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  1. Communicating Your Science With Help From ComSciCon

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    Blog Post
    ...throughout the year.  Find workshops in your area here: https://comscicon.com/. All our events are offered free...fantastic K12 education initiatives like BiteScis (https://bitescis.org/) and amazing graduate student writing...writing collaboratives like Oceanbites (https://oceanbites.org/) have already grown out of our workshops...

  2. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    Blog Post
    ...Frontiers in Cell and Developmental Biology 6: . https://doi.org/10.3389/fcell.2018.00054 Sander JD, ...targeting genomes. Nature Biotechnology 32:347–355 . https://doi.org/10.1038/nbt.2842 Wen Y, Liao G, Pritchard...Journal of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074/jbc.m117.777722 Additional resources...

  3. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...Acids into Proteins. Science, 244(4901), 182–188. https://doi.org/10.1126/science.2649980 Shandell, M. A...and Perspective. Biochemistry, 60(46), 3455–3469. https://doi.org/10.1021/acs.biochem.1c00286 Zhu, P., Mehl...Recombinant Proteins in Escherichia coli. 12(21). https://doi.org/10.21769/BioProtoc.4541  ...

  4. Antibodies 101: Fab Fragments

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    Blog Post
    ...Prospects of Antibody Fragments. Antibodies 8(2):28. https://doi.org/10.3390/antib8020028    Wessel GM, McClay...Histochem Cytochem : Off J Histochem Soc 34(6):703–706. https://doi.org/10.1177/34.6.3084626  Zhang Q, Miyamoto...super-resolution microscopy. Cell Rep Methods 2(10):100301. https://doi.org/10.1016/j.crmeth.2022.100301  Additional...

  5. Hot Plasmids - October 2020

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    ...possible.   Bensussen S, et al., iScience 2020. https://doi.org/10.1016/j.isci.2020.101330 Split-wrmScarlet...Goudeau et al., 2020. Goudeau, J. bioRxiv 2020. https://doi.org/10.1101/2020.07.02.185249 Predicting ...proliferate. Eastman et al. Cell Reports 2020. https://doi.org/10.1016/j.celrep.2020.107804 The CRISPR...

  6. High Complexity Golden Gate Assembly with NEB’s DAD

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    Blog Post
    ...Application to DNA Assembly. ACS Synthetic Biology. https://doi.org/10.1021/acssynbio.8b00333 Pryor, J. M....Design. ACS Synthetic Biology, 11(6), 2036–2042. https://doi.org/10.1021/acssynbio.1c00525 Pryor, J. M....data-optimized assembly design. PLOS ONE, 15(9), e0238592. https://doi.org/10.1371/journal.pone.0238592    ...

  7. Antibodies 101: Choosing the Right Isotype

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    Blog Post
    .... 46, Issue 8, pp. 977–983). SAGE Publications. https://doi.org/10.1177/002215549804600813 Erickson, P...Methods in Cell Biology (pp. 283–310). Elsevier. https://doi.org/10.1016/s0091-679x(08)60255-1  Manning..., p. e38313). Public Library of Science (PLoS). https://doi.org/10.1371/journal.pone.0038313 More resources...

  9. AAV Vector Quality Control: Going the Extra Mile with NGS

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    Blog Post
    ...Vector Delivery. Molecular Therapy 12:744–753 . https://doi.org/10.1016/j.ymthe.2005.06.003 Guerin K, ...Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum.2019.277 Lecomte E, Tournaire...Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Wright JF, Zelenaia...

  10. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    Blog Post
    ...organization deciphering. Molecular Cytogenetics, 11. https://doi.org/10.1186/s13039-018-0368-2  Liu X, Zhang...by Biotinylated dCas9. Cell 170:1028–1043.e19 . https://doi.org/10.1016/j.cell.2017.08.003 Liu, X., Chen...chromatin by biotinylated dCas9. Genome Biology, 21. https://doi.org/10.1186/s13059-020-01973-w Additional ...

