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We narrowed to 550 results for: NOL

Showing: 141 - 160 of 550 results

  1. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...Voigt: The top products out of biotechnology today are things like butanol - very simple molecules - a few...it, his lab at the Massachusetts Institute of Technology has been “working on new experimental and theoretical... collision that’s taking place between CRISPR technology and synthetic biology, the tools he’s making ...

  2. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...recombination. What is Cre-lox? The Cre-lox system is a technology that can be used to induce site-specific recombination... lethality. To facilitate the use of Cre-lox technology, transgenic mice have been constructed that express...system is another site-directed recombination technology very conceptually similar to Cre-lox, with flippase...analogous to Cre and loxP, respectively. The FLP-FRT technology can be an effective alternative to Cre-lox, and...

  3. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ... Potential Applications in Biotechnology. Critical Reviews in Biotechnology, 24(4), 155–208. https://doi.org...virulence plasmid are now used routinely in biotechnology to transform plant cells. Degradative plasmids...Nature’s toolbox for new and exciting molecular technologies!  This post was originally written by Jessica...

  4. Hot Plasmids February 2024

    Type
    Blog Post
    ...Susanna Stroik Even with the advances in CRISPR technology over the past decade, primary cells remain difficult...using peptide-assisted genome editing. Nature Biotechnology, 10.1038/s41587-023-01756-1. https://doi.org...monomeric StayGold fluorescent protein. Nature Biotechnology, 1–4. https://doi.org/10.1038/s41587-023-02018...throughout the non-human primate brain. Nature Nanotechnology, 18, 1241–1251 (2023). https://doi.org/10.1038...

  5. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    ...revolution for antibodies. Recombinant antibody technology makes it easy for scientists to share, remix,...proposed a shift to recombinant DNA-based antibody technologies (Bradbury & Plückthun, 2015a).   Figure...monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife 8: . https://doi.org/10.7554...srep31730 Tsuruta LR, dos ML, Moro AM (2018) Display Technologies for the Selection of Monoclonal Antibodies for...

  6. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...ideas (how to stop rhino horn poaching using biotechnology) were thrown on the table and dissected with...reputation. The public is can be fearful of this technology and angry at the agriculture companies that produce...opportunity has given us the chance to develop the technology we will need, and the business training to make...and Biochemistry from the Illinois Institute of Technology in 2004, she accepted a commission in the United...

  7. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...qPCR Using SYBR Green TechnologyAAV Titration by ddPCRAAV Purification by Iodixanol Gradient UltracentrifugationAAV...Figure 1: Overview of key steps of AAV production, iodixanol gradient purification, and AAV titration by qPCR...purified by density gradient centrifugation (e.g., iodixanol) using a high-speed ultracentrifuge. During the...

  8. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...Prime editing is a versatile genome editing technology that allows precise modifications of DNA (replacements...features and DNA repair determinants. Nature Biotechnology 2023, 1–11. https://doi.org/10.1038/s41587-023...and product purity by deep learning. Nature Biotechnology, 41, 1151–1159. https://doi.org/10.1038/s41587...efficiency across diverse chromatin contexts. Nature Biotechnology. https://doi.org/10.1038/s41587-024-02268-2 ...

  9. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ... last 5 years to incorporate more science and technology into the school curriculum. Our Parent Teacher...and the internet before it, genome engineering technology is moving along much faster than it can be officially...sugarcoat the potential dangers of people using the technology in an unethical manner, but I also tried not ... timely.” Genome engineering, and many other technologies and fields in biology, are moving so quickly...

  10. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...must stay current with literature and emerging technologies to remain competitive and relevant. Enter: the...department, we had two recurring journal clubs on Immunology and Microbial Pathogenesis that occurred once...spirited and focused as opposed to a wandering monologue?  Assuming the feedback is generally positive ... Will Arnold...

