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We narrowed to 974 results for: LAT

Showing: 141 - 160 of 974 results

  1. Promega Plasmid Collection

    Type
    Collection
    ...monitoring of signaling pathways, investigating RNA regulation, tracking protein degradation, analyzing CRISPR-mediated...

  3. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...promoter, forms blue colonies on LB/Amp/Xgal/IPTG plate pAdx-CMV-copGFP 73346 Expresses copGFP from the ...

  4. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...acceptor molecules and can therefore be used to calculate the distances between the donor and acceptor. ...

  5. Optogenetics AAV Preps

    Type
    Collection
    ...Promoter CAG CaMKII CBA Dlx EF1a/nEF Synapsin E2 regulatory element Fluorophore GFP Red-wavelength fluorescent...

  6. Biosensor AAV Preps

    Type
    Collection
    ...CAG CaMKIIa Dlx EF1a GFAP/GfaABC1D Synapsin E2 regulatory element Activity Cre-dependent Flp-dependent ...

  7. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... Ca2+ channel phosphorylation site pS1928 [L132/18R] Cav1.2 Ca2+ channel phosphorylation site pS1928 Rat...

  8. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...Understanding intracellular pH regulation is important as pH regulation has roles in many cellular functions...the stop codon. Thus a non-translated mRNA will appear yellow, but a translated mRNA will be red. This system... were untranslated in the nucleus, confirming the hypothesis that most mRNAs are not translated until ...P-bodies are translationally repressed whereas the nonsequestered pool goes on to initiate translation. These...in separate subpopulations of mitochondria, one can monitor how the two separate populations move and interact...histidine kinase, which triggers phosphorylation of the response regulator and downstream transcription activation...particularly CRISPR), it is now relatively easy to design constructs and repair templates to insert TAP tag fusions...

  9. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...perpendicular lines that intersect your populations. If you see a diagonal population, this is a sign of spillover... this case, the population on the left is a horizontal line, so our circled population on the right needs... bleed in this population. Note that in the corrected flow plot in 2A, the populations are not perfectly...straight, but the center of each population is in a line with the other populations.   Figure 2: Compensation... move it (Figure 3). If the population is not a double-positive population and is instead due to bleeding...for both at similar levels, meaning that your populations will ‘look’ similar on your flow plot. This will... difficult to properly gate your desired cell population. By compensating between fluorescent channels...

  10. Working with Nuclear Receptors

    Type
    Blog Post
    ...complexes with secondary coregulator (or co-coregulator) partners. The coregulator ‘core’ complex is composed...illnesses, and the metabolic syndrome. Coregulators Coregulators constitute a group of some 400 molecules... www.nursa.org for a curated list of coregulators). Coregulators can function as coactivators and corepressors...loosely bound co-coregulators associate with the core complex in a dynamic, regulated manner. The fact...individually contribute to cell regulation in a coherent manner. Because coregulators exist as multiprotein complexes...with a systematic effort to compile NR- and coregulator-related global gene profiling datasets in to our ... curated NRs and coregulators. We look forward to growing this important relationship with Addgene for...

  11. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...looking at the different populations. In the case of Figure 2, one population is B- and another two are...Figure 2: This flow plot has two populations that are B+ and one population that is B-. Created with biorender.com... the gate is one population and everything outside the gate is a different population.  Gating and plot...above, until the population of interest has been successfully identified and isolated.  Following a gating...forming a population in the lower left(ish) corner.  If it’s a bit difficult to see these populations, I suggest...gated populations; this indicates the percentage of total events contained in the gated population. Heat...you’re interested in studying immunology or subpopulations of cells, you’ll soon find yourself encountering...

  12. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...system, which uses light to regulate transcription, also includes light-regulated histone methyltransferases... dCas9-Tet1 demethylates cytosines at promoters and enhancers. This targeted demethylation is associated... dCas9-DNMT3A methylates cytosines at promoters and enhancers. This targeted methylation is associated... Q147L mutation improves methylation kinetics such that cytosine methylation occurs within 24 hours rather...expression that go beyond genomic sequence. Dysregulation of the epigenome (the sum of epigenetic modifications...general, epigenetic chromatin modifications are correlated with alterations in gene expression, but causality...tools for this purpose. Epigenetics began as a correlative field in which covalent modifications to DNA ...

  13. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...amplifies the entire plasmid template. The parent template is removed using a methylation-dependent endonuclease...the PCR products, the plasmid template should be isolated from a methylation competent bacterial strain ...requires a template of high homology to the endogenous target, yet to facilitate the repair, the template should...duplications (slightly larger bands sizes relative to the original template). Due to the small difference in size...introduce or remove restriction sites), in mapping of regulatory elements (to mutate promoters/enhancers in reporter...resistant to Cas9 cleavage. If a plasmid contains the template, site-directed mutagenesis can be used to mutate...nicked plasmid (the PCR product). Plasmids are isolated from the resulting colonies, and screened for ...

