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We narrowed to 208 results for: pme i

Showing: 141 - 160 of 208 results

  1. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...101: An Introduction to AAV References Asencor, A. I., Dvoryanchikov, G., Makhoul, V., Tsoulfas, P., & ...., Keast, J. R., Miller, C. T., Fox, A. S., Chiu, I. M., & Gradinaru, V. (2022). Engineered AAVs for non-invasive....ymthe.2019.02.013 Huang, Q., Chan, K. Y., Tobey, I. G., Chan, Y. A., Poterba, T., Boutros, C. L., Balazs...Azari, B., Zheng, Q., Beddow, T., Zhao, B., Tobey, I. G., Moncada-Reid, C., Eid, F.-E., Walkey, C. J., ..., J., Zheng, Q., Kozareva, V., Beddow, T., Tobey, I. G., Pacouret, S., Chen, A. T., Chan, Y. A., Ryvkin...Brown, A. R., Shin, N., Kim, Y., Toong, N., Kaplow, I. M., Wirthlin, M., Zhang, X., Phan, B. N., Fox, G.... L., Krunnfusz, A., King, E. M., Ye, S., Solomon, I. H., Tabebordbar, M., & Sabeti, P. C. (2023). Systemic...

  2. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ... be flat out better than SpCas9. I want everyone to test it because I want to know the answer", says Liu...changes compared to SpCas9 in parentheses. The development of xCas9 3.6 and 3.7 shows that we have only ...

  3. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...-stimulation (ICSS) and place preference. The lab I work in (the Kreek lab) focuses on the neurobiology...opsins." Nature methods 9.2 (2012): 159-172.5. Goshen I, et al. Dynamics of retrieval strategies for remote...high-light sensitivity optical neural silencer: development and application to optogenetic control of non-human...enlightenment age for zebrafish neurobiology." Developmental neurobiology 72.3 (2012): 404-414. PubMed PMID...

  4. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...technologies are still being developed today. In this post, I will highlight the evolution of the  Brainbow multicolor...Rotkopf S, Alexandre C, Bell DM, Dickson BJ, Salecker I. Nature Methods. 2011 Mar;8(3):260-6. doi: 10.1038... were very time-intensive, necessitating the development of a new technique. In a structure as tightly...lineage tracing; “Zebrabow” was used to trace the development of the corneal epithelium. For scientists interested...interested in neuroscience and development, Brainbow is a valuable tool to mark and follow single cells... Ciruna B, Sanes JR, Luchtman JW, Schier AF. Development. 2013 Jul;140(13):2835-46. doi: 10.1242/dev.094631...

  5. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...should I use for my experiment? When I am contemplating which microscope to use for a new sample I always...can be used to overcome this (8). The recent development of tissue clearing techniques (9) now allows ...

  6. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...journal.pgen.1010765.  Berlew, E. E., Yamada, K., Kuznetsov, I. A., Rand, E. A., Ochs, C. C., Jaber, Z., Gardner,....7554/eLife.84364.  Meiring, J. C. M., Grigoriev, I., Nijenhuis, W., Kapitein, L. C., & Akhmanova, A. ...critical when, say, the gene is essential during development and a knockout is not feasible or when the protein...Rab proteins during a key stage of zebrafish development (Aljiboury et al., 2023). Rab family proteins...formation during zebrafish left-right organizer development. PLoS Genetics, 19(5), e1010765. https://doi....

  7. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ... the concentration of the unknown sample.  How do I make the standards for a standard curve? You create...should be within 15% of the expected value. How do I plot and use a standard curve? To create a standard...depending on a number of factors, including the user, equipment, assay incubation time, etc., which can vary even...

  8. The Challenges of Cell Culture

    Type
    Blog Post
    ...the American Type Culture Collection aka ATCC where I work. It is virtually impossible to work in cell culture...virology without acquiring materials from this bank. I am often asked, “What is the difference between materials...backup liquid nitrogen store to prevent loss from equipment malfunctions. One Master vial can then be thawed...

