...offering temperature-sensitive materials, we noticed a large percentage of these shipments arrived in India at...expertise in a specific country can quickly reduce a large number of barriers. Though we haven’t solved every...

...reconstitution via mRNA trans-splicing. By splitting a large gene into smaller fragments, delivering them by ...prime editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various contexts...

...deliver larger cargo such as base editors and prime editors. While using AAV to deliver large transgenes... with split AAVs. In general split AAVs break a large transgene in two pieces and package each piece into...

...Reddit. Here are some reasons why: 1. Reddit has a large and active community of people interested in science... moderators did not delete or suppress were the large number of scientist posters with serious career ...

... to its efficient use in screening experiments; large amounts of virus are required to ensure that you...

...open-access. Though it is time for me to refill my large coffee cup and get back to our booth, I will have...

...colonies that are comprised of cells with a large nucleus, large nucleoli, and little cytoplasm. Positive...other methods. This could be a reflection of the large size of the plasmid or silencing of the plasmid ...oncogenes, such as Myc. Viral vectors also have the largest genomic footprint of iPSC generating methods due...infect dividing and nondividing cells and have a larger cloning capacity (8-10 kb) than other retroviral...transfection efficiency is cell-type dependent and larger plasmids have lower rates of transfection. Minicircles...

...destined to continue in academia. It seems it’s largely a matter of luck if a student or postdoc ends up...

...transformed without a heat-shock step. Because of the large number of transformations being performed at Addgene...

... possible to quickly and efficiently assemble a large number of building blocks with just two or three...

...individual plasmid within a single bacterial cell. Large plasmids usually have a low copy number (approximately... other hand, smaller plasmids can be present in large numbers, 50 or more per cell and have a high copy...usually want to start 2 mL in a falcon tube, but for larger preps you might want to use as much as a liter ...

...counter-selection. Anecdotally, there is also a large amount of user-to-user variability, and in some ...modification of bacterial genomes. It can accomplish large deletions, insertions, and even precise point mutations...

...companies now sell fast digest enzymes that can digest large amounts of DNA in as little as 10 minutes, but check...Additionally, if your final product is going to be very large (>10kb) you might want to use electro-competent ..., the DNA migrates toward the anode (+ charge). Larger fragments of linearized DNA migrate slower than...purification you can do is a miniprep, but if you need larger quantities of DNA, you’ll need to do a midiprep...

...genes for deoxyribose synthesis Allows uptake of large plasmids hee   High electroporation efficiency...Chloramphenicol High competency cloning and propagation of large plasmids, ligated DNA, and libraries, nalidixic ...

... from 10-50 µL depending on application and is largely determined by the volume of DNA to be cut. A typical...website about star activity . If you are digesting a large number of plasmids with the same enzyme(s) (for ...

... responsible for cancer from genomics data, and large-scale patient cohort data from the MD Anderson Cancer...

...less efficient at taking up larger plasmids. If you need to transform large plasmids, it is a good idea...very few if any transformants when transforming large plasmids (>10 kb) or BACs, what can I do? Chemically...

...1) other strategies may not be feasible and (2) large-scale proteomic and transcriptomic datasets already...CRISPR/Cas9. You check the mutation and it causes a large deletion that removes the entire exon that codes...

...migration resistance and improve separation. For large proteins, reduce the percentage of acrylamide. To... and typically takes less than an hour. However large proteins typically do not transfer as well using...

...mutations behind. Furthermore, piggyBac™ offers a large cargo-carrying capacity (over 200 kb has been demonstrated...recombinase. However, the use of negative selection largely overcomes this obstacle. Furthermore, we have found...a cell line with the edit of interest. With the larger piggyBac™ donor vector, it is possible to make ...