... 100-fold excess of wild-type bacteria and the relative frequency of each of the 40 library strains was...

...agarose gel to confirm product presence, size, and relative quantity. End point PCR is most commonly used ...reagents and several heating and cooling steps. PCR relies on heat resistant DNA polymerase from the thermophilic...

...While this method can be time-consuming, it is relatively effective in reducing off-target effects since...

...Ras researchers, one of the most exciting and relatively new resources is the RAS Pathway Clone Collection...cases arise and millions of people die from cancer-related complications each year. Despite these grim statistics...making the results of any such study as clinically relevant as possible. Connecting the research community... initiative to curate our collection of cancer-related plasmids such that each is linked to a known major...

...future edits. In contrast, since Cpf1 cleaves relatively far away from the PAM, NHEJ might retain the ... genetic capacity of ~4.5 kb. At 4.1 kb, SpCas9 barely fits into AAV when you add in a gRNA and regulatory...

...Nicotiana benthamiana. N. benthamiana is a tobacco relative and a good model for plant disease because it ...tools work in any plant? Patron: The MoClo kit is entirely for dicots, not grasses and wheat, but we do work... have your parts. That was the motivation for releasing a large parts kit. Once you have so many parts...

...practically this isolation is only feasible for relatively short DNA molecules. In order to isolate a particular...most plasmids are replicated in E. coli and are relatively small (∼3000 - 6000 basepairs) to enable easy...Because bacteria are easy to grow in a lab, divide relatively quickly, and exhibit exponential growth rates..., and thus the plasmid exists in a symbiotic relationship with the host cell. Like the bacterial chromosomal...recommend checking out these two Addgene blog posts relating to common E. coli lab strains and E. coli strains...specialized for protein expression for additional strain-related information and a more extensive strain list. Strain...endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 tonA JM109 Addgene; Promega e14-(McrA-) recA1 endA1...

... all the proteins in the class that showed the relative sizes and the conserved domains. This information... maybe you’re researching a topic for which no relevant or recent review exists. Once you pick a topic...format also has the benefit of exposing you to relevant researchers in the field. Finally, you can sound...from start to finish. Find the information that’s relevant to you, note it, and move on. At the beginning...

...molecular mechanism, I was careful to keep it relatively simple. I honed the complexity of the talk by...rather “let’s see how we can make this fun activity relate to STEM.” Figure 1 shows the activities we had ...

...and uncertainty in estimating parameters from relatively sparse data from China. An important additional...particular scientific subfields.  Plasmid requests correlate with paper publication The dataset includes 11,426...

... other Cas effectors can’t, while maintaining relatively high efficiency. These alterations are achieved...inversions and large deletions. Many of these methods rely on Cas9 or a derivative of Cas9, but the ever-expanding...

.... Time Viral production in mammalian cells is relatively quick. After transfection, HEK-293T cells start... cells, and its lifecycle ends in cell lysis, releasing the virus to the supernatant. The virus will reach...

... some people still use amp? Carb is expensive (relative to amp), and for some applications, the benefit...their mode of action and have a very similar relationship as amp and carb (they are interchangeable). ...low rate, there is evidence that zeocin is never entirely detoxified, even when Sh ble is expressed (Trastoy...

...easiest switch from fluorescent proteins given the relative simplicity of the system and the availability ...

... use next-generation sequencing to measure the relative proportion of guide RNAs (gRNAs) across the population...die or differentiate Step 3 reveals which NANOG-related genes are critical to stem cell survival. Knowing...

... deliver a large amount of DNA (~8 kb) with a relatively low immune response, it should come as no surprise... co-transfection of these plasmids, 293T cells released transgene-containing lentiviral particles into...

... necessary control elements for each would be relatively large. Cloning the viral genes in this plasmid...

...future edits. In contrast, since Cpf1 cleaves relatively far away from the PAM, NHEJ might retain the ...

...and purification An affinity tag, generally a relatively small sequence of amino acids, is basically a... Thus far Plasmids 101 has covered GFP and its related fluorescent proteins, which are sometimes used ...crystallizing it or testing its function. Small ubiquitin-related modifier (SUMO) can help with folding and stabilization...western blots, IP, co-IP, IF, flow -cytometry, but rarely used for purification as elution requires low pH...  0.8 Affinity and Purification Very small size, rarely affects function S-tag KETAAAKFERQHMDS  1.8 Solubility...

...creating and editing Wikipedia pages, OWW will be relatively easy for you to begin using. For the novice, ...and bacterial transformation. And more plasmid-related educational resources can be found in our Plasmids...the repository. These protocols show up on the related plasmid pages and can help scientists use these...