We narrowed to 243 results for: grn

1. Adeno-associated Viruses (AAVs) for Genome Editing

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   ...use a dual vector system composed of SaCas9 and a gRNA in one AAV and a repair template in second a second...small Cas9 homologs like SaCas9 and their requisite gRNAs take up essentially all of an AAV’s ~4.5 kB packaging...

2. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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   ...about High Fidelity Cas9 Variants How to Design Your gRNA Delivering CRISPR Ribonucleoproteins Resources ...Check Out Our CRISPR Guide Pages Find Validated gRNAs ...

3. CRISPR 101: Cytosine and Adenine Base Editors

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   ...nickase) fused to a deaminase that makes the edit A gRNA targeting Cas to a specific locus A target base ...CRISPR plasmids for base editing Find validated gRNAs ...

4. The 12 Days of CRISPR: 2021

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   ... day of CRISPR, Addgene gave to me: engineered pegRNAs to improve prime editing efficiency. On the third...

5. Twelve Amazing Guest Blog Contributors!

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   ...software that predicts efficiencies of prime editing gRNAs.   PRIDICT Harvinder Virk and Michael Biddle, ...

6. Performing In Vivo CRISPR Screens Using the FITS Approach

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   ...types. A key feature of this system is a lentiviral gRNA expression vector (pRDA_526) that (1) enables efficient...

7. Addgene’s Top 10 Blog Posts from 2014

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   Blog Post

   ...Most Popular Posts of 2014 How to Design Your gRNA for CRISPR Genome Editing By John Doench (Guest...

8. Editor's Choice, August 2016

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   Blog Post

   ...the transitions at target sequences specified by gRNAs. Caveats, potential specificity issues, and other...

9. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

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   ..., programmable DNA cutting enzyme. Second, since gRNA and crRNA could be combined into one RNA to direct...

10. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    ...smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of interest. Promoter The promoter...

11. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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    ... protein is 1,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is thermostable ...

12. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

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    Blog Post

    ... Z, Ma X, Wang N, Huo Y-X (2020) Reversed paired-gRNA plasmid cloning strategy for efficient genome editing...

13. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    ...the case of retroviruses, reverse transcribe their gRNA into DNA, which is then transcribed into mRNA before...

14. Using Addgene's New Viral Service for Your Research

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    ...nickase, Cas9 activators, Cas9 repressors, select gRNAs, and CRISPR pooled libraries. Whether you’d like...

15. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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    ...3xFLAG-tag fusion proteins. The repair template and Cas9-sgRNA plasmid is then injected into the germline of young...

16. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    ...call the LADL Anchor. Using specifically designed gRNAs, they can then recruit these LADL Anchors to genomic...

17. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    ...impact of off-target mutations, scientists are using sgRNA for selection and optimization. In addition, the...

18. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    ...with CasRx and either an unprocessed or processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating...

19. Improved Plasmid Maps Powered by SnapGene

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    Blog Post

    ...commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display, you...

20. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post

    ...γ-Retroviral transfer plasmid encoding a transgene, sgRNA, or shRNA of interest: The transgene sequence is...