We narrowed to 943 results for: POR

... to cleave and activate a reporter. The detectable signal from this reporter can be measured and quantified...and a Csm6 specific reporter (in the same fluorescent channel as the Cas13 reporter), they could significantly...variants, Cas13, and Cas12a, to develop simple, portable, and inexpensive platforms to reliably detect ...method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2) (Gootenberg... termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR) (Chen et al., 2018). Both SHERLOCK and... SHERLOCK- Specific High Sensitivity Enzymatic Reporter UnLOCKING Cas13 (C2c2) was first identified in...molecules. In SHERLOCK a quenched fluorescent ssRNA reporter is added to the reaction. Cleavage of the quenchable...

... the 3’ UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains...removal of GFP from the TRICK reporter. Delayed translation is known to be important in development, and Halstead...proteins to allow translation to occur. For a TRICK reporter mRNA this displacement leads to a change in the...GFP protein that binds to the coding region of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) an RFP...translation, both GFP and RFP are bound to the reporter, causing it to appear yellow. The act of translation...of the mRNA, leaving only a red FP bound to the reporter. Because it contains a nuclear localization signal...copies of the FP-coat protein fusions to their reporter mRNAs. Using translation inhibitors cycloheximide...

...the promoter of the reporter gene to activate expression (Figure 2c). The reporter gene then allows detection...genes as reporters and some that repress instead of activate transcription. The HIS3 reporter system (...sequence (UAS) of its target gene, in this case a reporter gene (e.g. luciferase or GFP), regardless of whether...co-expressed in a yeast strain that also contains a reporter gene cloned downstream of a promoter containing...referred to as the “bait”, Figure 2b) cannot activate reporter gene expression on its own, but, if the two POI...Gal4 activator, bacterial LexA DBD and the lacZ reporter gene (3, 4), there are now many variations, including...to have Y2H systems that avoid transcriptional reporters altogether; In the split-luciferase system, intein-mediated...

...cuts to important research funding.  So when I saw that there was going to be a march in support of science...the rain, about 4,000 supporters of science showed up to march. We had full support of local law enforcement...proved to be the perfect opportunity to cement in my children's heads the importance of science and positively...seems to break everyday. Many researchers and supporters of science marched to advocate for scientist ...for science, and numerous other reasons. It is important to remember that we, scientists and non-scientists...°W – Vanessa Sung Hundreds of scientists and supporters in Montreal took to the streets on April 22 to...Albany, Rep. Paul Tonko also came and spoke in support of science - he's one of the few members of Congress...

...has dubbed these fusions LumiFluor Reporters. The LumiFluor reporter constructs were designed to be packaged... The researchers took a FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase...share your constructs from lab to lab. Promega supports the Addgene mission statement: Accelerate research...(FLuc) and Renilla (RLuc) luciferase. This is important not so much for how bright you can make a reaction...couples NLuc to fluorescent proteins to make better reporters and the second couples NLuc to fluorescent proteins...host cells. The fluorescent moiety of the BRET reporter was used to sort successfully infected/expressing...NanoLuc luciferase. Both GpNLuc and OgNLuc BRET reporters produced bright signals without exogenous excitation...

... to report mitochondrial turnover Mitochondrial health, transport and turnover are important features...genes, reporters, or other markers into a target genome. They did this by selecting the important elements... be imported to the mitochondrial matrix. Gottlieb’s lab has used an inducible MitoTimer reporter using...usefulness in cell culture to report changes in mitochondrial turnover and transport. pMitoTimer from Yan’s ...s lab, which is constitutively expressed, reports on the balance of biogenesis and mitochondrial degradation...mitochondria in the networks marks the differences in import of newly synthesized (green) protein and organelles...channelrhodopsins. CnChR1, nicknamed Chrimson, is the first reported yellow-peaked channelrhodopsin with a spectral...

