We narrowed to 324 results for: cloning

...that have standard cloning sites or gateway compatible cloning sites for easy cloning of your genes of ... and repair templates of choice though modular cloning steps. The Voytas lab plans to update the toolkit...

... fluorescent proteins, synthetic biology, gene cloning and expression, as well as guide pages for Synthetic...

...naked eye. This makes cloning quite a bit easier since you can see if your cloning experiment worked just...

...H840. To ensure that the clones were capable of cleavage, they tested the clones against an NNN PAM library...

...Pathway Clone Collection 2.0. The collection is a set of vectors for use with the Gateway Cloning Platform...

... by developing generic strategies for detecting cloned genes (Munro and Pelham, 1984). The strategy they...peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila...

...their properties. Where available, we have listed cloning backbones and control plasmids. Donor Acceptor ....8 6.2 3.1 pmTurquoise2-N1 , pCS2+mNeonGreen-C Cloning Vector , mNeonGreen-mTurquoise2 CyPet YPet 435 ....80 592 128,000 0.45 6.4 4.7 pCS2+mNeonGreen-C Cloning Vector , pcDNA3-mRuby2 , mNeonGreen-mRuby2-FRET...mVenus, and Amber (mVenus Y67C) FRET standards and cloning backbones Koushik et al. (2006) Cerulean, Venus...two-hybrid FRET Positive and negative controls and cloning backbones for mTurquoise2-mVenus(L68V) Goedhart...yield of 93% Positive and negative controls and cloning backbones for various FRET pairs with mTurquoise2...mTurquoise2 Positive and negative controls and cloning backbones for red, yellow, and turquoise FRET pairs...

...the 29 SARS-CoV-2 proteins which all but one are cloned into pLVX vectors (for lentiviral packaging) and...and contain an IRES-Puro marker for stable cell clone selection. For more information on these plasmids...

...If you’ve ever used Golden Gate Assembly for cloning, you might be familiar with the rules of thumb for...complexity allowable by a favored, fairly easy cloning technique (which may be of particular interest ...

...specific genomic locus, which can require multiple cloning steps (Fujita et al., 2013). CRISPR-mediated purification...can be targeted using a gRNA, which can easily be cloned into a gRNA expression plasmid. Experimental use...

... follows a simple workflow: Clone spacer(s) into the CRISPR array Clone desired DNA cargo into the mini-transposon...

... also loved helping to answer your plasmid and cloning questions through our Plasmids 101 series and by...

...demonstrated in human cells They used the Golden Gate cloning method to ligate expression cassettes of the gRNAs...

...popular empty backbone plasmids into which you can clone your regulatory element or gene of interest into... of 5' promoter/enhancer regions using Gateway cloning. Jorge Ferrer 16539 pBV-Luc Firefly Insertion of...Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2-ccdB-Nanoluc NanoLuc...Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2-Nanoluc-ccdB...Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X Firefly...Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 106253 pLS-mP-Luc Firefly Insertion... can be used for screening of transformants in cloning process. Lynne Postovit 64034 pGL4Luc-RLuc Firefly...

...molecular biology work has focused on using various cloning methods and tools to construct chimeric cellulases...

...currently unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target... target sequence, as well as design and clone in the homology arms for the donor plasmid. The Mendenhall... also provided their protocol for homology arm cloning: Mendenhall & Myers FETCh-seq Protocol 134.7 KB... complexes. This vector serves as a backbone to clone the left and right homology arms. More details on...al. Cell Rep. 2015 . Alternatively, cDNAs can be cloned directly into this vector and targeted to the AAVS1...include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt Unc-32::GFP targeting vector...

...NNN) at any given codon site of interest can be cloned into an expression vector with Gibson assembly....Both produced about the same number of positive clones. See Supplementary Note 1 in Wrenbeck et al for...

...of E. coli for DNA propagation. While great for cloning purposes, these E. coli strains are not usually... (DE3)** Rifampicin RecA-deficient; allows for cloning and expression in same strain F- recA1 hsdR(rK12...

... To test the potential of Cas14, the Doudna lab cloned the cas14 gene together with the adjacent CRISPR...

...developed and implemented by applying the Golden Gate cloning method to human proteins known as Pumilio/fem-3...