  14. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ... same way, the GFP- population should overlap with the negative control population (Figure 1). Based on... experiments, these populations may overlap slightly, or the distinct populations may not be as obvious...expressed regularly in cells and might be upregulated or downregulated based on your experiment. You can see... densities, so signal shifts due to upregulation or downregulation are easier to identify by comparing...signal, or upregulation, and a peak to the left indicates a lower signal, or downregulation. Figure 3 ...results for your antibody of interest: two clear populations or a huge smear across your FSH vs reporter plot...acquisition, make sure that the signal for the positive population is no higher than 105 signal intensity. Signals...

  15. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...column, plate Mini, midi, maxi, mega, giga $ Yes —beads, columns, plates, buffers 96-well plates, 8-well...Beads, column, plate Mini, midi, maxi $ Yes — beads, columns, plates, buffers 96-well plates, bead kits, ... Column, plate Mini, midi, maxi, mega, giga $$ Yes — columns, plates, buffers 96-well plates, automation-friendly... column, plate Mini, midi, maxi, giga $ Yes — beads, columns, plates, buffers 96-well plates Not mentioned...Millipore Sigma Column, plate Mini, midi, maxi, mega, giga $$ No 96-well plates Some Endotoxin-free, BAC...Promega Beads, column, plate Mini, midi, maxi $$ Some — buffers 96-well plates, automation-friendly In...Fisher Beads, column, plate Mini, midi, maxi, mega, giga $ Some — buffers 96-well plates, automation-friendly...

  16. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...cloning and the FNLCR Combinatorial Cloning Platform (CCP); the latter of which greatly enhances the utility...assembly cloning platform called GMAP (Gibson assembly-based modular assembly platform). GMAP is based ... that locus to be methylated. This powerful tool has been used to direct methylation to and lower expression...method, called STARR-seq (self-transcribing active regulatory region sequencing) that tests enhancer sequences...enhancers function independently of their position relative to a transcription start site. In STARR-seq, randomly...researchers to screen the entire genome for genes that regulate a wide variety of phenotypes, including but not...the resulting lentiviral library to generate a population of HEK293FT cells expressing a single gRNA targeting...

  17. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...transfection into plates containing selection agent at different densities, then dispose of the plates that do ...) to transition from a 48-well cell plate to a 96-well assay plate. We generally freeze at least 2 ampules...rapidly isolate individual cell clones with different expression characteristics from a population of transfected...became frustrated with using glass cloning rings to isolate colonies of antibiotic-resistant cells; during ...cloning rings, the remainder of the colonies on the plate dried out and died. The alternative to cloning rings... rings, dilution cloning into 96-well plates, is extremely time- and resource-consumptive, since only ...cell can give rise to single clones, and thus many plates must be examined for single clones and then handled...

  18. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...technology can be used to isolate a specific subset of cells in a population in a procedure termed fluorescence...stream oscillates to generate droplets that are charged as they pass through a metal deflection plate. Each...lymphocytes and “gate” that population for further analysis. Since different populations of immune cells express...desired cell population for further analysis. In this example, the lymphocyte population is selected and...analyze a specific subset of cells in a complex population. Have you spent hours in the biosafety cabinet...cytometry allows users to analyze single cells in a population. Single cells are passed through the path of ...target protein expression levels, assessing post-translational modifications, determining cell health, analyzing...

  19. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...and negative populations. Oftentimes, you’ll be presented with a trend of two populations with basically...within a clear negative population. When you’re interested in measuring a population based on a fluorescent...two stains in relation to each other. Using a quadrant gate, I can divide my cell population into DN, DP...experiments you’ll be working with a heterogeneous cell population, for example from a processed piece of tissue...But even if you are analyzing a homogenous cell population in culture, it is normal to observe diversity...Figure 3 you can see how I gated out my lymphocyte population from a tumor sample. Many cell types can be found... I can however clearly identify my lymphocyte population (10.7% of all recorded events) based on their...

  20. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...occur directly after any 3’ regulatory elements, such as the polyadenylation or poly(A) signal. While many...purpose and mechanism of polyadenylation vary across cell types, but polyadenylation generally serves to promote...which promotes both polyadenylation and termination. Out of those listed, the SV40 late polyA and rbGlob ...export and translation processes as well.   Figure 2: The conserved eukaryotic polyadenylation signal...efficiency element of the simian virus 40 late polyadenylation signal by using in vitro analyses. Molecular...working with plasmids! What are termination and polyadenylation? The role of the terminator, a sequence-based...half-life, and ultimately gene-expression. Polyadenylation is the post-transcriptional additional of multiple...
Showing: 141 - 160 of 974 results