We narrowed to 324 results for: cloning

Polyclonal antibodies are a common research reagent - learn how they’re produced and the advantages and disadvantages of using them in your next experiment.

Model organisms have genetic and developmental characteristics that make them easy to study. Examples include E. coli, rat, frog, axolotl, and Arabidopsis.

Joining the popular sacB counterselection method is a new suite of toxin-based allelic exchange vector that can be used in many proteobacterial genomes.

The PUF Assembly Kit from Huimin Zhao's lab makes it possible to devise designer PUF proteins to hit any RNA target of interest.

An inside look at the ten most frequently distributed plasmid technologies at Addgene from the past ten years.

Learn about new tools in Addgene's collection, including new prime editing and CRISPR applications, tools for neuroscience research, and more!

When you have multiple antibodies to choose from, does isotype matter? Yes, and understanding how can help you pick the right antibodies for your experiment.

Epigenetic modifications are an additional layer of control over gene expression. Targeted epigenetic modification is possible using CRISPR tools from Addgene!

Discover the tools you need to perform plant genome engineering with CRISPR.

Three useful tips to help you organize your lab notebooks, keep track of tasks, and share protocols with other lab members.

Researchers inactivate genes to investigate their function. This article describes techniques/plasmids used to knockout and knock-in specific genes in mammalian cells.

Read updates on new developments in cancer research and learn about Addgene's Cancer Collection pages.

Tips to help you save time when using the the Basic Local Alignment Search Tool (BLAST) provided by NCBI to compare sequence results.

Yeast genome modification plasmids have been developed for tagging yeast genes with fluorescent proteins, epitope tags, and more. Various systems are described to help you choose which to use for your research.

Read on to learn more about this technique to isolate specific genomic regions using CRISPR.

Get the basics on RNA interference (RNAi), how it compares with CRISPR, and tools for RNAi studies in plants.

This post describes applications of pooled libraries, briefly explains several approaches, and highlights the most popular pooled libraries at Addgene.

2024 is the Year of Camelids! To celebrate, we are taking a look at how these species (e.g. camels, llamas, alpacas) have impacted biological research.

SPARC is a tool that labels a fraction of a genetically-defined subset of cells. The result: easy visualization of individual, non-overlapping cells.

Learn how to select the plasmid purification kit that best suits your needs!