  9. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...Gaudelli et al., 2017 Adenine base editors (ABE7.10)   A→I (A→G) editing with high product purity and low off-target..., H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017). Programmable base editing of...Murray, R., Packer, M. S., Rinaldi, C., Slaymaker, I. M., Yen, J., Young, L. E., & Ciaramella, G. (2020...elements were the starting point towards the development of the first cytosine base editors from Alexis... base editing scope and efficiency Since the development of BE3, many research groups have made improvements...guanine (Thuronyi et al., 2019). Following the development of adenine base editors (discussed next!), RNA...constrained by PAM availability in the genome. So the development of near-PAMless Cas9 variants significantly expands...

  10. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ... Store Your Plasmid DNA? by Paolo Colombi Should I store my plasmid in TE, Tris, or water? And what about...blog post with creative ways to continue career development virtually. 6. Quantifying DNA? Here are Five...

  11. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ..., Isern S, Michael SF, Lorenzana I, Yozwiak NL, MacInnis BL, Bosch I, Gehrke L, Zhang F, Sabeti PC (2018...-2 RNA, but inadequate access to reagents and equipment has become a bottleneck. In the last few years...

  12. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...factors contribute to the popularity of GFP including (i) fast and complete maturation to functional, fluorescent...discussed below. There are numerous GFP variants. Here I use the name ‘GFP’, which refers to mEGFP. This variant...entirely genetically encoded do not exist, but the development of non-natural fluorescent amino acids and their...

  14. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...doi.org/10.1038/s41467-019-13805-y Doench JG (2017) Am I ready for CRISPR? A user’s guide to genetic screens...s41587-020-0490-7 Radzisheuskaya A, Shlyueva D, Müller I, Helin K (2016) Optimizing sgRNA position markedly...is still the most widely-used Cas enzyme, the development of SaCas9, NmeCas9, Cas12a enzymes, and engineered...

  15. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...quickly identify possible gene function. In this post, I will describe a useful and cost-effective method that...Thermo works well) with some added lysozyme, DNAse I, and protease inhibitor cocktail. Line the bottom ...Jessica C. Sacher, and Christine M. Szymanski. "Development of an Assay for the Identification of Receptor...

  16. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...Interest Waiver (NIW) allows for self-sponsorship of the I-140 application meaning that the category does not...and should help facilitate the interview process. I often suggest to international candidates that they...writing, clinical, regulatory, product development, business development, consulting, policy, big data and ...presented may not reflect the most current legal developments.  Further, this post may contain technical inaccuracies...necessary and often overlooked part of career development for foreign national researchers and entrepreneurs... Careers is a life science search and career development firm focused on connecting innovative, entrepreneurial...project management, alliance management, business development, finance, and operations. To learn more, visit...

  17. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...pnas.1915974116 Guerin K, Rego M, Bourges D, Ersing I, Haery L, Harten DeMaio K, Sanders E, Tasissa M, Kostman... Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020) A Novel Next-Generation...classes of neurons transiently express TH during development or have low levels of TH mRNA without expressing...

  18. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ... Career Coaching for Scientists: Why and Where Do I Find One? If you need help making a transition into...the coming years as we stay on top of the many developments in science and the plasmids, technologies, and...

  19. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ... to major histocompatibility complexes (MHC class I and II).  There are multiple algorithms, both open-access....), The Nonhuman Primate in Nonclinical Drug Development and Safety Assessment (pp. 519–542). Academic....2018.06.008Lu, RM., Hwang, YC., Liu, IJ. et al. Development of therapeutic antibodies for the treatment of...

  20. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...researchers to use this tool in their own work. She says, “I spend a lot of time packing, shipping, and sharing...at a variety of questions surrounding cancer development, metastasis, and drug resistance, Bhang says ...appropriate, non-overlapping targets for drug development - such that initial resistant populations can...tumor heterogeneity to drug discovery and the development of combination therapies.         References ...
Showing: 141 - 160 of 208 results