...mentor Your manager should support you, just like you are supporting your report(s). This means that you ...unique skills and strengths (you will likely have reports with different levels of experience), find out ...how to prioritize but I  just added the “most important” tasks my schedule. This was bad for 2 reasons...tasks before I was a manager! Second, my direct report was not engaged because I only left him/her with...expectations, answer any questions, and (most importantly) decide on a timeline. My experience is that ...you’re delegating a recurring task to a seasoned report, there should be questions! Remember that delegating...develop your team members by giving them new opportunities. I still need to check myself sometimes to ...

...of the opportunities you’re provided throughout your PhD program.   Find people who will support your ...had a wonderfully supportive mentor and graduate office. I cannot stress how important they were to my ...experimenting with the career and networking opportunities available to me as a graduate student culminated...events for adults and professional development opportunities for researchers interested in connecting with...they had my back. If your advisors are not as supportive of your career goals, find people who are. I ...as types of experiments. I searched for new opportunities with an open mind and open ears. Everyone I ...mentioned, my graduate office staff was amazingly supportive. I talked to them about my interests and my ideas...

...everywhere can access plasmids despite the evolving export/import rules. Our shipping and lab teams continue to...entire supportive Board, we have a robust committee process in place and continue to incorporate additional...all of the success we have achieved and the opportunities we have for the future. At Addgene we will never... The success of these projects allows us to support more new projects. Diversification is happening... quality and success. Customer and technical support Our customer and technical service brands continue...sharing, but we don’t have to worry about getting support from depositors. We regularly hear how much they...blessed with a large community of scientists who support us by depositing, requesting and spreading the ...

...benefit from some Addgene support? Please get in touch, as we aim to support a couple of events a year...across Europe get introduced to the cultures and opportunities available to them at university. At a Freshers... into university life. The fairs are also an opportunity for the societies to promote their upcoming events...biological societies that Addgene has had the opportunity to work with at Freshers Fairs in the past year...undergraduates and PhD students so that they can help support each other and provide advice. The society organizes...involved with. The society provides talks and career support for a range of different scientific disciplines... in its first year. The society facilitates opportunities to learn about Synthetic Biology and exciting...

...et al. Cell. 2015. PubMed PMID: 26627737 pORTMAGE: A portable method for bacterial genome engineering ...-ome using a quick, affordable, and ubiquitous reporter assay. In their recent publication, Kroeze et ...their enhanced TANGO arrestin recruitment assay incorporating 314 codon-optimized GPCR sequences for human...MAGE uses recombineering (2) to simultaneously incorporate multiple single-strand DNA (ssDNA) oligonucleotides...so-called “genomically recoded organism” (3) but its portability to other bacterial strains remains seriously ...bacterial species (4). This set of plasmids (dubbed pORTMAGE) expresses the λ Red recombinase enzymes, as well...can be used in a broad range of strains. Thus, pORTMAGE simultaneously allows genome editing and mutant...

...plasmids, are export-controlled materials in the USA, where Addgene is located.  The export license, if ...requestors. We also offer export license applications for VSV and SARS-COV-1 plasmids. Export control license ...Addgene is excited to announce we now offer an export license application for rabies materials! Some ...license process    An export control license, once approved, allows Addgene to ship plasmids or viral vectors...the applicable research project when purchasing export-controlled materials. Addgene will then apply for...process.   Due to recent regulation changes, an export control license for rabies materials may be required...required for others. You will be notified if an export license is not required for your country after ...

...You need the support of your institute to do this one. Universities will usually only support tenure track...Further Evidence (RFE). Letters of support are the only opportunity that you are given to present your...and so on. Candidates should look broadly at opportunities to develop skills related to their areas of ...must have both substantial merit and national importance; (2) The foreign national must be well positioned...evaluating an NIW, USCIS will also focus on the importance of the individual’s work in their field and the...working under in the lab or has won, awards, media reports, and membership in groups related to the foreign...This criterion is best proven through letters of support from experts in the field. You must also be able...

