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We narrowed to 230 results for: crispr c plasmids

Showing: 141 - 160 of 230 results

  1. CRISPR Guide

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    ...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software and...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...miRNAs to restrict CRISPR editing only to the target tissue. Browse Plasmids: Anti-CRISPR Small Precision...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...modification using CRISPR Browse Plasmids: Epigenetics Visualizing Genomic Loci Using Fluorophores CRISPR imaging...genomic regions using CRISPR Browse Plasmids: Purify Cas9 Alternatives for CRISPR Genome Engineering S....

  2. Antibodies 101: Validation

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    Blog Post
    ...RNAi to knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate...the gene for your protein has been mutated using CRISPR/Cas9. You check the mutation and it causes a large...interest endogenously and transfect them with a plasmid encoding your protein of interest. Then you should...target protein in a Western blot. You acquire a plasmid encoding your protein of interest, known as Gene... In parallel, you transfect cells with another plasmid not encoding this protein, which we will call Negative...band from the cells transfected with the other plasmid. Your antibody works! Multiple (Independent) Antibodies...Antibodies blog post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ...

  3. Common Injection Routes in Mice

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    Blog Post
    ...Genome Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox plasmids References Prathipati, P... P., Rodriguez-Aguayo, C., Walton, B. L., Sood, A. K., Greaver, J., Janssen, C. F., & Lopez-Berestein,... Academic Press. Turner, P. V., Brabb, T., Pekow, C., & Vasbinder, M. A. (2011). Administration of substances...

  4. Genomic Deletions in Mammalian Cell Lines

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    ...: 95 °C for 15 min, 35 cycles of (95 °C for 30 sec, 60 °C for 1 min, 72 °C for 1 min), and 72 °C for 10...following parameters: 37 °C for 30 min; 95 °C for 5 min and then ramp down to 25 °C at 5 °C/min. Dilute oligos...Mammalian Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud...usage of the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230). This plasmid allows for the simultaneous...parameters: Cycles 1-20 (37 °C for 5 min, 20 °C for 5 min); Cycle 21 (80 °C for 20 min). These cycling ...Incubate at 30 - 37 °C for 24 - 72 hr. 30 °C may enhance genome editing efficiency, but 37 °C is acceptable.... CRISPR References & Information Genomic Deletions in Mammalian Cell Lines CRISPR: Protocol...

  5. Hot Plasmids: Summer 2025

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    ...the new plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids articles...barcoding Addgene’s own AAV6 and AAV11 packaging plasmids Mapping anterograde synaptic connections with ...experiments and see the difference! Find mGold2 plasmids here! Lee, J., Lai, S., et al. (2025). Bright... TnpB, so hot right now By Alyssa Shepard While CRISPR has become a household name, the search for smaller...more in our recent blog post on TnpB). Similar to CRISPR-Cas, TnpB is directed to genomic targets using ... to the broad host range of TRV. Find TRV-TnpB plasmids here! Weiss, T., et al. (2025). Viral delivery...j.stem.2025.01.012.  New AAV6 and AAV11 packaging plasmids from Addgene By Meghan Rego Attention AAV users...

  6. CRISPR Plasmids - CRISPR Transposases (CAST)

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    ...CRISPR Plasmids: CRISPR Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base... or search the tables below for CRISPR Transposase plasmids. Plasmids are available for expression in ...Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-associated transposases (CASTs) combine the large-...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  7. Plasmids 101: Gene, enhancer, and promoter trapping

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    ...in the Lab Plasmids 101: Screens vs. Selections Plasmids 101: Knockout /Knock-In Plasmids ...the Lab Bench Plasmids 101 e-book Additional resources on the Addgene blog Plasmids 101: Using Transposons..., they eliminate the expression of the gene (by CRISPR knockout, shRNA depletion, etc.) and ask what the.... DOI: https://doi.org/10.1080/rsob.180241 Vanni, C., et al., Unifying the known and unknown microbial...

  8. CRISPR Plasmids - C. elegans

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    ... CRISPR C. elegans CRISPR Plasmids: C. ...Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in C. elegans. Cut...CRISPR plasmids that have been designed for use in C. elegans. CRISPR...Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols.... elegans Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you will...

  9. CRISPR Plasmids - Tagging

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    ...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor...Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols...the PITCh system plasmids for CRISPR-based knock-in of EGFP-2A-PuroR cassette to the C-terminus of endogenous...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR... CRISPR Protein Tagging CRISPR Plasmids...Plasmids - Protein Tagging Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR system has been adapted to allow tagging of proteins...

  10. Viral Vectors 101: The Retroviral Lifecycle

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    ...4355596 King, S. R., Duggal, N. K., Ndongmo, C. B., Pacut, C., & Telesnitsky, A. (2008). Pseudodiploid Genome...Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide References Cullen, B. R. (...Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses, 13...34372494. Prussin, A. J., Garcia, E. B., & Marr, L. C. (2015). Total Virus and Bacteria Concentrations in...Xiao, H., Conway, J. A., Hunter, E., & Kappes, J. C. (1997). Functional RT and IN incorporated into HIV...

