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We narrowed to 522 results for: pes;

Showing: 141 - 160 of 522 results

  1. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    ...antibodies bind different protein sequences called epitopes and scientists can use these to tag their proteins...of an antibody that recognize and bind specific epitopes (Ewert et al., 2004). This scFv hybrid provides... HA frankenbody is that it remains bound to HA epitopes for minutes in living cells, meaning that scientists... stitching technique could be applied to other epitopes and scaffolds to build a more robust set of frankenbodies...

  2. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...adeno-associated viral vectors (AAV) not all serotypes are equal; while some serotypes are typically high yielding, for...improve AAV yields. Although we only investigated 3 serotypes it’s possible that this approach will be beneficial...beneficial for a host of other AAV serotypes; it will be exciting to test this approach with different capsids...

  3. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...both types of HMO biosensors and displayed her findings at the Spring 2017 ACS meeting. She hopes these...advantage over other inducible enzyme technologies. She hopes that other researchers will be able to use similar.... In addition to her research endeavors, Fatima hopes to practice her science communication skills throughout...

  4. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...repressor protein absent, transcription is turned ON. Types of inducible promoters Chemical agents, temperature... you’ll find a short description of these three types of inducible promoters, and examples of each type...Because transcription machinery differs between cell types or organisms, promoters are similarly variable. ...derived. Similarly, the various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique ...

  5. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...multiple AAV serotypes (Wilmott et al., 2019), and different combinations of ITR and capsid serotypes vary in...Created with biorender.com. ITR modifications and types  Since ITRs are one of the most important elements...altered by using ITR sequences from different AAV serotypes that vary in their RBE and T-shaped hairpin loop...

  6. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...While promoters and AAV serotypes can sometimes be used to target tissue types, recombinases allow you...expression in target cells. While the various AAV serotypes can be used to achieve different patterns of expression... to offer many of our control plasmids in new serotypes, as well as a new Cre dependent GFP control plasmid...

  7. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ...molecular signatures of variant function and cancer phenotypes. Broad Institute Target Accelerator Plasmid ...Collection) or “pan-cancer” analyses across many cancer types (Pan Cancer Mutant Collection). In addition, we ...measuring a variety of molecular signatures or cancer phenotypes. These assays were designed to complement the...mutations and map other cellular functions and phenotypes. Since time is of the essence in clinical settings...

  8. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... particular signaling pathways in specific cell types (typically neurons). While all of these viruses ...and in vivo (Krashes et al., 2011). The various serotypes available (AAV2, AAV5, AAV8) enable different ... and transgene expression across different cell types (Aschauer et al., 2013). Expression of hM4D(Gi)-...efficiency, tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse brain. PLoS One...

  9. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...can be widely utilized across many different cell types and each one requires different elements for vector...independently replicated as an episome. The four main types of yeast plasmids are defined below: Yeast Integrating.... S. cerevisiae no  no TRP1 alters some yeast phenotypes. This marker should not be used in gene disruption...potential metabolic burden on the yeast cells. Some phenotypes may be altered due to the presence of the selection...

  10. All in a Twist: dsRNA

    Type
    Blog Post
    ...viral infection Viral genomes come in all sorts of shapes and sizes. Many are encoded by single stranded ... made it into agriculture as species-specific pesticides and insecticides, an advancement over previous... same inflammatory response can activate. These types of cellular dysregulation have been documented in...forms of lupus and rheumatoid arthritis. These types of immune responses are also a concern when it comes...

  11. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...to express a variety of proteins in single cell types in the mouse, but knew it would take years to develop...established mouse lines expressing GFP in specific cell types to do more than just label cells. If GFP could be...see if they could apply GFP regulation to other types of proteins. They started with another biological...their scaffold system is likely applicable to many types of proteins. For proteins with a modular structure...

  12. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ... also capable of infecting a wide range of cell types including but not limited to muscle, liver, and ...brain cells. There are a multitude of known AAV serotypes that have slight variations in their viral capsids...over many years in postmitotic, long-lived cell types. AAVs in the clinic Thus far, AAV vectors AAV1,...prevalence of neutralizing antibodies against some AAV types, AAV1, 2, 3 and 5 are thought to be as high as 70%...

  14. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...same holds true for types of viruses generated in the lab. The four most common types of viruses grown in...involve needles or scalpels (sharps). Cuts and scrapes from sharp equipment are one of the most common...Resources on the Addgene blog Viral Vectors 101: Types of Viruses Viral Vector Elements Tips for Preventing...

  15. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...from cells or using live cells. There are several types of expression systems used for protein production...post-translational modifications. There are two types of insect expression systems; baculovirus infected...integrated into the insect cell genome. Both of these types of insect expression systems can be scaled up for...of recombinant proteins. Many cells from various types of plants such as maize, tobacco, rice, sugarcane...

  16. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...Why Expansion Microscopy?  Traditional light microscopes are limited in the level of resolution that they...you might expect them to dramatically limit the types of labels you could use. But many ExM protocols ...reagents, straightforward protocols, and standard microscopes sounds great, right? But there is always a catch...loss of protein during digestion, or masking of epitopes during sample anchoring and gel polymerization...

  17. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...proteins and small molecules from a variety of sample types (Engvall 1971). Scientists value ELISAs for their...Herein, we’ll give you a rundown of the different types of ELISA, advantages and disadvantages of each, ...detection methods.      Figure 1: The four types of ELISA are direct, indirect, sandwich and competitive...antibody must bind to distinct, non-overlapping epitopes so that they do not interfere with each other....

  18. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...instead of fluorescent markers. There are various types of SSC and FSC that can be used for this purpose...). This gating strategy is used to identify two types of dendritic cells (DCs), mDCs and pDCs. Let's follow...Cells are gated, and in the gated population, two types of FSC are used to separate single cells from doublets...under Creative Commons license.    Pro tip! Subtypes of immune cells are often defined by a subset ...

  19. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ... that the late replication that occurs for some types low copy plasmids during cell division simply does...microbiological world. (Want to know more about these plasmid types? Read our Plasmids 101: Environmental Plasmids blog... plasmids from the bacterial pathogens Yersinia pestis, Agrobacterium tumefaciens, and Bacillus anthracis...) Curing of four different plasmids in Yersinia pestis using plasmid incompatibility. Letters in Applied...

  20. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...Differences in the physiology of different cell types, however, can make this assumption incorrect. One...lentiviral vectors are engineered with non-native envelopes, a process called pseudotyping. Lentiviruses pseudotyped... cell type may not translate well to other cell types. To overcome this issue, try transducing with a ...PMC1368960. 10. Denning, W., Das, S., Guo, S., Xu, J., Kappes, J.C., Hel, Z. “Optimization of the transductional...
Showing: 141 - 160 of 522 results