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We narrowed to 329 results for: cloning

Showing: 141 - 160 of 329 results

  3. Genomic Deletions in Mammalian Cell Lines

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    Collection
    ...CRISPR/Cas9 Clones for Deletions and Clone Selection For suspension cells, transfer all clones to a single...deletion clones with a proofreading polymerase and clone the amplicons ( e.g., with a PCR cloning kit) into...one plate to allow for clones to grow and use the other plate to screen each clone for deletions. Add an...Validation of Biallelic Deletion Clones In order to characterize obtained clones and validate a successful knockout...present a simple methodology for CRISPR design, cloning, and delivery for the production of genomic deletions...within exons will enrich even monoallelic deletion clones for loss of function. This is due to the high frequency...complement including additional nucleotides for cloning and expression purposes. NOTE: Here we discuss ...

  4. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...related to Cloning and Genomic Tools for library construction, genomic modification, and cloning systems....Biology plasmids for cloning and genomic tools. Plasmid... Plasmid Collections Synthetic Biology Cloning and Genomic Tools...Tools Synthetic Biology: Cloning and Genomic Tools SynBio Resources SynBio Home Browse All SynBio Assembly...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...systems. Jump to Table: Cloning and Genomic Tools Reporters and Tags Library Construction Integration Recombination...Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the table by keyword or sort by...

  6. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...of E. coli for DNA propagation. While great for cloning purposes, these E. coli strains are not usually... (DE3)** Rifampicin RecA-deficient; allows for cloning and expression in same strain F- recA1 hsdR(rK12...

  7. CRISPR References and Information

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    Collection
    ...gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for Cas9 orthologs... Cas9 PDF, 66.8 KB Fujii gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo... Fly: gRNA cloning pAc-sgRNA-Cas9 PDF, 169 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF,...Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF, 129 KB Sontheimer gRNA design and cloning All-in-one ... helps design (10 different prediction scores), clone (primer design), and evaluate gRNAs, as well as ...plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; ...PDF, 110 KB Goldstein Nematode: gRNA design and cloning Cas9-sgRNA construct PDF, 364 KB Goldstein Nematode...

  11. Plant Plasmids and Resources

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    Collection
    ...Scott Lenaghan. Joint Modular Cloning (JMC) Toolkit includes a modular cloning chassis and set of functional...as the promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI Return to...Protein Guide Addgene Blog: Plant Biology CRISPR Cloning Transcription Factors RNAi Empty Backbones Fluorescent...Vladimir Nekrasov. The MoClo-compatible zCas9i Cloning Kit includes a high efficiency intron-optimized...plants (including rice and wheat) - Caixia Gao. Cloning The following collections are designed for creating... rescue in plants - Martin Parniske. GreenGate Cloning System to create plant expression vectors containing...constructs - Johannes Stuttmann. MoChlo: Modular Cloning Chloroplast Toolbox for design of synthetic operons...

  12. Zhang Lab CRISPR Page

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    Collection
    ...Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 KB SpCas9...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...annealed oligos (design is indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold...annealed oligos (design is indicated below) can be cloned into the CRISPR array. The oligos are designed ...activator helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop...Hygro resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop...for insertion of guide sequences Zhang Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome...

  13. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Additional Cloning Information Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol...provided as empty backbones that utilize Golden Gate cloning to insert the desired guide RNA sequence by digestion...Protocol (PDF, 237 KB) Dual guide cloning: Brzezinski Lab Dual Guide Protocol (PDF, 990 KB) Plasmid and Sequence...

  14. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ... mutant clones from watermelon have been codon-optimized for expression in bacteria and cloned into an...region of MDH. For detailed clone information, please see the MDH Protein Clone Information Sheet, available...malate dehydrogenase genes and watermelon mutant clones for your course-based undergraduate research experience...IPTG-inducible His-tag expression vector. All His-tags are cloned at the C-terminus of MDH and, if indicated on the...

  15. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...developed for another decade when in 1988 they were cloned by two separate labs (Bird et al., 1988; Huston...an scFv?  scFv sequences have traditionally been cloned by amplifying the VLand VH antibody sequences of...

  16. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...around 20 minutes, they are perfect for molecular cloning and metabolic engineering.  The E. coli genome ...out the GreenGate Toolkit which allows for easy cloning of constructs specifically designed for use in ...

  17. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ... al., 2017). Our vectors have two Bsa1 sites to clone the single guide RNA (protospacer+scaffold) in a...single polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables us to target...

  19. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian expression plasmid for cloning untagged proteins...Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd gen lentiviral backbone for cloning and expression ...may contain tags or fusion proteins, a multiple cloning site (MCS), an inducible promoter, and/or a selectable...with C-terminal mTagBFP2 tag pNIC-GST-TEV-GG - Cloning of target gene with N-terminal GST tag and TEV ...for bacterial expression, Ligation Independent Cloning (LIC) Also see our Bacterial Expression collection...zebrafish insulin promoter vector with multiple cloning sites Browse our full Fluorescent ...thioredoxin) Protein purification pNH-TrxT - LIC cloning in bacteria with N-terminal His-TrxA-TEV tag NusA...
Showing: 141 - 160 of 329 results