  11. CRISPR Plasmids and Resources

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    ...bacteria. C. elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed...preps of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used ...gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed... Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for... parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in...yeast. Xenopus CRISPR plasmids designed for use in Xenopus . Zebrafish CRISPR plasmids designed for use...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...

  12. Fluorescent Proteins 101: When GFP lets you down

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    ...genomic loci in living human cells by an optimized CRISPR/Cas system." Cell 155.7 (2013): 1479-1491. PubMed...protein fusions Browse the Fluorescent Proteins Plasmid Kits ...Only about 10 amino acids can be deleted from the C-terminus and about 5 from the N-terminus, but this... standard practice to attach the GFP to the N- or C-terminus of another protein. This may, however, interfere...consensus sequence (MG-) and prenylation requires a C-terminal consensus sequence (-CaaX). Both modifications...when GFP is fused to these sequences. When N- and C-terminal fusions are not tolerated the alternative...the reasons that this works well is that the N- and C-terminus of GFP itself are relatively close, thereby...

  13. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    ...Mutate Your Plasmids Resources on Addgene.org Find Plasmids from the Whitehead Lab Use CRISPR Pooled Libraries... Can I use my favorite plasmid with this protocol? Maybe. Not all plasmids will work for the one-pot...strands of the plasmid will be nicked during the first BbvCI digest and the whole plasmid will be degraded...those in the plasmid. Again, if they are in the opposite direction, both strands of the plasmid will be nicked...map of your plasmid. If the sites are in opposite directions, you just need to use a plasmid with BbvCI...Generate Specific Mutations in Your Plasmids Learn How You Can Use CRISPR Pooled Libraries to Generate Mutant.... This technique relies on the generation of a plasmid library that expresses all desired variants of ...

  14. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    ...a given disease. CRISPR has made genomic SDM relatively straightforward, but plasmid-based SDM has lagged...recommended for plasmids up to 25 kb, most cloners should be fine, especially using plasmids in the 10-12...Other Plasmid Cloning Techniques like SLIC and Golden Gate Cloning Learn How to Verify Your Plasmid Read...Reference Pages Choose Your Perfect Plasmid Backbone Find Plasmids for Your Research ...circular plasmids - no nick repair is needed. Since the bacteria are subsequently grown at 37 °C, the recombineering... REPLACR (Recombineering of Ends of Linearized Plasmids After PCR). Current site directed mutagenesis...mutation, then use those to PCR amplify the template plasmid. The parental, nonmutated DNA is digested with ...

  15. CRISPR Plasmids - Cascade-Cas3

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    ... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3... or search the tables below for CRISPR Cascade-Cas3 plasmids. Plasmids are available for expression in...Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR...Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Protocols gRNA Design Tools CRISPR Blog Posts Cas3 is a unique member of the CRISPR family. After nicking the...to express equally. To combat this, some CRISPR-Cas3 plasmids take an all-in-one approach and include ...

  16. CRISPR Plasmids - Double-Strand Break (Cut)

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    ... CRISPR Cut CRISPR Plasmids: Cut Browse CRISPR...search the tables below for CRISPR plasmids designed to introduce a DSB. Plasmids are available for expression...Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols...Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans ID Plasmid Gene/Insert...CRISPR plasmids that create double strand breaks in DNA. CRISPR...Protocols gRNA Design Tools CRISPR Blog Posts Fully functional CRISPR/Cas enzymes will introduce a double-strand...Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter...

  17. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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    ...organism.   It’s not an overstatement to say that CRISPR/Cas9 editing is one of the key tools now providing...plans and in particular, that they all share the plasmids created under this programs via Addgene. New organisms...clutch of eggs? Propagation is thus quite difficult. CRISPR genome editing in squid is possible nonetheless...reptiles at all. But new success is coming with CRISPR/Cas in lizards. The Menke Lab was able to make ...sexual reproductive life cycles. One can inject plasmid DNA in the eggs to get transgenic animals. They...habitats - even 50 meters from Old Faithful in 60 °C soils. Dendrobatidae (Lauren O’Connell)  Poison frogs...

  18. Important Considerations When Using AAVs

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    ...system. Currently, AAVs are being used to deliver CRISPR/Cas9 but have the potential to deliver other therapeutic...be used with new exciting technologies (such as CRISPR/Cas9) as they allow for gene delivery in an efficient...Gene Expression in Mammals A Match Made in Heaven: CRISPR/Cas9 and AAV Viral Vector Elements Additional ...Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...nitrogen or a dry ice/ethanol bath, and store at -80° C. Once thawed, the AAV aliquot should be used quickly...PubMed Central PMCID: PMC190186. 9. Grieger, Joshua C., and Richard J. Samulski. "Packaging capacity of ...

  20. CRISPR Plasmids - Base Edit

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    ...CRISPR Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit... or search the tables below for CRISPR base editing plasmids. Plasmids are available for expression in...Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break. CRISPR...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...
Showing: 141 - 160 of 230 results