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We narrowed to 601 results for: Ret;

Showing: 141 - 160 of 601 results

  1. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...with a mix of VSV-G and the baboon endogenous retrovirus Rless glycoprotein (BaEVRless) had high rates...polymer. Polybrene has long been used to increase retroviral transduction efficiency by neutralizing the charge...the cell surface, and also helps form DNA and retroviral-particle or VLPs complexes. The team found that...

  2. CRISPR in the Clinic

    Type
    Blog Post
    ...alternatively be harvested, edited ex vivo, and then returned to the patient. Finally, allogenic therapies involve...approach, editing patient cells ex vivo and then returning them to the patient. The first human trial involving...clinical trial for SCD in 2022 as well. Hereditary transthyretin amyloidosis (hATTR) hATTR is caused by a variety...

  3. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED TO DEMONSTRATE...cleaves relatively far away from the PAM, NHEJ might retain the PAM site. Therefore, if HDR does not initially... FJ, Terns RM, Terns MP, White MF, Yakunin AF, Garrett RA, van der Oost J, Backofen R, Koonin EV. An updated...

  4. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...optimist, I’m a realist, and together we make a pretty good team. We have confidence that we can take ...to a SynBio startup At some point, our ideas got pretty good. We started doing extracurricular research... successful business that the learning curve is pretty steep. But we can rely on one another to celebrate...

  5. Promega Plasmid Collection

    Type
    Collection
    ...interaction, association, and dissociation. NanoBRET Assays NanoBRET (Link opens in a new window) is an energy...proximity in living cells. NanoBRET PPI assays use NanoLuc Luciferase as a BRET energy donor and HaloTag...window) and NanoBRET assays (Link opens in a new window) NanoLuc®, HaloTag®, NanoBiT®, and NanoBRET® are Promega...Promega collection are available in two formats: pretransformed in bacteria as an agar stab 20 µg purified ...HaloTag protein, labeled with the NanoBRET 618 fluorophore, as the energy acceptor. Live-cell reagents allow... time using full-length proteins or fragments. NanoBRET target engagement (TE) assays (Link opens in a...compounds is measured by competitive displacement of a NanoBRET tracer, reversibly bound to a NanoLuc luciferase-fusion...

  6. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...pipetting, as shown in the YouTube video. Before returning the plate to the incubator, additional growth ...fluorescent protein), fluorescence microscopy can theoretically be used to select specific wells from the 48...SH-SY5Y cells, as these cells did not seem to form discrete colonies but constantly migrated away from each...

  8. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Plasmid Description Industry Return to top Blue Plasmid Description Industry Return to top Cyan Plasmid Description...Description Industry Return to top Green Plasmid Description Industry Return to top mEmerald Plasmid Description...Description Industry Return to top Yellow Plasmid Description Industry Return to top Orange Plasmid Description...Description Industry Return to top Red Plasmid Description Industry Return to top Photoactivatable Plasmid...Plasmid Description Industry Return to top Content last reviewed: 25 September 2025...

  9. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ... Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to top Drosophila...Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Publication Return to top Xenopus ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection...

  10. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...protocols, and more. Selecting crispr in Guides will return links including our CRISPR guide.  For a closer...What type of experiment are you planning? (e.g. Retroviral) Species: Specify the source organism for a gene... plasmids. A search for “RNA editing” plasmids returns more than 1300 results, and applying a filter for...

  11. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...review what exactly you are measuring and how to interpret your results.  Quick note before jumping in — ...average out noisy measurements. Be cautious when interpreting purity ratios, as they can vary significantly..., routine machine maintenance, and critical interpretation of the results.  If you see evidence of contamination...

  12. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones...Zebrafish Plasmids and Resources collection page Return to top Epitope Tag or Fusion Protein Tags and fusion...bacterial expression (Gateway) pYD1 - Expression, secretion, and display of proteins on the extracellular ...as TEV cleavable N-terminal His and NusA fusions Return to top Function Besides functions such as protein...expression, see our dedicated Lentiviral Plasmids page Retroviral Easy and safe to use, integrate into host genome...MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian retroviral gene expression with GFP or mCherry selectable...mammalian cells with the Tet-On or Tet-Off system Return to top Selectable Markers Regardless of your delivery...

  13. Zhang Lab CRISPR Page

    Type
    Collection
    ...nickase) and single guide RNA 48137 : PX165; SpCas9 Return to top GeCKO Screening Library GeCKO are pooled...for more information. Full references are below. Return to top Cas9 SAM transcriptional activation plasmids...sequences Zhang Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome Editing The Zhang Lab...fusion facilitates FACS sorting of cells and nuclei. Return to top AAV - Cas9 mouse CRISPR-Cas9 is a versatile...designed based on the target site sequence (20bp). Return to top References SpCas9 plasmids Multiplex Genome...CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini ...10.1038/nprot.2013.143. Epub 2013 Oct 24. PubMed . Return to SpCas9 plasmids GeCKO Library Genome-scale CRISPR-Cas9...

  14. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...developed a split intein spCas9-AAV toolbox that retains the gene-targeting capabilities of full-length ...10.1038/nmeth.3993  Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini ...Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated...

  15. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent...goal, what you expect to find, and how you will interpret and use the information. In contrast, a list of...

  16. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...2014, the White House issued a memorandum to the Secretary of Labor to update the FLSA, which had not seen...automatically adjusted every 3 years. In May 2016, Secretary of Labor Thomas Perez updated the overtime regulations...post by Director of the NIH Francis Collins and Secretary of Labor Thomas Perez, Fair Pay for Postdocs: ...

  17. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...trolling: The art of deliberately, cleverly, and secretly pissing people off, usually via the internet, ...time to start answering questions. r/Science has pretty strict rules about not being too sales-y in posts...questions from the AMA - as you’ll see below, it pretty nicely displays her energetic, truthful and helpful...

  18. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...support, but there are eight other teams who still retain a customer support element amongst their other ...The first and foremost sub-skill to have here is pretty intuitive: You need to be willing to face conflict...this skill to another job, it will be important to retain the stories and examples that have guided your ...

  19. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    ...get them to work on DNA instead. However, they retained a small amount of RNA-editing activity, which ...reduced RNA editing to background levels while retaining acceptable on-target DNA editing activity. In ...References Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W.,...

  20. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ... (a British idiom for “taking by surprise”) by Margaret Robinson’s lab, and more recently used by Stephen...Opto-RTK constructs – Opto-mFGFR1, Opto-hEGFR, and Opto-hRET – as well as various LOV-domain-mVenus constructs...directionally assemble in a single reaction, while retaining only a defined 4bp fusion site in between. The...vectors with the desired sequence of interest are retained after SLIC cloning. The general cloning strategy...
Showing: 141 - 160 of 601 results