We narrowed to 220 results for: Ung;

1. Hot Plasmids and Viral Preps - March 2021

   Type

   Blog Post

   ... markers. New CRISPR pooled library from Keith Joung’s and Benjamin Kleinstiver’s labs are now available...

2. 5 Tips for Troubleshooting Viral Transductions

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   ...Murnane, John P., Michael J. Yezzi, and Barbara R. Young. "Recombination events during integration of transfected...

3. Cpf1 Update: Comparison to Cas9 and NgAgo

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   Blog Post

   ...Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F. ...

4. Illuminating Epigenetics with A FRET Based Biosensor

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   Blog Post

   ...disabilities, problem behaviors, and a chronic feeling of hunger that can lead to excessive eating and obesity. ...

5. CRISPR Plasmids - C. elegans

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   ... In vitro transcription none, need Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection...

6. The AAVantages of AAV in CRISPR Screens

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   Blog Post

   ...Chamberlain, J. S., Corey, D. P., Aryee, M. J., Joung, J. K., Breakefield, X. O., Maguire, C. A., & György...

7. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   Blog Post

   ... S., Min, S., Bae, J. Y., Choi, J. W., Park, J., Jung, D., Yoon, S., & Kim, H. H. (2019). SpCas9 activity...

8. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   Blog Post

   ... Center did to create a model of Eml4–Alk-driven lung cancer (Maddalo et al., 2014). These tools are available...

9. PCR Applications for Copy Number Variation Assays

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   Blog Post

   ...10.1586/14737159.5.2.209. PMID: 15833050. Ma L, Chung WK. Quantitative analysis of copy number variants...

10. 3D Printing Meets CRISPR Cas9

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    Blog Post

    ...non-target DNA strand and shows the HNH nuclease domain swung around in close juxtaposition to its cleavage site...

11. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    ...-assisted insertion tagging), developed by the Hornung Lab, allows users to easily create C-terminal tag...

12. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    Blog Post

    ...females because you can be certain that they are too young to mate. Using various markers (more on that below...

13. Plasmids 101: Degron Tags

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    Blog Post

    ...13, 5495 (2022). doi:10.1038/s41467-022-33246-4 Chung HK, Jacobs CL, Huo Y, Yang J, Krumm SA, Plemper ...

14. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    Blog Post

    ...Central PMCID: PMC4686367. 2. Jaenisch, R. and R. Young, Stem cells, the molecular circuitry of pluripotency...

15. Light Sheet Fluorescence Microscopy

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    Blog Post

    ... Nature Protocols, 7(11), 1983–1995. Pubmed. K. Chung, J. Wallace, S.Y. Kim, S. Kalyanasundaram, A.S. ...

16. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post

    ...adapted to work in prokaryotic bacteria, not in fungal systems or plants. We are really plugging into ...

17. Synthetic Biology - Overview

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    ...Model Organism Bacterial Plant Yeast Mammalian Worm Fungal Algal Featured SynBio Deposits Alper Lab Y. Lipolytica...

18. AAV for Neuronal Tracing

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    ...DC, Barnard RJ, Mori T, Finke S, Conzelmann KK, Young JA, Callaway EM. 2007b. Monosynaptic restriction...

19. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    Blog Post

    ...pdb.prot076497. https://doi.org/10.1101/pdb.prot076497 Kim Y, Jung JP, Pack C-G, Huh W-K (2018) Global analysis of ...

20. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post

    ...ubiquitous promoter to express GFP in a group of young rats. We found that, in this case, AAV1 (23%) and...