  11. CRISPR Antimicrobials

    Type
    Blog Post
    ...Figure 1). Citorik et al. targeted beta-lactam and quinolone resistance genes in E. coli using plasmid and ...plasmid. In the case of the genomically encoded quinolone resistance, mediated by a single base pair change...efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed: PMID 252409288...produce sequence-specific antimicrobials.” Nature Biotechnol. 32(11): 1146-50. (2014). PubMed: PMID 25282355...

  12. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ... for clinical diagnostics, genotyping, and biotechnological advancements. Many methods of nucleic acid...Biosciences has been started based on DETECTR technology with the mission to “provide a CRISPR-based platform...strip format. They published this in Nature Biotechnology. Like the SHERLOCK detection system, DETECTR...CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Chen...

  13. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...the AOX1 promoter whose expression is induced by methanol. Once linearized, the delivery vector can be integrated...Zeocin resistance. Then, counter-selection with methanol medium selects for those cells that have removed... In cells where recombination has not occured, methanol induces the production of the toxic MazF leading...Vibrio cholerae vaccine strain CVD 103-HgR." FEMS Immunology & Medical Microbiology 57.1 (2009): 69-79. PubMed...

  14. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...reports suggest rapidly freezing virus in a dry ice/ethanol bath or liquid nitrogen prior to storing. Know ...effects, detection, elimination, prevention.” Cytotechnology 39.2 (2002). 75-90. Pubmed PMID: 19003295. ...oncoretroviral and lentiviral gene therapy vectors.” Biotechnology Advances 24.3 (2006). 321-37. Pubmed PMID: 16448798... effect of sera and polycations.” Molecular Biotechnology 53.3 (2013). 308-14. Pubmed PMID: 22407723. ...

  15. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...it comes to altering gene expression, CRISPR technologies have gradually taken over as the preferred method... their promoters in human and mouse. Nature Biotechnology, 35(9), 872–878. https://doi.org/10.1038/nbt... constructing shRNA expression vectors. BMC Biotechnology, 6(1). https://doi.org/10.1186/1472-6750-6-1...mechanisms of actions, and circulation. Frontiers in Endocrinology, 9. https://doi.org/10.3389/fendo.2018.00402...

  16. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ..., 2013). APEX catalyzes the oxidation of biotin-phenol to the short-lived biotin-phenoxyl radical in the... 2012). It is important to note that the biotin-phenol reagent can be toxic to living cells thus APEX ...living cells and organisms with TurboID." Nature biotechnology 36.9 (2018): 880. PubMed PMID: 30125270. PubMed...encoded reporter for electron microscopy." Nature biotechnology 30.11 (2012): 1143. PubMed PMID: 23086203. PubMed...

  17. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...alternatives to restriction enzyme cloning. These newer technologies have become more and more common, and for good...Gibson assembly combined with the popular CRISPR technology and is described in a recent publication by the...technically challenging. Other homology based technologies We've described Sequence and Ligation Independent... Kahl, L. J. & Endy, D. A survey of enabling technologies in synthetic biology. J. Biol. Eng. 2013; 7,...

  18. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...from the lab bench into drug development. HiBiT technology helps close this gap! Bridging discovery gaps...  Figure 1: Schematic of HiBiT tagging technology.   The bioluminescence advantage There are...a closer look at several applications of this technology. In each section below, we cite the original ...LgBiT, and the authors used a p53 response-element NanoLuc reporter to show that the mutants abolished transcriptional...

  19. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...founded.  This commercialization of gene editing technology led to the development of many important medical...double-strand breaks (DSBs) in the genome. These technologies rely on the cellular DNA repair machinery, which...genetic engineering and open advertisement of the technology on various non-scientific platforms, CRISPR has...perspective of the current. Although genome editing technologies have brought a renaissance in the field of health...

  20. Typing CRISPR Systems

    Type
    Blog Post
    ...classification system for the array of CRISPR technologies. Learning the general principles of this classification...the first taught when introducing CRISPR as a technology. There are three subtypes, A–C, with the beloved...Y. (2018). Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing. Cell ...Yoshimi, K., & Mashimo, T. (2022b). Genome editing technology and applications with the type I CRISPR system...
Showing: 141 - 160 of 550 results