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Showing: 141 - 160 of 303 results
  1. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...pustular psoriasis is a chronic autoinflammatory skin disease estimated to affect 0.27 to 4.6 per 10,000...Keratinocytes are the main source of IL-36 cytokines in the skin making it important to study what is happening within...
  2. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...inviting someone to connect, perhaps you should be asking yourself if it’s really a good idea.  Pro tip: ...accomplishments in both the technical and “plus skills” areas. Include a professional picture. It doesn...
  3. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...and use them as-is, saving time and effort but risking the potential of them not working, or spend a few... expected module order. (It’s also why you can’t skip a module entirely, but instead will have to replace...
  4. Engaging with science and society at pgEd

    Type
    Blog Post
    ...also beneficial to hone your non-technical writing skills by writing for science-outreach organizations or...work to a non-technical audience is an important skill for every scientist. After all, scientific research...
  5. Kit Free RNA Extraction

    Type
    Protocol
    ...was adapted from (Link opens in a new window) Chomczynski P and Sacchi N, 2006 and (Link opens in a new...
  6. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S. E., Muller, A.,...Low, S. H., François, A., Suárez-Amarán, L., & Samulski, R. J. (2022). AAV vectors: The Rubik’s cube of...
  7. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...poster or talk at the conference, you can end by asking them to come to your poster or talk. After the ...Volunteer at a professional organization to expand your skill set Resources on the Addgene website Become of...
  8. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ... take a lazier approach for harvesting my AAV. I skip the PEG precipitation of the HEK293 cell culture.... 2. Choi, V.W., Asokan, A., Haberman, R.A., & Samulski, R.J. (2007). Production of recombinant adeno-...
  9. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...Results from a survey conducted by Addgene in 2021 asking scientists what data would improve our AAV Data... your data free. Nature, 5–7. O’Connor, D. M., Lutomski, C., Jarrold, M. F., Boulis, N. M., & Donsante...
  10. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...Grades K-5 cover a broad range of attention spans, skill levels, and STEM exposure. If you expect a lot of...children but are interested in testing your SciComm skills or are interested in a career in education. Maybe...
  11. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ... References and Resources References  Özcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, ...Matthew T. N. Yarnall, Cian Schmitt-Ulms, Rohan N. Krajeski, Justin Lim, Lukas Villiger, Wenyuan Zhou, Kaiyi...
  12. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...chemical composition of the membrane itself? The Baskin Lab previously published OptoPLD, an optogenetically....2015.08.005.  Tei, R., Bagde, S. R., Fromme, J. C., & Baskin, J. M. (2023). Activity-based directed evolution...
  13. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...designed with the help of the DNA tuner program) (Latynski and Valentovich 2014). We routinely clone the ...e83. PubMed PMID: 25800749. PMCID: PMC4513841. Latynski US, Valentovich LN. "DNA tuner: a computer program...
  14. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...fed a diet of primarily egg whites would develop skin lesions and other terrible health issues. This disease...PMC3223083. 6. McInnes, J. L., Forster, A. C., Skingle, D. C., & Symons, R. H. “Preparation and uses of...
Showing: 141 - 160 of 303 results