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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 399 results for: SEF

Showing: 141 - 160 of 399 results

  1. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...inducible promoters. Inducible promoters are extremely useful if a desired protein product is toxic to cells ... with labelled or modified amino acids that are useful in different downstream applications. Cell-free...

  4. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...about these two new AAV capsids and how they can be useful tools for studying the CNS and PNS! What is the...PNS (figure32, tables 1 & 2). These capsids are useful tools for neuronal tracing and morphology studies...

  5. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ... Low Combines both words and images; especially useful for conveying and contextualizing data. Often overlooked...limited to a single page of information. Primarily useful for data-focused stories. In-person lectures...

  8. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... to bind and cleave target RNAs, although its usefulness in bacterial cells will likely be limited by ...molecule (like dCas9) suggests that dCas13a may be useful for isolating specific RNA sequences from a population...

  10. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ... hold large amounts of data. Although it may be useful to selectively bring particular items into an ELN... from repositories back into ELNs would also be useful, so that the data can be viewed – and conceivably...

  11. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...bind a variety of PAM sequences, which makes them useful when no suitable SpCas9 PAM sequence is present... have other characteristics that make them more useful than SpCas9. For example, Cas9 from Staphylococcus...

  12. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ... we’ve got you covered! Here you will find some useful tips to help you make your plasmid construction...easy-to-understand guided manner. These videos can be especially useful when designing new primers to clone your sequence...

  13. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ... alongside these antibiotics just fine. This is useful for keeping your eukaryotic cell cultures bacteria-free...resistance to selection toxins. Auxotrophic selection is useful for producing stable mammalian cell lines, but ...

  15. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...either a frameshift or 21+ bp indel. This score is a useful measure to determine how many of the contributing...either a frameshift or 21+ bp indel. This score is a useful measure for those who are interested in understanding...

  16. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...information from a variety of fields in order to provide useful solutions to complex challenges. As a cross-disciplinary...attention for their work, a video abstract may be a useful tool for turning video views into online article...

  17. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...your work out to other scientists who will find it useful, I highly recommend doing an AMA! Before I turn...have a lot of links and content at hand in three useful places: I had posted content on many of the requested...

  18. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ... the three toxins so that at least one might be useful in a broad array of pathogenic Proteobacteria. ...: AEM-00990. PubMed PMID: 31201277. Liljeruhm, Josefine, et al. "Engineering a palette of eukaryotic chromoproteins...

  20. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...something that has fallen out of fashion but is still useful and most importantly, effective. In the research... for gene silencing. RNA interference RNAi is a useful tool to investigate the roles of specific genes...
Showing: 141 - 160 of 399 results