quantification You may also like... Inoculating a Liquid Bacterial Culture Recovering Plasmid...

  8. Protocol - How to Design Primers

    Type
    Protocol
    ... also use a nucleotide-based molecular insert (inosine) instead of a regular nucleotide for broader pairing...

  11. Deep Dive: qPCR

    Type
    Blog Post
    ...corrections are a better choice (Tichopa, 2003. Marino, 2003) - an important note, as the other most commonly...pitfalls and potential. BioTechniques 26:112–115.Marino JH; Cook P; and Miller KS. 2003. Accurate and statistically...

  13. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...extracellular space. In addition, rAAV2-retro’s altered amino acid sequence might facilitate interaction with ...PubMed PMID: 1381034. Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaj, M.I., Isa, K., Mizukami, H., Ozawa...

  14. Immunocytochemistry

    Type
    Protocol
    ...24-well plate 4% Paraformaldehyde 5 mg/mL 4′,6-diamidino-2-phenylindole (DAPI) Bovine serum albumin (BSA...

  17. Pouring LB Agar Plates

    Type
    Protocol
    ... mg/mL 10 µg/mL Kanamycin 50 mg/mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg...

  18. Optogenetics Guide

    Type
    Guide
    ...explanations of microbial opsin and optical switch terminology....expressed under the control of the vesicular gamma aminobutyric acid (GABA) transporter (VGAT) and ChR2 is thus...

  19. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...Hordeaux, J., Yuan, Y., Clark, P. M., Wang, Q., Martino, R. A., Sims, J. J., Bell, P., Raymond, A., Stanford....017 Matsuzaki, Y., Konno, A., Mochizuki, R., Shinohara, Y., Nitta, K., Okada, Y., & Hirai, H. (2018)....
Showing: 141 - 160 of 319 results