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Showing: 141 - 160 of 960 results

  1. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ... [+] and [-] strands might naturally associate forming a double-stranded species that could be used as...establish a next-generation sequencing (NGS) QC platform that allows us to provide scientists with complete...launching our viral service in 2016, we adapted this platform to accommodate adeno-associated viral vector (...

  2. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...encouraging user contributions (using simple web forms) while also maintaining a relatively strict database...downloaded in JSON or CSV format to quickly retrieve all of the information in the database relating to... point.  However, there is a great deal more information that one might like to store about FPs than is...currently represented at fpvis (complete spectral information, accession numbers, sequences and lineages, FRET...community-editable resource for storage and dissemination of information about FPs. FPbase.org FPbase is a project written...eventually be a comprehensive community resource for information about fluorescent proteins.  It was designed ... most obvious use case for FPbase is finding information on a particular FP; this can be done directly...

  3. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...basepairs adenine (A) and thymine (T) to hybridize and form hydrogen bonds. This post focuses on "sticky end... allowing a researcher to perform blue-white screening after transformation - self joining of the vector...planning to transform right away OR you can store the reaction at -20C overnight. 4. Transform TOPO Cloning... minutes will not significantly improve the transformation efficiency).  5. Select and Analyze 10 White...add will depend on whether you are planning on transforming your reaction into chemically or electro-competent...that you exceed the 5 minute time limit (lower transformation efficiencies have been reported with longer...antibiotic-containing plate prior to plating your transformation may allow you to see colonies within 8 hours...

  4. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    ...persistent identifiers? Perhaps the most commonly known form of a unique PID is the Digital Object Identifier... well known PID formats are resolvable compact identifiers, which are Compact Uniform Resource Identifiers...both of these PID formats for lenti-Cas9-VQR contain the Addgene ID # and the information necessary to indicate...through a paper to find that there is insufficient information about which reagents were used? Unambiguous identification... available through Addgene and find verified information about each plasmid item. Given a specific Addgene...page on the Addgene website and find specific information about the plasmid: name, description, how the...the plasmid was generated, validated sequence information, and more. The plasmid page also helps researchers...

  5. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...interests come together as useful and productive, forming a different constellation of interests and skills...caught me early on: a simple egg is packed with information. I am fascinated with how developmental programs... how open access affects financial models in information publication. Take a look at the blog ‘Scholarly...

  8. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ...and life goals. For tips on how to get started see Form Your Own Peer Mentoring Group: A How-To Guide for...your hand at a video presentation? Most video platforms make it easy to record yourself. You can watch...demonstrated initiative. In fact, hear from Addgene’s former blog editor Tyler Ford on how initiative landed...you get the idea. What will you start? 4. Do informational interviews (on a schedule) I hope you all know...until you imminently need a job. Start now. Informational interviews are 1:1 discussions with someone ...builds a relationship for the future and the 1:1 format is good for everyone including those that find ... speak with. See this post that’s all about informational interviews, for more details on how to make ...

  9. Plasmid Cloning by PCR

    Type
    Blog Post
    ...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time... the end of a linear piece (see NEB for more information). Thus, we recommend that you add 3-6 bases upstream...should ensure that the bases do not result in the formation of a hairpin structure within your primer. In ...

  10. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...rates and discovered that the catalytically active form of the nuclease dissociates very slowly after introducing... editing experiments in this publication were performed using a technique called nucleofection, which ...found on our plasmid pages. We encourage any labs performing genome editing experiments to try nucleofection...

  11. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...a spreadsheet. The plasmid information can be entered using our online form or by using a downloadable...button to download the .xls file. For the online form you can submit "Published plasmids" or "Unpublished...would prefer to send us your plasmid information in spreadsheet format, e-mail us at  [email protected]" button to add your plasmid information. Enter plasmid information During the remainder of the process... Figure 7: Enter information about the gene/insert(s). Step 3: Cloning Information Please enter the name...of the information on this page to make sure it is correct. You can easily update the information by navigating...submission, we are happy to update the publication information once the manuscript is published in a peer-reviewed...

  12. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...Immune Reactions Anti-drug antibodies (ADAs) are formed when the body recognizes a drug as a foreign antigen...therapeutic antibody itself or as impurities in the formulation. Efforts to predict T cell epitopes focus on ...evaluation of ‌therapeutic immunogenicity is typically performed using traditional 2D assays with immune cell lines...their own limitations. Using methods together can inform decisions about in vivo application and clinical... medicine in the future.   Madhura Acharya is a former co-op and current Scientist, DNA Sequencing and... & Schellens, J. H. (2016). Antidrug Antibody Formation in Oncology: Clinical Relevance and Challenges...

  14. Hot Plasmids - January 2023

    Type
    Blog Post
    ...against full length GFP and converted to recombinant form by the NeuroMab Facility Isotype IgG2a Differences...Another reason is that some antibodies may just not perform as well in one application as another, so it is...

  17. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    ...experiments and published your data, please fill out this form and we’ll add your citation to the plasmid page ... pass QC. The lab team manually transforms, innoculates, and performs DNA minipreps for small batches ...standard pipeline. For the first ~50 plasmids we perform our standard full plasmid sequencing by NGS. Addgene...and make them available to scientists without performing the full suite of time consuming, costly, and...intensive screening and the use of high throughput platforms, Addgene and Ginkgo are striking a balance by ...

  20. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...distribute many of these technologies in plasmid form and are proud to have facilitated their fast adoption...lentiviral vectors from the repository for you. We perform test infections with all of our viral preps and...variety of Cas9 activators available in lentiviral format. Finally, while we can’t currently produce custom...Virus Pooled libraries now available in lentiviral format Another popular set of tools in the repository ...after receiving a pooled gRNA library in plasmid format, a researcher must amplify the library, use next-generation.... We provide enough lentivirus for you to both perform an optimization-scale infection and at least one...the pooled libraries we distribute in lentivirus format only deliver gRNA and therefore, must be used to...
Showing: 141 - 160 of 960 results