...adeno-associated viral vector (AAV) samples and created a simplified process, termed viral genome sequencing (VGS)...viral genome sequencing workflow. Using this simplified approach, we sequenced over 100 AAV vector preparations...routinely producing AAV. We believe that this simplified sequencing and analysis approach can be easily...

...researcher on parasitoid wasps; and an essay on the life of Srinivasa Ramanujan, to be precise) and remarking...neurological health risk. Having played the sport my entire life, I was curious as to what the research had to say...chatting with individuals whose research ranged from wildlife conservation to abortion law to poetry. A research...

...multiplex immunolabeling of brain. eLife 8:e43322. https://doi.org/10.7554/elife.43322 Bandrowski, AE, Martone...created by James Trimmer, PhD, of the University of California Davis School of Medicine. This deposit includes...

...which limits its use for imaging a protein’s entire life-cycle. This makes it nearly impossible for scientists...permanently attached to a protein, making them hard to amplify and image for long periods of time.  Are antibody...demonstrated that the HA frankenbody can be used to amplify fluorescent signals in in vivo imaging: the 10xHA...

...Individual Development Plan (IDP) and uses it to match qualified scientists to jobs in industry, academia, and ...find open positions and view their required qualifications and skill sets Taking the time to identify...Communication Networking As the number of qualified scientists entering the workforce increases, EAs...

...to target cells as a pooled library, which will amplify each TF to determine how its presence influences...processes. For help with library transformation and amplification, refer to the Zhang lab’s previously published...Addgene.org Plasmid Pooled Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome Library...

...multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:e43322. doi: 10.7554/eLife.43322. PMID: 30667360; PMCID...antibodies allows you to use them in parallel to help amplify your signal - giving you the benefit of a polyclonal...

...evidence (in psychology, cancer biology, pre-clinical life science work, economics) that published research... their study plans at a point in the research lifecycle when suggestions can be incorporated into the ...and as the Director of Advising in the Division of Life Sciences at Rutgers University. His dissertation...

...allowing the device to take on a community-driven life of its own. Nevertheless, there are still many open...Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying Quantitative Imaging Platform Development and... B., Marsikova, B. et al. Open microscopy in the life sciences: quo vadis?. Nat Methods 19, 1020–1025 ...

...with the desired mutation, then use those to PCR amplify the template plasmid. The parental, nonmutated ...primers, each containing the desired mutations, to amplify both strands of a plasmid template. In addition...pitfall of REPLACR-mutagenesis is that you must PCR amplify the whole plasmid, which could introduce mutations...

...strands take part in rolling circle DNA amplification. The amplified DNA segment is labeled with fluorescent...directed toward AHA. Now the rolling circle amplification could only take place if the two antibodies ...

...plasmid (Option 1) or added using PCR-based amplification (Option 2). Advantages of Golden Gate cloning...is to "domesticate" your fragment: PCR-based amplification can be used to create silent point mutations...high-efficiency step. References: Sequential amplification of cloned DNA as tandem multimers using class-IIS...

... I possess.   Bullet points on resumes are like cliff notes, so I use these as a starting place for drafting...technical and transferable skills that make you qualified for the position. Highlight the outcomes or deliverables... have made it to the interview if you weren’t qualified for the position. So prepare for the interview...

...antibody, a primary-secondary pair is used. This amplifies the signal as multiple secondary antibodies can...ELISA, the only difference being the additional amplification step, which helps to visualize low-abundance...DNA damage and instability detection. Cell. Mol. Life Sci. 76, 4689–4704 (2019). https://doi.org/10.1007...

... dozen versions of the toehold switches and amplification primers. This helped make sure that we had a...this includes primer directed isothermal RNA amplification, new toehold switch designs, CRISPR/Cas9-mediated...global environmental suitability for Zika virus." Elife 5 (2016): e15272. PubMed PMID: 27090089. Resources...

...work on the origin of cellular life. His lab has long hypothesized that life started with RNA and a simple...cautioned the distinction of “higher” and “lower” life forms as we shared millions of years of common ancestry...

...reduced their lifespan. Because these dramatic conditions were experienced in a period of life when the grandparents...statistically significantly associated with reduced life-span, but no changes in the DNA sequence could be...

... industry early would only help. Like any major life decision, the transition felt scary. While I knew...scientific discussion is often part of your daily life. Also, while you’re unlikely to be laid off as an...never-ending cycle of grant-writing that is academic life. Of course, because you will be part of a team in...

...reference sequence and the gene of interest to be amplified in the same reaction. Taqman’s increased sensitivity...of interest Some gene sequences are harder to amplify and quantify than others. If available, ddPCR may...chain reaction methods for the determination of amplification of Plasmodium falciparum multidrug resistance...

...controlled timing of CRISPR/Cas9 delivery. eLIFE. (2014) 10.7554/eLife.04766 Moreno-Mateos, M. A., Fernandez... timing of this approach carefully (Lin et al., eLife)! Use a Cas that facilitates HR What if promoting...