...spaced CRISPR software submissions. With few prior publications to go on, a submitted manuscript could only...few moments after the piñata burst, value was subjectively ascribed relative to the basic needs of each...software design that is applicable to only one, or a subset of organisms. In only a few cases the software ... agnostic. Most tools are exclusive to one or a subset of organisms. Examples: “organism agnostic”, “model... you can rule out any tool that limits you to a subset of specific genomes. These are most of the tools...

...Did you know that one of the quickest ways to submit deposits with more than 10 plasmids is to use our...the page, you’ll see two options for data entry: “Submit Online Deposit” and “Download Deposit Spreadsheet...Figure 1: Begin the deposit process by clicking “Submit Online Deposit”or “Download Deposit Spreadsheet...Plasmid sequences or GenBank files. We encourage submission of QUEEN-generated GenBank files (Mori and Yachie...Download a new spreadsheet each time you want to submit new plasmids! We update the spreadsheet every so...

...they are looking for in grant submissions and will direct you on how to submit your work either through seminars...writing and hopefully increase your success rate. 1. Submit your grant early! First off, and perhaps the most...for you to make the relevant alterations before submitting - handing them your application 3 days before...their scoring system or meeting with their grant submission panel. Some funding bodies provide opportunities...

...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...distribution of clusters without accumulation in subcellular compartments such as nuclear speckles. Furthermore..., by fusing a fragment of the kainate receptor subunit to the C-terminus of the protein. Unlike the diffuse...construct in which TagRFP-T and Dronpa flanked a PKA substrate fused to the phosphopeptide binding domain FHA.... The FHA1 domain binds to phosphorylated PKA substrate, inducing a conformational change that placesTagRFP-T...

...The blood-brain-barrier (BBB) exists to prevent substances in the blood, like proteins and viruses, from...mechanism unknown)   Chen et al., 2023 AAV9.452sub.LUNG1 Lung: ATII cells Mice N/A Goertsen... encompasses more molecularly-defined neuronal subtypes and with less bias among them than AAV-PHP.eB ...type II cell transduction in the rodent lung AAV9.452sub.LUNG1 is an engineered capsid derived from AAV9 ... does express in the brain. Both AAV9 and AAV9.452sub.LUNG1 have significantly higher off-target expression... validation, validation! (psst… don't forget to submit your validation data to our Data Hub once you’re...2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron,109(9), 1449–1464.e13...

...variety of topics. Individual forums are hosted on subreddits (r/”topic”) which are essentially lists of forums...Software for Biology) on Reddit and got some substantial traffic that we thought it would be a good idea...engaged, and has great questions! 2. The r/Science subreddit is well moderated. One of our original worries..., and even coached us on how to write our AMA submission to avoid rousing the trolls. 3. The AMA process...organized. You can check out the r/Science AMA Submission Guidelines here, but essentially we just needed...

...After production, all virus preps are titered and subjected to quality control by Addgene before being distributed...VP2, and VP3. Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed...Silver staining of purified and non-purified AAV subjected to gel electrophoresis. Viral capsid proteins ...lentiviral preparations (described above) that are subject to a concentration step prior to being frozen. ...gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for normalization purposes...

...chemiluminescent substrates are available. Labs with limited equipment often opt for the chromogenic substrates since...For enzymatic signal detection, users provide a substrate that the enzyme converts into a detectable product...antibody. The reporter reacts with a provided substrate and produces a signal that is directly proportional...enzymatic reaction between the detection antibody and substrate is proportional to the amount of antigen present...

...negative control is a sample or group that is not subjected to the experimental condition. In the example ...control could be a third group of mice that is subjected to the experimental protocol but does not receive...knockout.  If knockout samples are unavailable, try substituting with knockdown approaches using siRNA or shRNA...either by modifying the blocking conditions or by substituting for a more specific secondary antibody. The ...Immunoblotting and Immunohistochemistry: A Guide for Submission to the British Journal of Pharmacology.” British...