...STEAMid. Internships open doors to a web of opportunities that the classroom cannot offer. Furthermore... not deter any student from applying to the opportunities of their dream.  To help students focus on the...below:  1. Eligibility check Scrutinize every opportunity prior to starting an application to assess your...unless you are applying for a Federal Government opportunity, which tends to be more detailed. Beyond the ...draft a personal statement, which is the perfect opportunity to paint a comprehensive picture of your academic...statement will pay dividends. This is the only opportunity where you get to impress the judges on your ability...your recommenders deliver a superb and detailed portrayal of yourself. Be mindful that January and February...

...molecular brightness (Table 1).  NIR reporters and biosensors NIR reporters can tell us about cellular events..., D. & Michnick, S.W. An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions...FPs) were derived from natural photoreceptors incorporating biliverdin IXα (BV) autocatalytically. Fortunately...should be supplied (Baloban et al., 2017).  It is important to consider effective brightness of NIR FPs in... a cell and depends on the efficiency of BV incorporation by an apoprotein and protein expression level...higher apparent cellular brightness than previously reported widely used dimeric iRFPs (Table 1). In general...Oliinyk et al., 2019). They were engineered to incorporate BV. Their advantages are their small size (17...

...it is incorporated. We were surprised to see how rapidly the efficiency of mutation incorporation falls...contains the intended sequence changes to be incorporated into the edited genome. In mammalian cells such...these two strategies allows you to specifically incorporate homo- or heterozygous mutations. 1. Increasing...therefore strongly encourage any researcher to incorporate CRISPR/Cas-blocking mutations at both targeted... our frustration) in many cases we only saw incorporation of the blocking mutation. Despite the fact that... away from the cut site were not efficiently incorporated during HDR. We decided to systematically characterize...only about 30 bp, it is no longer feasible to incorporate mutations without screening thousands of clones...

...emphasizes that reporting the sex of cells and tissues—when that information is reliable—is important to ensuring...Universities need to incorporate these methods into their curricula. Corporations need to integrate these...Governments, universities, and, increasingly, corporations in the US and Western Europe have taken three...into research. Why are sex and gender analysis important? Doing research wrong costs lives and money. According...positive, the analysis did find 263,000 more osteoporotic fractures. The overall economic savings to the... goal of gendered innovations It is crucially important to get the research right from the beginning. ...unique to male stem cells. Nor did they detect important differences in function between male and female...

...expression, sometimes incorporating chemically inducible approaches to provide temporal control, this new ...random variation, or noise, in reporter expression. To do so, one places reporter expression under the control...expression of a reporter protein, it is possible to change the amount of noise in reporter gene expression...can deliver these components together using electroporation. In these bulk cell experiments, Clift et al...no stress or damage upon TRIM-Away, and the electroporated cells re-adhered to the culture plate within...deliver the target antibody. TRIM-Away reagents and reporters are available from Addgene - we hope they will...lab has deposited plasmids encoding fluorescent reporters and other proteins controlled by GAL4 and ZIF268...

...gene. Spatio-temporal expression of optogenetic reporters: Since most optogenetic reporters are delivered...invert the reporter and GeneR fragment. Then the second recombination event would excise the reporter from ...transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic reporter would ...Cre-dependent reporter expression, the potential for background expression of the reporter will be greatly...shown in Figure 2, the cassette should contain a reporter DNA sequence (such as GFP) in the sense orientation...the two pairs of target sites. By flanking the reporter with one loxP site and one lox511 site in the ...would ensure that the reporter remains silent until a cell or tissue-specific Cre is provided. Since the FLEx...

...Figure 1). Addition of substrate generates a proportionate luminescent signal, strong enough to detect ...signal to gradually diminish over time; and, importantly, repeated excitation generating reactive oxygen...targets in translational research Researchers can incorporate HiBiT with a wide range of workflows, from classic...paper for more detail on whether the authors incorporated HiBiT by CRISPR tagging at the endogenous locus...the authors used a p53 response-element NanoLuc reporter to show that the mutants abolished transcriptional...editors Tracking factors that utilize exosomal transport is quite challenging. Work by Shibata et al. (...internalized. Functional assays further showed that imported ADAR1 increased oncogenic editing of its most ...