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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 613 results for: SET

Showing: 141 - 160 of 613 results

  1. Pipetting Protocol

    Type
    Protocol
    ...Without moving past the maximum possible setting on the pipette, set the desired volume of the liquid on the...tip is properly set on the pipette to ensure pipetting accuracy. Once the pipette is set and you have the... pipette tip, the volume display which shows the set volume for the the pipette, and the tip ejector ring...

  2. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...published papers that contain that term. You can also set up alerts for new publications from a particular ...similar to Facebook or Instagram. Key features Setting up a personalized feed is simple. You can enter...

  3. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...options depending on above selections) Module set (the set of components you want in your vectors) Target... to switch out reporters, Cas proteins, guide cassettes, promoters, N’ and/or C’ terminus, and selection...destination vectors contain an ampicillin resistance cassette and an origin of replication, which are not schematized...

  6. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...follow-up DNA oligos to arrive. We also were careful to set up clear tasks for each member of the team to accomplish... can detect the virus in a clinically relevant setting. Molecular diagnostics for the Zika virus predominantly... the work done as quickly as we could. From the outset, we spread out the risk for the project by initially...

  7. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ... as the overall alignment results. The default setting is “Optimize for Highly similar sequences (megablast... it saves analysis time to remove this default setting. Timesaving Tip #2: blastx searches are inherently...

  8. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...structural plasticity in single dendritic spines. The set of CyRFP constructs available at Addgene includes...illustrates the utility of PhoCl in a variety of settings. For instance, inserting PhoCl in between a protein...molecular weight markers The Tan lab at Penn State - set out to “rule” Molecular Biology. Under the supervision... of Minnesota recently deposited a comprehensive set of plasmids that can be used for either TALEN or ...kanamycin cassettes and were carefully designed to allow for easy removal of the resistance cassette via Cre...Every quarter we highlight a subset of the new plasmids in the repository through our hot plasmids articles...the transcription factors from a polycistronic cassette. However, it was unknown whether variations in...

  10. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...PCR machine. Set annealing temperature 5°C below the primer melting temperature (Tm). Set extension step...create thousands of identical copies using a simple set of reagents and a basic heating and cooling (denaturing...choosing primers. Place thin-walled PCR tubes on ice Set up a 50 μL reaction (Keep all your reagents on ice...designed to have similar melting temperatures (Tm). Set the annealing temperature to 5°C lower than the Tm...MgCl 2 and/or 1µl DMSO to each reaction. The ideal setup for this troubleshooting step is to do one reaction...

  11. Plasmids for Stem Cell Research

    Type
    Collection
    ...Hochedlinger MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS cells...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes. piggyBac transposition reprograms fibroblasts...

  12. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...or "Unpublished or pre-publication plasmids" and set a desired distribution status. You can submit "Published... below:   Pooled Library A pooled library is a set of plasmids all built with the same backbone and ...entered will be saved and the page will open a new set of entry fields to describe the next insert. Repeat...

  13. Transferable Skills: Negotiation

    Type
    Blog Post
    ...variety of transferable skills. At Addgene, we've set out to fill this gap by highlighting that researchers...professional negotiation look like? In professional settings, creative negotiation skills are key to maintaining...get what I want, that means you don’t. In this mindset, one of us wins, the other loses. Although this...

  14. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...applications. These PCR methods all use the same basic PCR set up and steps but differ in how the PCR products are...amplified using several forward and reverse primer sets.  Multiple template PCR - multiple templates with...

  16. PhD Applications After COVID

    Type
    Blog Post
    ... of the environment of the department, the lab setting, and how students truly feel about the program ...stressful time, and I was more relaxed during both sets of interviews. I also had universities change their...

  19. Call for Guest Bloggers

    Type
    Blog Post
    ...have skill in a particular technique? Do you have a set of tips you give to every new lab mate? Do you have...

  20. Lab to Office Culture Shock

    Type
    Blog Post
    ...that can be difficult to replicate in other work settings: The sense of being the first to discover something...academia provide a formal training regime and the right set of tools to help you do your job as well as possible... find the idea of not holding a pipette again upsetting, there are ways to get back to the bench during...
Showing: 141 - 160 of 613 results