...or more copies of the same plasmid (Bedbrook & Ausubel, 1976). This recombination event produces a multimeric...therefore run higher on an agarose gel (Bedbrook & Ausubel, 1976). In fact, if your plasmid sample has a mix...the formation of plasmid multimers (Bedbrook & Ausubel, 1976; Fishel et al., 1981). E. coli strains such...independently increase the frequency of base substitution in human cells. J Biol Chem. 2011 Mar 25;286....1021/bi00850a028. PMID: 4878700. Bedbrook JR, Ausubel FM. Recombination between bacterial plasmids leading...

...identified subpopulation. For traditional cell culture, enriching your active RNAi subpopulation can be easily...necessarily dictates the direction and scope of the subsequent two. Consequently, I will focus on different ...gene silencing efficacy, thereby requiring a substantial time investment. Once the target mRNA is cleaved...and visual screening method was limited in two substantial ways: variable silencing efficiency and labor-intensive...

...same bottle of serum again and again for each subculture can enhance the growth of mycoplasmas. The serum...generated will enter the culture medium during subculturing processes or from the air if the cell line remains...source of the problem is the media, used for subculturing, which is available in both liquid and powdered...contamination present in the serum to be used for subculturing. Avoid using expired products or reagents that...viability of cryopreserved cells decreases with every subculture. Equipment Fumigate the laminar airflow and...

...itself. Many thanks to our guest blogger Subhadra Jayaraman! Subhadra Jayaraman is currently a doctoral candidate...This post was contributed by guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University... ability to inhibit further cancer growth and subjecting patients to the risk of microbial infection. ...cytotoxic effector T cells, and targeting specific DC subsets in vivo using specific antigens to activate them...

... or does not result in a reward sensation and subsequent repeated stimulation. A spatial memory task, ...activation of which results in depolarization and subsequent firing of an action potential. Halorhodopsins... of which results in efflux of chloride ions, subsequent hyperpolarization, and inhibition of action potential...help restrict expression to a specific neuronal subtype. However, the length of promoter that can be used...identification of the bregma and lambda sutures and subsequent determination of the proper coordinates of your...

... lines when annotating a cell line Community submissions via email: [email protected] Entries include... lines is included when available, and can be submitted via email for cell lines profiled in the lab. ...available information about a cell line you’ve used? Submit your manuscript or data to Cellosaurus via cellosaurus...sib.swiss.This community resource is actively encouraging submissions from scientists all over globe, with some surprising...

... biological processes. Chemical effectors are suboptimal for a number of reasons, including their potential..., promoting transcription (see figure below). Subsequent exposure to red light rapidly deactivates the...computational problem of edge detection. Tabor et al. subsequently engineered a second photosensitive TCS activated...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates the...

... Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...to studying membranes, organelles, and other subcellular compartments is via proteomics--the study of ...promiscuous labeling enzyme and a small-molecule substrate to map the spatiotemporal distribution and interaction...sequencing, revealing that they bind to a much larger subset of protein motifs than previously thought. Sidhu...

...your experiment. To make an FMO sample, you take a subset of your experimental samples, combine it into one...be from the same host species, have the same Ig subclass, and be in the same fluorophore (e.g., if you ...staining for anti-mouse Ly6C in APC with the IgG2c subclass, you will use the APC IgG2c isotype control). ...a FMO sample: you make a sample from a combined subset of your experimental samples, stain it with every...

...empirically determined. In addition, some metal ion substrates, such as those containing Co2+, have a lower ...affinity for polyhistidine than more commonly used substrates such as Ni2+ and are therefore more selective... systems, consider using these more selective substrates along with stringent wash conditions.   Find ...tightly the protein will bind to the metal ion substrate. If your protein of interest is expressed at a...

...Applications (RFA), what next? First, check the submission deadline and try not to panic if it's due in ...in mind that universities require application submission in-house approximately 2 weeks before the RFA..., award information, eligibility information, submission information, selection criteria, and award administration...written grant application builds upon the last submission. Thus, continuing to apply for funding improves...