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We narrowed to 581 results for: Vit

Showing: 141 - 160 of 581 results

  1. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...workplace is inevitable, but having who can help resolve these conflicts is not inevitable. When you know...operations and keeping our customers happy, but inevitably there will be gray areas that either a) overlap...meet. Most people can’t maintain that level of positivity past the age of five. But it does mean that even...competing, or accommodating, if you find yourself gravitating most frequently towards these approaches, you...

  2. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...displays enhanced brightness, expression, and sensitivity compared with earlier SF-iGluSnFR. Image from... response kinetics and increased brightness, sensitivity, and photostability under two-photon illumination...signaling receptors, but the options for measuring activity of specific RTKs are limited. In a new preprint...plasmids here!   Torres Cabán CC, et al. Tuning the Sensitivity of Genetically Encoded Fluorescent Potassium ...LiLac has improved response size and reduced sensitivity to pH and calcium compared to previous lactate...

  3. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...few minutes, I just need to split some cells.” Inevitably, this task would take longer than I thought, ... myself flexibility by never placing too many activities too close to one another. Monitoring task progress...) and, at the outset of a task, I outline the activities that are needed to accomplish these goals. Practically... of each day to remind myself of the specific activities that I need to work on. Throughout the day, I...during the times you’ve blocked out for other activities. This is also part of why I do much of my writing...

  4. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...for with in vitro display methods such as phage display (Bradbury et al., 2011). In vitro selection avoids...sequences are expressed as scFvs and screened with in vitro assays to select for strong binders. Then the sequences...2011) Beyond natural antibodies: the power of in vitro display technologies. Nat Biotechnol 29:245–254 ...of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced...

  5. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...phage display assays, ubiquitination activity assays, toxin activity, small molecule binding, growth rate...discover unexpected epistatic changes in protein activity. See Natarajan et al for an example of this. ...Error-Prone PCR”. Methods in Molecular Biology In Vitro Mutagenesis Protocols, pg. 103-109. PubMed PMID:.... Stephany, and Stanley Fields. “Measuring the Activity of Protein Variants on a Large Scale Using Deep...

  6. Viral Vector Guides and Plasmids

    Type
    Collection
    ...and envelope plasmids). When transfected into in vitro cell cultures, these plasmids direct the production...

  7. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    ...the Mushroom Kingdom. Kravitz Lab 2019 Holiday Door is up and running! @LexKravitz pic.twitter.com/J5bjHQo565...TannevanderWal) December 18, 2019   Holiday door The Kravitz Lab went all out to deck their door. What a fun... December 3, 2019 Hope you’ve enjoyed the festivities in the last month!  ...

  8. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...concentrations are low, pADH1 displays low promoter activity (~20% activity of the strong yeast promoter pTEF). As ...enabling it to bind the promoter and repress pADH1 activity. Researchers have engineered pADH1 variants that...“middle” ADH1 promoter pADH1m, which maintains activity during the late ethanol consumption phase of yeast...is active. In this way, you can interrogate the activity of uncharacterized promoters. Similarly, placing...

  9. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...this post: Glutamate indicators with increased sensitivity and tailored deactivation rates Addgene's own...interneurons Glutamate indicators with increased sensitivity and tailored deactivation rates By Mike LacyGlutamate...significantly improved brightness, kinetics, and sensitivity. iGluSnFR4f is well-suited for imaging rapid ... (2025). Glutamate indicators with increased sensitivity and tailored deactivation rates. bioRxiv, 2025.03.20.643984...). Reprogramming site-specific retrotransposon activity to new DNA sites. Nature. doi: https://doi.org...

  10. mTOR Pathway

    Type
    Collection
    ...tumor suppressor p53 also promotes aberrant mTORC1 activity, and multiple familial cancers are caused by inactivating...

  11. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... and two mutations in Cas9 to enhance nuclease activity; can be used in PE2–PE5 systems PE6 - small prime...

  12. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...enzyme that has been mutated to block its catalytic activity and instead become trapped in a covalent bond ...specificity for their own ligands with minimal cross-reactivity. Other strategies include using engineered ligases...2). If you’re working with purified proteins in vitro, you can even use the same tag for multiple proteins... actin filament assembly-regulating proteins in vitro reveals complex events. a) Micrographs of a fluorescently-labeled...Vivo Covalent TMP-Tag Based on Proximity-Induced Reactivity. ACS Chemical Biology, 4(7), 547–556. https:/...

  13. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...further rounds of RNAi. This process, known as “transitivity,” calls into question any claims about specificity...specificity of a given RNAi technique. While transitivity enhances the potency of RNAi, it can also increase...transgene, followed by a fluorescence readout of RNAi activity. As a plant researcher, the traditional hpRNA ...Mueller WF, Clauder-Münster S, Eberhard D, Fälth Savitski M, Grandi P, Jakob P, Michon A-M, Sun H, Tessmer... W (2019) Biological plasticity rescues target activity in CRISPR knock outs. Nat Methods 16:1087–1093...

  14. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...agility as a nonprofit resource can prove to be vital during this period of constrained funding. Addgene... even more valuable as labs look to maximize productivity and efficiency with fewer resources. Fostering...community has demonstrated remarkable resilience and creativity in the face of funding challenges. By strengthening...benefit of everyone. We Want to Hear from You We invite researchers to share how these funding changes ...

  15. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...of plasmid vectors and/or rely on cumbersome in vitro synthesis of single guide RNAs (sgRNAs), are time-consuming...tracrRNA system is much more potent than the in vitro-transcribed single guide RNA (IVT sgRNA) approach...Direct comparison of Cas9 RNPs prepared with an in vitro-transcribed (IVT) sgRNA versus synthetic cr:tracrRNA...efficiently and serves as a good control for both the activity of CRISPR reagents and ICC antibody specificity...of the crRNA (aim for 40-60% GC content) crRNA activity Local chromatin structure (although we could successfully...

  16. Promega Plasmid Collection

    Type
    Collection
    ... measure subtle expression changes with high sensitivity. These assays are ideal for real-time monitoring...

  17. AAV Packaged on Request

    Type
    Collection
    ...is used in a wide range of experiments, both in vitro and in vivo. It has low immunogenicity, is easy ...

  18. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    ...especially when done in vivo. There are also sensitivity issues when attempting to characterize mutations... the need for a faster and higher-throughput in vitro approach. Using data from The Cancer Genome Atlas...them had not yet been functionally validated. In vitro assay allows first-time functional annotation of...collection to screen individual perturbations in vitro using Ba/F3 and MCF10A cellular models. Ba/F3 and...

  19. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... H+ specific response cytoplasmic retention insensitivity to other ions In BRET, A mutant Renilla luciferase...mutagenized and screened for speed and voltage sensitivity. This hierarchical screen led to the generation...QuasAr2 possess mutations for improved voltage sensitivity and speed, and an endoplasmic reticulum export...construct, named CheRiff, exhibits high light sensitivity and fast kinetics. By combining both the voltage...produced luciferase is truncated and lacks enzymatic activity. The Luc plasmid (intronless) is useful for counter-screening...vector that enables protein expression in any in vitro translation system. The gene set was carefully chosen...the study of promoters with either weak or high activities (Bert, et al. 2000). To design pXPG, the authors...

  20. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...been removed/mutated to neutralize the nuclease activity. For strains that still carry this gene, there... purification protocol to remove endonucleases activity. For these reasons choosing the correct solution...any divalent cations like Mg2+, preventing the activity of DNase. While this is very important to preserve...temperature or at 4°C. Storing DNA: temperature and longevity You should consider a couple of more things when...
Showing: 141 - 160 of 581 results