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Showing: 141 - 160 of 395 results

  2. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...repaired by base excision repair, but can lead to mutations in single stranded DNA undergoing replication....endA negative, meaning the gene has been removed/mutated to neutralize the nuclease activity. For strains...

  3. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...disease typically caused by a single R206H point mutation on the receptor ACVR1. ACVR1 triggers appropriate...regeneration in healthy patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous...

  4. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...the introduction of a mutation into the genome, how can cells with that mutation be selected/screened?...

  5. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...inactivates the gene and acts as a marker for the mutation. When designing these types of experiments it ...not to use the same resistance cassette for the mutation and for plasmid selection. Additionally, scientists...

  6. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ... Blog Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate Insertion ...

  8. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...toxin, indicating a 70% frequency of biallelic mutations. Importantly, hESCs retained their stemness, indicating... remove the risk of viral integration causing mutations and gene expression changes elsewhere in the genome...

  9. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...the preferred pathway for generating knock-in mutations, and NHEJ is an error-prone pathway frequently...frequently introducing frameshift knockout mutations. Thus, if genetic manipulation one way or the other (knock-in...

  11. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...enhancers and untranslated regions, or the effect of mutations of these regulatory elements on gene expression...expression. The regulatory element (wild-type or mutant) is cloned upstream of the luciferase gene in the plasmid...

  13. Hot Plasmids - May 2022

    Type
    Blog Post
    ...long sequences with reduced risks of unwanted mutations in mammalian cells. Long sequences traditionally...traditionally have higher risks of off-target mutations. These plasmids contain catalytically inactive dCas9 and...

  14. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...non-functional đť›˝-galactosidase. Complementing this mutation by expressing this deleted portion, for example...custom primers are especially helpful in verifying mutations that wouldn't be reached by sequencing from either...

  16. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...vector with half the size of the ssAAV genome has a mutation in the terminal resolution site (TRS) to form ...genome with wild-type ITRs at the both ends and mutated ITR at the center of symmetry. After uncoating ...

  19. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...gene. One such strain is DB3.1, which contains a mutant version of DNA gyrase (gyrA462) that is resistant... al. “F plasmid CcdB killer protein: ccdB gene mutants coding for non-cytotoxic proteins which retain ...

  20. Design Tips for Prime Editing

    Type
    Blog Post
    ...with BioRender.com. Add silent mutations near point mutations to create 3-base (or longer) tracts...
Showing: 141 - 160 of 395 results