We narrowed to 223 results for: antibody
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TypeBlog Post...catalogue numbers and manufacturers’ details for antibodies, plasmids (like your Addgene plasmid #), and ...an identifier (a bit like a GenBank ID) to all antibodies, animal models, and software tools in the Materials...
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Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog Post...who have complaints about the unreliability of antibodies. IPI is working to address this issue directly...directly by creating well characterised antibodies against all human extracellular proteins with community-...research tools. BenchSci collates information about antibodies while FPbase curates information about fluorescent... -
CRISPR Plasmids - Purify Genomic Loci
TypeCollection...can be used for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved by fusing... -
Twenty Years of Sharing Science!
TypeBlog Post...viral vectors, and in 2022 we added ready-to-use antibodies. Now, we think of ourselves as a repository for... -
Plasmid Collections
TypeCollection...γ-Retrovirus Adenovirus All Viral Plasmids Curated Topics Antibody Plasmid Collection Bacterial Expression Systems... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...are easily studied in living cells using antibodies. Antibodies could further be used to interfere with...with the identified surface proteins, and such antibodies could thus be interesting drugs. Known drug targets... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...riboprobe binding (via hybridization) specific antibodies that detect the mRNA-riboprobe complex are added...stain the cells that contain this mRNA-riboprobe-antibody complex and, as a result, these cells turn blue... -
Resolute Plasmid Collection
TypeCollection... production is used for assays and to generate antibody/ high-affinity ligands. Data is embedded in an... -
Addgene Protocols Go Viral: AAV Purification and Titration
TypeBlog Post...scientists produce vital tools in the lab such as antibodies or viruses. If you’re new to tissue culture, ... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...activity (Ni, Titov, & Zhang, 2006), quantifying antibody/antigen interactions (Saraheimo et al., 2013),...synthesized with reagents used to fluorescently label antibodies, and purification is a snap using thin layer ... et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A New Serodiagnostic Concept." PloS ... -
Plasmids 101: Degron Tags
TypeBlog Post...with the most alternative functions – there are antibodies to it and ways to achieve fluorescence with it...Plasmids for Endogenous Gene Tagging in Human Cells Antibodies 101: Epitope Tags ... -
SARS-CoV-2 Pseudotyped Virus
TypeCollection...Addgene Blog Posts Viral Vectors 101: Pseudotyping Antibody Neutralization Response Against Pseudoviruses ... -
Hot Plasmids - August 2020
TypeBlog Post... The benefits of nanobodies over conventional antibodies can’t be overlooked. Nanobodies are smaller in...easier to clone, express, and purify compared to antibodies. Nanobodies are easily expressed in the cytosol... -
Career Insights: Technical Support Specialist
TypeBlog Post...additional data regarding the specificity of this antibody?” You will also help troubleshoot products, give... in a Western Blot protocol and then blame the antibody when no signals are obtained. Or someone may heat... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...protein for which there are good flow cytometric antibodies, downstream of the TUJ1 coding sequence. ES cells...types are used. The lack of good flow cytometric antibodies for many CNS-resident cell types (microglia are... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog Post...Fluorogen activating proteins (FAPs) – These modified antibody fragments bind and enhance small molecule fluorogens...Nevertheless, these fluorophores were designed as antibody and oligonucleotide labels for fixed-cell imaging... -
A Scientist's Guide to Ebola
TypeBlog Post...component of vaccines and the target of neutralizing antibodies and inhibitors of attachment and fusion. Recommended... -
Antibody Guide
TypeGuide...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production... produce antibodies, with the highest variability in antibodies produced. Cells - Antibodies can also ...called recombinant antibodies. Recombinant antibodies are created by cloning antibody genes from specific... in a buffer. Antibody Structure Figure 1: Structure of an antibody A standard antibody is made up of ...specificity in antibody-based methods. Antibodies can recognize and bind other antibodies using epitopes...per antibody unit, which is typically the number of antibodies multiplied by two, as each antibody has...These are antibody mimics instead of modified antibodies and are not closely related to antibodies. Monobodies... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post...elucidate these interactions scientists have long used antibody based affinity purification and mass spectrometry...proteins, which can be a challenge for classic antibody based affinity purification/mass spectrometry ... -
Addgene Stands with the Scientific Community
TypeBlog Post...DNA sequencing, viral vector preparations, and antibody production that labs may find increasingly difficult...