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Showing: 141 - 160 of 391 results

  1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...modified monomeric red fluorescent protein (RFP), named GEMCherry2, that can rapidly and efficiently evaluate...methionine start site. This first iteration was named GEmCherry1, due to the “genomic fragment inserted...

  4. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...gut microbiota Monitoring microbial population dynamics in the gut can provide insight into how the microbiome...distributed cell division counter reveals growth dynamics in the gut microbiota." Nature communications ...

  5. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...needed steps to continue where the guide left off - namely, a blog post devoted to the other affinity reagents...Single-domain antibodies (sdAbs), sold under the commercial name nanobodies, are derived from a camelid antibody ...

  6. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...Note that many antibodies are sold under different names through multiple vendors, so look at epitope target... between the same antibody sold under different names.  If all else is equal, feel free to make the decision...

  7. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...Stanley Qi and used in Chen et al. to track telomere dynamics throughout the cell cycle. To optimize the signal...Addgene.   References 1. Chen, Baohui, et al. “Dynamic Imaging of Genomic Loci in Living Human Cells by...

  9. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...tags, and antibiotic resistance markers (just to name a few). In this edition, we’re going to take a look...genome manipulation. Cre recombinase, originally named because it “causes recombination” (although later...

  12. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...ligand co-developed by Bryan Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise...Nissen W, Pekcec A (2019) Pharmacokinetic and pharmacodynamic actions of clozapine-N-oxide, clozapine, and...

  13. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...articles published there as well.   Worm Development Dynamics Database (WDDD) The WDDD aims to acquire quantitative... Kyoda, Koji, et al. "WDDD: worm developmental dynamics database." Nucleic acids research 41.D1 (2012)...

  16. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...Mike Lacy In order to study the conformational dynamics of the motor protein kinesin-1, the Twelvetrees...single-molecule FRET to study the intramolecular dynamics of the proteins in solution and when associated...

  17. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...compensate between them. Another rule of thumb is if the names of the fluorescent markers are similar, you will...Chart.  Pro tip! Some antibodies go by different names depending on your flow cytometer’s setup. For instance...

  18. PhD Applications After COVID

    Type
    Blog Post
    ...program. Most applications will recommend that you name two professors in your application that you intend...reference your questions about the PI’s research or the name of the award you won for your conference presentation...

  19. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...want to include your university and your advisor’s name for some context and maybe common ground. The people...also helpful to write your Twitter handle on your nametag for the tweep up (and for the entire conference...

  20. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ... analyzed.   End point PCR End point PCR, as the name implies, analyzes the end product of PCR temperature...Quantification.”   Multiplex PCR Multiplex PCR, as the name implies, is a method in which multiple targets can...
Showing: 141 - 160 of 391 results