We narrowed to 179 results for: SPEN;

...convenient and fast way to deactivate genes without spending months generating mutants. Unexpectedly, phenotypes...

...board before or while the committee is arriving. Spend as much time as possible facing the committee and...

...seen with the naked eye. In 2023 alone, viewers spent over 22,000 hours watching these and other videos...

... pellets containing viral particles are then resuspended in PBS, aliquoted, frozen, and stored at -80 ...

...implicated in human diseases. His spare time is spent trying to raise the alpha isoform of GSK-3 to the...

...thought would be a couple months. Jennifer and I spent about an hour that morning scrambling. Everything...

...support teams behind them.  Conclusion Researchers spend 40% of their time on tedious administrative tasks...

...various research applications, they have become indispensable tools for manipulating cells and organisms. ...

...strength, while W results from the time that the cell spent passing through the laser beam. A is simply the ...

...them demand more services based on what they're spending. Increase the number of science masters programs...

...Transposagen. As a gene editing enthusiast, he has spent over 6 years in the lab developing and using a variety...

...? And boy did I make the right decision. If I’d spent my senior year isolating pheromones from various...

...academia is the uncertainty of it all. Though I had spent the first few decades of my life following a very...

...transfection, add csRNP and ssODN to the cell suspension, transfect and let cells recover for 2-5 days.Note...

...2009. 19(4): p. 429-38. PubMed PMID: 19255591. 17. Spence, J.R., et al., Directed differentiation of human...

... your project and not general opinion. I could spend a lot of time running through each tool in detail...

... Fluorescent imaging techniques have become indispensable tools for molecular and cell biologists over...

...20 min. Resuspend dry DNA with TE (10 mM Tris-HCl pH 8, 0.1 mM EDTA). Pro-Tip DNA resuspension can take...culture of bacteria transformed with your plasmid Resuspension buffer Denaturing solution Renaturing solution...several tubes/bottles. Remove the supernatant and resuspend the bacteria in buffer. Note: This step gets all... all of the bacteria back into suspension, but within a smaller volume of buffer that is compatible with...next solution. Add a denaturing solution to the resuspended bacteria. Note: This step causes the bacteria...column with 70% ethanol to remove excess salt. Resuspend the DNA pellet, or elute the DNA off of the column...Prepare the following solutions: Solution I - Resuspension Buffer 25 mM Tris-HCl (pH 8) 50 mM glucose 10...

...release the liquid. Pro-Tip Dispense until the first stop, wait, and then dispense to the second stop to expel...selecting the correct pipettor and pipette tip, how to dispense liquids, and how to handle the pipette. Protocols... use a pipette in many scenarios to accurately dispense small amounts of liquid (think: 0.1 µL to 1 mL...mL). When working in a laboratory, properly dispensing liquid ensures the accuracy of your experiment ...experiment and any changes to the amount you are dispensing can negatively impact your experimental results. This...The table shows the pipette with the appropriate dispense volume: This image shows the P2, P10, P20, P100...and P1000 as shown from left to right. Pipette Dispense Volume P2 0.2 to 2 µL P10 1 to 10 µL P20 2 to ...

...Pipet back and forth to resuspend each pellet completely. Combine resuspended pellets and keep on ice....Pipet back and forth to resuspend each pellet completely. Combine all resuspended cell pellets and sonicate...containing the resuspended virus from step 12 above. Add 50 units of benzonase per mL of viral suspension (you ...cells after 30 passages. Procedure Trypsinize and resuspend the HEK293T cells from 2 x T-175 flasks. Cells...vigorously multiple times to obtain a single cell suspension (no clumps of cells). Pool cells from 2 x T-175... Return to incubator for 48 h. Trypsinize and resuspend cells from the CS2. Cells should be at ~80% confluence...forth or shake vigorously to obtain a single cell suspension (no clumps). Count cells using a hemocytometer...