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We narrowed to 567 results for: PAC;

Showing: 141 - 160 of 567 results

  1. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...physical organization of your workspace and your workflow can have a huge impact. Test different approaches...in a pattern of small errors that significantly impacts your experiments. What do you do when you’re in... experiments at odd hours, used more secluded workspaces, or politely asked to not be disturbed when they...

  2. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...experiment. Here, we’ll discuss NHEJ, and how it impacts what happens to Cas9-mediated DSBs in the genome...active throughout the cell cycle and has a higher capacity for repair, as there is no requirement for a repair...chromosomes within a day. Another factor expected to impact repair is that the Cas9 protein doesn’t immediately...in determining how the structure of broken ends impacts how they are repaired. He works in Dale’s lab.  ...

  3. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...to envision that my research will have a direct impact on healthcare and the community. As a result, I...competitions everything is conceptual, and the team that packages its pitch the best, wins. He explained that entrepreneurs...works well, the idea is solid, and “the pitch is packaged to perfection”, seed money will hopefully be raised...to Find a Job in Biotech and Resume/CV tips - LabSpaces Blog Find a Buisness Plan Competition Good Relations...

  4. Lentiviral Prep Service

    Type
    Collection
    ...details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled CRISPR...lentivirus available from Addgene's viral vector packaging service. Browse constructs currently available... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled CRISPR...

  5. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...’t have to worry about having enough space! There’s enough space here at our new location to grow. We ...phase of the organization and the expansion of our impact, I’d like to take a moment and honor all of the... able to do good in the world. We are making an impact.  {% video_player "embed_player" overrideable=False...

  6. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...makes it easier to package for therapeutic delivery by AAV, which has strict capacity limitations. New ... RNA editing. Fast facts – PAM requirement: protospacer flanking sequence – A, U, or C. Best for: targeting...development and use. Fast facts – PAM requirement: protospacer flanking sequence – none. Best for: targeting...

  7. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...and chloramphenicol resistance pCRISPR: carries a spacer targeting the gene of interest and kanamycin resistance...template, and the locus cannot be recognized by the spacer-derived crRNA. However, if recombineering is unsuccessful...survive have inactivating mutations in the Cas9 or spacer sequence, and these rare events are easily detectable...its condensed design. For both systems, custom spacers/gRNAs are easily inserted using BbsI and Golden...proteins, and (4) the donor DNA. By using a multi-spacer CRISPR array, CRISPR-transposons can be multiplexed...

  8. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...structures, etc., as well as the presence of a Protospacer Adjacent Motif (PAM) sequence. If the gRNA sequence...and base editing applications, with high editing capacity in both contexts for NRN PAMs and to a lesser ...desired site does not have the same devastating impact on efficiency as 10-15 bps away does. This Cas ...Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science 361,1259-1262(2018). DOI:10.1126/science.aas9129...

  9. Control AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Controls Viral Vector Packaging Service: Control AAV Browse... Page . Don’t See What You’re Looking For? Our Packaged on Request service offers you even more options...Please note this does not guarantee viral vector packaging service, but lets us know what viruses would be...

  10. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ... Viral Vector Packaging Service Penn Vector Core Transfer Viral Vector Packaging Service: Penn Vector...Optogenetics Scott Sternson AV-1-20071P 20071-AAV1 pACAGW-ChR2-Venus-AAV Optogenetics Karel Svoboda AV-1-...pAAV-hsyn-Jaws-KGC-GFP-ER2 Optogenetics Ed Boyden AV-9-20071P 20071-AAV9 pACAGW-ChR2-Venus-AAV Optogenetics Karel Svoboda AV-9-...-tdtomato Scott Sternson AV-5-20071P 20071-AAV5 pACAGW-ChR2-Venus-AAV Karel Svoboda AV-5-20949P 20949-...

  11. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...adenoviral helper genes are supplied on two different packaging plasmids: the pHelper plasmid containing Ad E2A... your infectious titer, since it will have more impact on your experimental outcome and planning.  Both... of AAV-2 particles via a novel capsid ELISA: Packaging of genomes can limit production of recombinant...resources on the Addgene blog Parts of the AAV Packaging PlasmidParts of the AAV Transfer PlasmidAAV Variables...

  12. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...may take up precious space in viral transfer vectors with limited packaging capacity. Additionally, it may...particularly when only a portion of the plasmid is packaged for viral delivery, or the relative expression...

  13. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...lost during fixation with these chemicals, which impacts sample structure and could also cause your target...destroy it for others. That said, organic solvents’ impact on lipids means that they are able to permeabilize....   Figure 1: Choice of fixation method impacts antibody binding. Formaldehyde cross-links the ...detergent has different chemical properties that will impact the level of permeabilization and membrane integrity...

  14. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...variable chains are secreted into the periplasmic space to be oxidized and form disulfide bonds (Frenzel...does not, be sure to set the absorbance to 280 nm (Pace et al., 1995). You’ll also need to know the extinction...Aggregation and storage Aggregation of antibodies may impact their quality and efficacy (Wang et. al., 2018)...10.1002/bit.27776. Epub 2021 May 4. PMID: 33811659. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T. How to ...

  15. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...individual presynaptic terminals, which are jam packed with everything needed for synaptic transmission...?  ExM provides a straightforward answer to the space problem. Instead of imaging smaller, what if we ...the anchoring, digestion, and expansion steps can impact the integrity of different targets and labels. ...continuity can appear reduced in ExM, which could impact how you interpret a target’s structure. For example...

  16. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...vectors. To quantify this comparison, capsids were packed with a GFP transgene and GFP intensity and transduction...versus AAV-PHP.eB in the CNS. A GFP transgene was packaged into either an AAV-PHP.B or an AAV.PHP.eB capsid...versus AAV.PHP.S in the PNS. A GFP transgene was packaged into either an AAV.9 or an AAV.PHP.S capsid. Both...here or check out these protocols if you want to package your own virus. ...

  17. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ... plasmid sample enters Addgene the same way. A package is delivered by a mail courier and then the journey...    The plasmid's journey begins First, each package is opened and the contents are carefully inspected...deposits arrive from labs around the globe. The packages have spent days to weeks in transit and some have...barcode can store a lot of information in a small space and is ideal for tracking large collections. Many...

  18. Technical Design of a Western Blot

    Type
    Blog Post
    ...binding capacity and high protein retention. It comes in a variety of pore sizes and protein capacity, allowing...conjugate will have to be tested to ensure it doesn’t impact the antibody’s binding to your protein of interest... is a little quicker to use, with high binding capacity, though it has lower retention after binding and...10 minutes) and simple, utilizing individually packaged membrane ‘sandwiches’. They work well for proteins...

  19. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...the CNS by lentivirus, which has a larger packaging capacity than AAV. Such an approach was described ...should be screened for activity in vitro and then packaged into AAV for in vivo delivery. Multiplex gene ... also possible and is only constrained by AAV packaging limits (i.e., how many sgRNA expression cassettes...

  20. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ... target is present immediately upstream of a Protospacer Adjacent Motif (PAM), the sequence NGG.  If these... SpCas9 and its enhanced variants, eSpCas9 and HypaCas9, are great choices. Check out our SpCas9 containing...Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9 for site-specific modifications... within the editing window (bases 4-8 of the protospacer). While this is a small window, this system can...
Showing: 141 - 160 of 567 results