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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 326 results for: INO

Showing: 141 - 160 of 326 results

  3. Zebrafish Plasmid Collection

    Type
    Collection
    ...transposons, Cre-mediated site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9...Gateway destination vectors (pGCS), bearing either amino- or carboxyl-terminal tags, including Myc, HA, Flag...

  8. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...serine phosphorylation by adding this phosphorylated amino acid to the genetic code of E. coli (Park et al....consist of a central phosphoserine site flanked by 15 amino acids on either side occurring within the parent...

  9. TALEN Plasmids and Kits

    Type
    Collection
    ...determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors. View Addgene'...DNA-binding ability of TALEs), 63 wild-type TAL amino acids after the repeat domain, and C-terminal SV40...

  12. Deep Dive: qPCR

    Type
    Blog Post
    ...corrections are a better choice (Tichopa, 2003. Marino, 2003) - an important note, as the other most commonly...pitfalls and potential. BioTechniques 26:112–115.Marino JH; Cook P; and Miller KS. 2003. Accurate and statistically...

  13. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...Gootenberg, J. S., Konermann, S., Trevino, A. E., Scott, D. A., Inoue, A., Matoba, S., Zhang, Y., & Zhang...

  14. Immunocytochemistry

    Type
    Protocol
    ...24-well plate 4% Paraformaldehyde 5 mg/mL 4′,6-diamidino-2-phenylindole (DAPI) Bovine serum albumin (BSA...

  15. Luciferase Plasmid Collection

    Type
    Collection
    ...into Large (LgBiT; 18 kDa) and Small (SmBiT; 11 amino acids) fragments for protein-protein interaction...fluorophore to Troponin C Ca 2+ binding domain. Luminopsins : Luciferase-opsin fusion proteins for bioluminescent...

  16. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...incubate O/N at 37 °C. Pick 2 - 3 colonies and inoculate into a mini-prep culture. Perform mini-prep for...utilized. Choose a sequence-verified colony and inoculate into a maxi-prep culture. Prep size may be scaled...

  18. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...extracellular space. In addition, rAAV2-retro’s altered amino acid sequence might facilitate interaction with ...PubMed PMID: 1381034. Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaj, M.I., Isa, K., Mizukami, H., Ozawa...

  20. Pouring LB Agar Plates

    Type
    Protocol
    ... mg/mL 10 µg/mL Kanamycin 50 mg/mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg...
Showing: 141 - 160 of 326 results