...    Figure 4: (A) Using the proportional relationship between FSC-H and FSC-A we can gate on single...displaying your events. You are free to choose any size-related parameter, such as FSC-H, FSC-A, SSC-H, SSC-A, ...Figure 6: (A) A flow plot showing two stains in relation to each other. Using a quadrant gate, I can divide...

... model organism for scientists as they have a relatively short generation time for mammals - the time ...that are laid externally. Fruit flies are also relatively straightforward to manipulate genetically. Scientists... zebrafish reference genome sequence and its relationship to the human genome." Nature496.7446 (2013):...

... design and engineering of fusion proteins is relatively straightforward. However, generating a fusion...fluorescent protein. Thus, scientist began using relatively long linkers between the protein of interest ...C-terminal residues of the fluorescent protein are relatively close together (figure 1). Where should the fluorescent...

...Also, in cases where the protein of interest is relatively small, adding GFP may alter diffusion rates. ...Although bioluminescent probes are usually dim relative to fluorescent probes, recent engineering efforts... that the N- and C-terminus of GFP itself are relatively close, thereby minimizing the disruption of the...

...post-translational modifications, quantify protein levels relative to standards, detect the cellular location of ...thickness of the band provides information about the relative abundance of the protein in the sample. More protein...molecular biology protocols Browse other articles related to antibodies Resources on Addgene.org Find written...

..., 2005; Deisseroth et al., 2006). Despite its relatively recent development, the history of optogenetics...delivery in the central nervous system due to their relatively stable extra-chromosomally long-term expression... neuronal inputs to induce aversion- or reward-related behaviors. The high spatio-temporal precision and...

... day or a week planning an experiment, even a relatively simple experiment. However, by taking this time...surprising findings when studying a seemingly unrelated problem. However, you have finite time and resources...satisfying. Finally, many PFs are in committed relationships and/or have started a family. Those people are...

... give advice and guidance to those that ask. Relationship building is key in science policy. Luckily, ...happy hours that you can attend to help build relationships.  Reach out to a science policy professional..., communications, diplomacy and international relations, advocacy, and writing legislation (see Figure...

...sites with no cross-talk. These pairs are closely related to the lambda phage integrase system which is the...intracellular pH are required in order to determine the relationships between pH changes and cellular events. To overcome...promoter-selection marker or promoter-inducible expression related vectors are also ready for further chaining. Plasmids...out there (and particularly CRISPR), it is now relatively easy to design constructs and repair templates... the efficiency of their cell-free system in correlation to the size of the product. Analysis of protein...

...scatter (FSC) and provides information about the relative size of the cell; larger cells produce more FSC...intensity of events. Since intensity positively correlates with expression, the further the shift of a cell...experiment, you would need a way to determine the relative contribution of yellow and orange from FITC and...

...Cervical Cancer is the leading cause of cancer related morbidity and death among females in Ghana and ...for cervical cancer development and also better correlate the presence of certain HPV subtypes with risk...

...the order they appear in the plasmid and their relative position to the insert. Example, if you have a... immediately followed by its position (number) relative to the start Methionine (Met) followed by the ...

...assemble a rough guide to the science of Ebola and related resources. Ebola Basics A few basics: Ebolavirus...of Medicine have similarly curated their Ebola-related content. -And the latest updates on the ongoing...

...attracting and keeping diverse talent in science related careers. Failure to strengthen the talent pipeline...science career resources Browse all blog posts related to science careers Download the Science Career ...

...fleeting. “What’s been shown by others is that, in a relatively short amount of time, cancers become resistant... library to include activators for more cancer-related pathways and more nodes within those pathways. ...

...up hobbies (for her its gardening) that help you relax and enjoy life. “I love academics because it offers...other's accomplishments? Any tips on making time to relax and/or enjoy time away from the bench? We would ...

...mentors and mentees have fulfilling, productive relationships. I will be sharing what I’ve learned along the...many had examples of very productive mentoring relationships with advisors and others who helped shape their...

...Offices at universities to make COVID-19 research-related plasmids available more broadly: to scientists ...resources on the Addgene blog Browse blog posts related to COVID-19 Learn more about the Ginkgo COVID-19...

...person, and helps improve your interpersonal relationships with people you’re collaborating with remotely...external collaborations Browse our science career related blog posts ...

...opportunities for investigating genetic mechanisms related to morphogenesis, cell fate specification, and ...activity on NAG PAMS and reduced activity on NGG PAMs relative to wild type. A new genome-wide CRISPR knockout...