...Carcinoid Tumors Bradley Bernstein Broad Institute Identifying Altered Epigenetic States and Drivers in Intestinal...

...mAb Plasmid Collection CRISPR CRISPR-on: an RNA-guided transcriptional activator system - Jaenisch Locus-specific...

...also like... Human Kinase ORFs Genome Engineering Guide Empty-Backbone Resource Mitogen-activated protein...

...transduce many mammalian cell types. AAV is used in a wide range of experiments, both in vitro and in vivo....

...together based on type or application. Browse our guides to find the plasmids and science information you...

... community. To deposit, view our SynBio Deposit Guide or start our online deposit process . Browse SynBio...

... contain the Addgene ID # and the information necessary to indicate that this ID is managed by Addgene...within the Addgene catalog. These IDs allow you to unmistakably identify a plasmid available through Addgene...VQR-Blast, which has the Addgene ID #87155. The resolvable compact identifier format for lenti-Cas9-VQR is...will be assigned an Addgene ID #, RRID, and resolvable compact identifier. Addgene works hard to ensure...including plasmids. All plasmids at Addgene have unique ID numbers associated with them, which serve as unique...about each plasmid item. Given a specific Addgene ID # listed in a publication, you can quickly pull up...the “Articles Citing this Plasmid” section. Having IDs linked to these metadata and publications help to...

...is proud to present our updated CRISPR Guide! The CRISPR Guide helps scientists understand how to use...incredible new CRISPR technologies developed and widely adopted in the past few years, as well as refreshing...refreshing the existing content. Access the CRISPR Guide here! Highlights include a much-anticipated prime... PRIME editing Please check out the updated guide for a great introduction to what CRISPR technologies...

... viral attachment and fusion. Once inside a host cell, new evidence in PLoS Pathogens shows that the Ebola...week within two months. That said, Ebola is not considered a “terribly infectious disease” – infection occurs...via body fluids not through the air and that’s considered unlikely to change – but the illness has been...thought we’d do a little digging and assemble a rough guide to the science of Ebola and related resources. Ebola...

...share ideas. Ideas had over lunch are just as valuable! In 2019, Addgene implemented a company-wide New ...Transferable Skills Guide: Problem-Solving Transferable Skills Guide: Teamwork Transferable Skills Guide: Identifying...are still struggling with idea generation, try this thought exercise: Consider the issue as if there were...faster that way— but the idea behind the study holds. To generate novel ideas, start with an out-of-the-box...most prominent idea, and instead building an environment where everyone has their ideas heard.  So how...in which the goal is to generate unique ideas. Recognize ideas can come any time. Meetings shouldn’t be...to encourage the sharing of ideas, not to give people more work. Identify your enemies In Felicia Day...

....    If this video is helpful, you can find our complete collection of protocol videos on the Addgene...antibody's purity? Check out our latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies...

...then, in having a guide that curates this information. And so I created the Codon Guide to Synthetic Biology...The video channels are also underrated and I recommend checking them out, especially since videos on synthetic...knowledge but little biology experience.) The Codon Guide to Synthetic Biology One of the best ways to learn...begin their journey into synthetic biology. The guide is a curated list of research papers, blogs, free...send me suggestions. The most useful parts of the Guide are probably the papers and textbooks. I’ve selected...to find. I hope that many people will find this guide useful as they start their synthetic biology journey...

...three or four guides directed towards that gene as there can be a large difference in guide potency. Thus...whichever guides are closest to the transcription start site (TSS). We recommend targeting the guides to a ...scientific world by storm. While Cas9 has been most widely used to create specific edits in DNA, there has...required for Cas9-based activation. CRISPRa for genome-wide studies One of the best uses for Cas9 activators...  Which Cas9 activator should I use? There are a wide variety of activators you can use for your experiments...transcription. That being said, we generally pick guides by putting the promoter of the gene into a gRNA...(2015) WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system. Genome Biol 16:...

... and adenoviruses in this easy-to-use guide. Print out the guide and use it for quick reference when you're...information on viral vectors? Check out our viral vector guide pages, our Lentivirus FAQ, and our viral service...pages. Click on the Image Above to Download the Guide ...

...alone. So, if you consider yourself part of the scientific community, get used to the idea of working on ...our kit process substantially and have plenty of ideas for further improvements. Types of teams There ...the first few meetings and we’ve volunteered our ideas, skills, and time to the cause ever since. Unlike... necessarily need a diverse group of people to provide that base. Sometimes there’s just too much work...ever taken has declared me an introvert, and I identify as one.  But some time around 10th grade, I snapped... a happier person. What does a good team need? Besides that all-important sense of purpose, there are ...multiple people: someone to coordinate, someone to decide, someone to delegate, etc. If you find that there...

...backgrounds. Sometimes this can lead to wildly different ideas of how to approach a problem, but it is these very...Addgene. Another advantage of Scrum is that it provides transparency to everyone involved about the status...serve Addgene and ourselves. We are also able to consider different technical approaches that may come up...from the user's perspective which in turn helps us decide how to best approach it in our code.  Sometimes...our knowledge, and love to collaborate, which, incidentally, are the same principles for open science. I...

...comprehensive guide to Conflict Resolution. There are countless classes and resources that can provide details...ScientistsTransferable Skills Guide: LeadershipTransferable Skills Guide: Teamwork  ...” in quotations here because I would not have considered myself neutral in any of their disagreements....open new career opportunities for you, if you can identify them in yourself and learn how to sell them. Types...decision making, and stalled projects. You want to identify and address these types of harmful conflict early...other parties, the first step I always take is to identify the real source of the conflict. It can be easy...important to retain the stories and examples that have guided your resolution processes in the past. Types of...

...what I had done in order to identify potential sources of error and then decide on a strategy to fix the ...from the transferable skills guide: Teamwork Collaborating with others outside of your organization Time...need some help. Today in our transferable skills guide: Problem-Solving. I don’t know about your experience...went wrong with a PCR is a pretty niche skill; outside of a lab, where is that going to come in handy?...has been invaluable in my more recent positions outside of the lab. Granted, I currently work on the Scientific...situation, come up with potential solutions, and decide on a course of action that effectively resolves...solving as a generalizable process.  Step One: Identify the problem and gather information to help you...

...college, I knew I wanted to try research, but had no idea what to expect. I had a lot of help from my peers...scientific endeavors. I hope that this post can provide some perspective and advice to any undergraduate...no biological or biomedical scientists and had no idea what the daily life of a scientist was like. The...importance of research in basic biology, medicine, epidemiology, public health, and more.     A few of the...professional training after earning a doctorate Considered a "stepping stone" position to become a professor...future. How do I get started with research? I had no idea what my scientific interests were or what I wanted...is good advice, and it is ultimately up to us to decide whether we want to take it or not. Choose wisely...

...dumpsters outside the open windows in the middle of your talk. Technical difficulties turn your slide deck ...interest incorrectly on slides 8-16. Your notes disappear. The wrong version of your slide deck gets uploaded...hard. Aside from the diving board charades, I doubt anyone but me remembers any of the incidents above–and...Addgene blog Transferable Skills Guide: Time Management Transferable Skills Guide: Conflict Resolution Transferable...stand up in front of an audience and convey your ideas clearly and strategically is powerful in almost ... allows you to share and build support for your ideas, invite others into decision making processes, and...help me achieve my purpose? Should I go over this slide again? Say, 'Oh, I forgot to mention X, so let's...