  11. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post
    ...Journal of Clinical Microbiology, 23(5), 959–961. https://doi.org/10.1128/jcm.23.5.959-961.1986 Lobstein...its cytoplasm. Microbial Cell Factories, 11, 56. https://doi.org/10.1186/1475-2859-11-56 Trastoy, M. O....cleavage in human cells. Mutagenesis, 20(2), 111–114. https://doi.org/10.1093/mutage/gei016I Resources on Addgene.org...

  12. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...viral vector production. Neuromethods, 195, 77–101. https://doi.org/10.1007/978-1-0716-2918-5_5 Haery, L.,...Neuronal Manipulation. Frontiers in Neuroanatomy, 13. https://www.frontiersin.org/articles/10.3389/fnana.2019.00093...Protocols in Molecular Biology, 115, 31.5.1-31.5.13. https://doi.org/10.1002/cpmb.8...

  13. CRISPR-based Genome Editing Systems For Editing in E. coli

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    ...CRISPR-Cas9 System. Appl Environ Microbiol 81:2506–2514 . https://doi.org/10.1128/aem.04023-14 Li Q, Sun B, Chen...Escherichia coli. Acta Biochimica et Biophysica Sinica. https://doi.org/10.1093/abbs/gmab036 Zhang Y, Sun X, Wang...CRISPR-Associated Transposases. ACS Synth Biol 9:1998–2008. https://doi.org/10.1021/acssynbio.0c00073   Additional...

  14. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post
    ...Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/978-1-4939-8935-5_26 Stuurman ...Introduction to Fluorescence Microscopy. iBiology. https://www.ibiology.org/talks/fluorescence-microscopy...validation of antibodies. Nat Methods 13:823–827. https://doi.org/10.1038/nmeth.3995 Additional Resources...

  15. Antibodies 101: Normalization and Loading Controls for Western Blots

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    ...Journal of Neuroscience Methods, 172(2), 250–254. https://doi.org/10.1016/j.jneumeth.2008.05.003 Ghosh, ...blots. Expert Review of Proteomics, 11(5), 549–560. https://doi.org/10.1586/14789450.2014.939635 Gilda, J....blots. Analytical Biochemistry, 440(2), 186–188. https://doi.org/10.1016/j.ab.2013.05.027 Additional Resources...

  16. Plasmids 101: Repressible Promoters

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    Blog Post
    ... Tet-On and Tet-Off systems: Second generation. https://www.takarabio.com/products/gene-function/tet-inducible-expression-systems...in mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750-6-43Kaczmarczyk A, Vorholt...overexpression transgenes. Sci Rep 12, 3835 (2022). https://doi.org/10.1038/s41598-022-07852-7 De Boer, Herman...

  17. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    ...Mammalian Cell Culture. Anal Chem 84:4227–4232 . https://doi.org/10.1021/ac2033112 Ligasová A, Vydržalová...Mycoplasmas Using Fluorescence Microscopy. Cells 8:1510 . https://doi.org/10.3390/cells8121510 Sung H, Kang SH, ...Cell Lines. In Vitro Cell Dev Biol Anim 38:86 . https://doi.org/10.1290/1071-2690(2002)038<0086:caeomi...

  18. Antibodies 101: Flow Cytometry

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    .... Review of Scientific Instruments 43:404–409 . https://doi.org/10.1063/1.1685647 McKinnon KM (2018) Flow...Overview. Current Protocols in Immunology 120: . https://doi.org/10.1002/cpim.40 Picot J, Guerin CL, Le...recent instrumentation. Cytotechnology 64:109–130 . https://doi.org/10.1007/s10616-011-9415-0 Additional resources...

  20. Congratulations, Deck The Lab winners!

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    Blog Post
    ... #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com/OOrwo9feyJ — BTC...Remind them you care!#thanks #health #mentalhealth https://t.co/Td2M6AOpuW pic.twitter.com/ofLI4eD8Zs — Microbigals...
Showing: 141 - 160 of 255 results